Immuno-flow cytometric detection of the ichthyotoxic dinoflagellates Gyrodinium aureolum and Gymnodinium nagasakiense: Independence of physiological state

Journal of Sea Research

Volumn 37, Issue 1-2, 1997, Pages 91-100

  Vrieling, Engel G ^a   Van de Poll, Willem H ^a   Vriezekolk, Gertie ^b   Gieskes, Winfried W C ^a  

^a UNIVERSITY OF GRONINGEN   (Netherlands)

^b Ministry of Transport Public Works and Water Management Netherlands   (Netherlands)

Author keywords

Antigen expression; Flow cytometry; Gymnodinium nagasakiense; Gyrodinium aureolum; Ichthyotoxic dinoflagellates; Immunofluorescence; Monitoring

Indexed keywords

GYMNODINIUM NAGASAKIENSE; GYRODINIUM AUREOLUM;

EID: 0030925493     PISSN: 13851101     EISSN: None     Source Type: Journal    
DOI: 10.1016/S1385-1101(96)00006-8     Document Type: Article

References (26)

1. Anderson, D.M., 1995. Identification of harmful algal species using molecular probes: An emerging technology. In: P. Lassus, G. Arzul, E. Erard, P. Gentien and C. Marcaillou (Editors), Harmful Marine Algal Blooms. Lavoisier, Paris, pp. 3-13.
2. Anderson, D.M., Keafer, B.A., Kulis, D.M., Waters, R.M. and Nuzzi, R., 1993. An immunofluorescent survey of the brown tide chrysophyte Aureococcus anophagefferens along the northeast coast of the United States. J. Plankton Res., 15: 563-580.
3. Bates, S.S., Léger, C., Keafer, B.A. and Anderson, D.M., 1993. Discrimination between domoic-acid-producing and non-toxic forms of the diatom Pseudonitzschia pungens using immunofluorescence. Mar. Ecol. Prog, Ser., 100: 185-195.
4. Brockmann, U.H., Laane, R.W.P.M. and Postma, H., 1990. Cycling of nutrient elements in the North Sea. Neth. J. Sea Res., 26: 239-264.
5. Dahl, E. and Tangen, K., 1993. 25 years experience with Gyrodinium aureolum in Norwegian waters. In: T.J. Smayda and Y. Shimizu (Editors), Toxic Phytoplankton Blooms in the Sea. Elsevier, Amsterdam, pp. 15-21.
6. Guillard, R.R.L., 1975. Culture of Phytoplankton for Feeding Marine Invertebrate Animals. Plenum, New York, NY.
7. Hiroishi, S., Uchida, A., Nagasaki, K. and Ishida, Y, 1988. A new method for identification of inter-and intra-species of the red tide algae Chattonella antiqua and Chattonella marina (Raphidophyceae) by means of monoclonal antibodies. J. Phycol., 24: 442-444.
8. Hofstraat, J.W., Van Zeijl, W.J.M., De Vreeze, M.E.J., Peeters, J.C.H., Peperzak, L., Colijn, F. and Rademaker, T.W.M., 1994. Phytoplankton monitoring by flow cytometry. J. Plankton Res., 16: 1197-1224.
9. Nielsen, M.V., 1992. Irradiance and daylength effects on G. aureolum. J. Plankton Res., 14: 811-820.
10. Nielsen, M.V. and Tonseth, C.P., 1991. Temperature and salinity effect on growth and chemical composition of Gyrodinium aureolum Hulburt in culture. J. Plankton Res., 13: 389-398.
11. Orellana, M.V. and Perry, M.J., 1995. Optimization of an immunofluorescent assay of the internal enzyme Ribulose-1,5-biphosphate carboxylase (Rubisco) in single phytoplankton cells. J. Phycol., 31: 785-794.
12. Partensky, F. and Vaulot, D., 1989. Cell size differentiation in the bloom-forming dinoflagellate Gymndinium cf. nagasakiense. J. Phycol., 25: 741-750.
13. Partensky, F., Vaulot, D. and Videau, C., 1991. Growth and cell cycle of two closely related red tide-forming dinoflagellates: Gymnodinium nagasakiense and G. cf. nagasakiense. J. Phycol., 27: 733-742.
14. Peeters, J.C.H., Haas, H.A., Peperzak, L. and De Vries, I., 1993. Nutrients and light as factors controlling phytoplankton biomass on the Dutch Continental Shelf (North Sea) in 1988-1990. Rep. DGW-93.004: Tidal Waters Div., Ministry of Transport, Public Works and Water Management, The Hague, pp. 1-64.
15. Sako, Y., Adachi, M. and Ishida, Y., 1993. Preparation and characterisation of monoclonal antibodies to Alexandrium species. In: T.J. Smayda and Y. Shimizu (Editors), Toxic Phytoplankton Blooms in the Sea. Elsevier, Amsterdam, pp. 87-93.
16. Sako, Y., Murakami, T., Adachi, M., Uchida, A., Ishida, Y, Yamaguchi, M. and Takeuchi, T., 1996. Detection of the toxic dinoflagellates Alexandrium species by flow cytometry using a monoclonal antibody. In: T. Yasumoto, Y. Oshima and Y. Fukuyo (Editors), Harmful and Toxic Algal Blooms. Intergovt. Oceanogr. Comm. UNESCO, Sendai Kyodo, pp. 463-466.
17. Silva, E.S. and Faust, M.A., 1995. Small cells in the life history of dinoflagellates (Dinophyceae): a review. Phycologia, 34: 396-408.
18. Tangen, K. and Björnland, T., 1981. Observations on pigments and morphology of Gyrodinium aureolum Hulburt, a marine dinoflagellate containing 19'-hexanoyloxyfucoxantin as a main carotenoid. J. Plankton Res., 3: 380-401.
19. Taylor, J.A. and Lewis, J., 1995. Immunofluorescence of Alexandrium species from the United Kingdom. In: P. Lassus, G. Arzul, E. Erard, P. Gentien and C. Marcaillou (Editors), Harmful Marine Algal Blooms. Lavoisier, Paris, pp. 89-94.
20. Vrieling, E.G. and Anderson, D.M., 1996. Immunofluorescence in phytoplankton research: applications and potential. J. Phycol., 32:1-16.
21. Vrieling, E.G., Draaijer, A., Van Zeijl, W.J.M., Peperzak, L., Gieskes, W.W.C. and Veenhuis, M., 1993a. The effect of labelling intensity, estimated by real time confocal laser scanning microscopy, on flow cytometric appearance and identification of immunochemically labelled marine dinoflagellates. J. Phycol., 29: 180-188.
22. Vrieling, E.G., Gieskes, W.W.C., Colijn, F., Hofstraat, J.W., Peperzak, L. and Veenhuis, M., 1993b. Immunochemical identification of toxic marine algae: first results with Prorocentrum micans as model organism. In: T.J. Smayda and Y. Shimizu (Editors), Toxic Phytoplankton Blooms in the Sea. Elsevier, Amsterdam, pp. 925-931.
23. Vrieling, E.G., Peperzak, L., Gieskes, W.W.C. and Veenhuis, M., 1994. Detection of the ichthyotoxic dinoflagellate Gyrodinium (cf.) aureolum and morphologically related Gymnodinium species using monoclonal antibodies: a specific immunological tool. Mar. Ecol. Prog. Ser, 103: 165-174.
24. Vrieling, E.G., Gieskes, W.W.C., Rademaker, T.W.M., Vriezekolk, G., Peperzak, L. and Veenhuis, M., 1995. Flow cytometric identification of the ichthyotoxic dinoflagellate Gyrodinium aureolum in the central North Sea. In: P. Lassus, G. Arzul, E. Erard, P. Gentien and C. Marcaillou (Editors), Harmful Marine Algal Blooms. Lavoisier, Paris, pp. 743-748.
25. Vrieling, E.G., Vriezekolk, G., Gieskes, W.W.C., Veenhuis, M. and Harder, W., 1996. Immuno-flow cytometric identification and enumeration of the ichthyotoxic dinoflagellate Gyrodinium aureolum Hulburt in artificially mixed algal populations. J. Plankton Res., 18: 1503-1512.
26. Yamaguchi, M., 1992. DNA synthesis and the cell cycle in the noxious red-tide dinoflagellate Gymnodinium nagasakiense. Mar. Biol., 112: 191-198.