Immunofluorescence in phytoplankton research: Applications and potential

Journal of Phycology

Volumn 32, Issue 1, 1996, Pages 1-16

  Vrieling, Engel G ^a,b   Anderson, Donald M ^a  

^a UNIVERSITY OF GRONINGEN   (Netherlands)

^b WOODS HOLE OCEANOGRAPHIC INSTITUTION   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

EID: 0029787843     PISSN: 00223646     EISSN: None     Source Type: Journal    
DOI: 10.1111/j.0022-3646.1996.t01-1-00001.x     Document Type: Review

References (115)

1. Adachi, M., Sako, Y. & Ishida, Y. 1993. The identification of conspecific dinoflagellates Alexandrium tamarense from Japan and Thailand by monoclonal antibodies. ANipp. Suis. Gakk. 59: 327-32.
2. Anderson, D. M. 1995. Identification of harmful algal species using molecular probes: an emerging technology. In Lassus, P., Arzul, G., Erard, E., Gentien, P. & Marcaillou, C. [Eds.] Harmful Marine Algal Blooms. Lavoisier, Paris, pp. 3-13.
3. Anderson, D. M. & P-O Cheng, T. 1988. Intracellular localization of saxitoxins in the dinoflagellate Gonyaulax tamarensis. J. Phycol. 24:17-22.
4. Anderson, D. M., Keafer, B. A., Kulis, D. M., Waters, R. M. & Nuzzi, R. 1993. An immunofluorescent survey of the brown tide chrysophyte Aureococcus anophagefferens along the northeast coast of the United States./ Plankton Res. 15:563-80.
5. Anderson, D. M., Kulis, D. M. & Cosper, E. M. 1990. Immunofluorescent detection of the brown tide organism Aureococcus anophagefferens. In Cosper, E. M., Bricelj, V. M. & Carpenter, E. J. [Eds.] Noi'el Phytoplankton Blooms: Causes and Impacts of Recurrent Brown Tides and Other Unusual Blooms. Springer-Verlag, Berlin, pp. 213-28.
6. Baden, D. G., Mende, T. J. & Szmant, A. M. 1988. Brevetoxins and binding: sodium channels versus antibodies. In Yentsch, C. M., Mague, F. C. & Horan, P. K. [Eds.] Immunochemical Approaches to Coastal, Estuarine and Oceanicographic Questions, Lecture Notes on Coastal and Estuarine Studies, Vol. 25. Springer-Verlag, New York, pp. 134-44.
7. Baker, K. H. & Mills, A. L. 1982. Determination of the number of respiring Thiobacillus ferrooxidans cells in water samples by using combined fluorescent antibody-2-(p-iodophenyl)-3-(pnitrophenyl)-5-phenyltertazolium chloride staining. Appl. Environ. Microbiol. 43:338-44.
8. Balch, W. M., Yentsch, C. M., Reguera, B. & Campbell, W. 1988. Examining nitrate reduction by phyto-plankton with an immunoassay. In Yentsch, C. M., Mague, F. C. & Horan, P. K. [Eds.] Immunochemical Approaches to Coastal, Estuarine and Océanographie Questions, Lecture Notes on Coastal and Estuarine Studies, Vol. 25. Springer-Verlag, New York, pp. 263-76.
9. Balfoort, H. W., Snoek, J., Smits, J. R. M., Breedveld, L. W., Hofstraat, J. W. & Ringelberg, J. 1992. Automatic identification of algae: neural network analysis of flow cytometric data./ Plankton Res. 14:575-89.
10. Bates, S. S., Léger, C., Keafer, B. A. & Anderson, D. M. 1993. Discrimination between domoic-acid-producing and nontoxic forms of the diatom Pseudonitzschia pungens using immunofluorescence. Mar. Ecol. Prog. Ser. 100:185-95.
11. Bernhard, M., Lomi, G., Riparbelli, G., Saletti, M. & Zattera, A. 1969. Un metodo immunologico per la caratterizzazzione del fitoplancton. Estratto Dalle Publ. Stau. ZooJ. Xapoli 37(2 Suppl.):64-72.
12. Borman, A. H., de Vrind-de Jonge, E. W., Thierry, R., Westbroek, P. & Bosch, L. 1987. Coccolith associated polysaccharides from cells of Emiliania huxleyi (Haptophyceae). J. Phycol. 23:118-23.
13. Buma, A. G. J., van Hannen, E. J., Roza, L., Veldhuis, M. & Gieskes, W. W. C. 1995. Monitoring ultraviolet-B induced DNA damage in individual diatom cells by immunofluorescent thymine dimer detection.J. Phycol. 31:314-21.
14. Buurman, E. P., Sanders, R., Draaijer, A., Gerritsen, H. C., van Veen.J. J. F., Houpt, P. M. & Levine, Y. K. 1992. Fluorescence lifetime imaging using a confocal laser scanning microscope. Scanning 14:155-9.
15. Campbell, L. & Carpenter, E.J. 1987. Characterization of phycoerythrin-containing Synechococcus spp. populations by immunofluorescence./ Plankton Res. 9:1167-81.
16. Campbell, L., Carpenter, E. J. & lacono, V. I. 1983. Identification and enumeration of marine chroococcoid cyanobacteria by immunofluorescence. Appl. Environ. Microbiol. 46: 553-9.
17. Campbell, L. & Iturriaga, R. 1988. Identification of Synechococcus spp. in the Sargasso Sea by immunofluorescence and fluorescence excitation spectroscopy performed on individual cells. Limnol. Oceanogr. 33:1196-201.
18. Campbell, L., Shapiro, L. P. & Haugen, E. 1994. Immunochemical characterization of eukaryotic ultraplankton from the Atlantic and Pacific oceans./ Plankton Res. 16:35-51.
19. Carlson, R. E., Lever, M. L., Lee, B. W. & Guire, P. E. 1984. Development of immunoassays for paralytic shellfish poisoning: a radioimmunoassay for saxitoxin. In Ragelis, E. P. [Ed.] Seafood Toxins. American Chemical Society, Washington, D.C., pp. 181-92.
20. Cembella, A. D., Sullivan, J. J., Boyer, G. L., Taylor, F. J. R. & Anderson, R. J. 1987. Variation in paralytic shellfish toxin composition within the Protogonyaulax tamarensis/catenella species complex: red tide dinoflagellates. Biochem. Syst. Ecol. 15:171-86.
21. Cembella, A. D., Therriault, J. C. & Beland, P. 1988. Toxicity of cultured isolates and natural populations of Prologonyaulax lamarensis (Labour) Taylor from the St. Lawrence estuary. J. Shellfish Res. 7:611-22.
22. Chisholm, S. W., Olson, R. J., Zeltler, E. R., Goericke, R., Waterbury, J. B. & Welsch-meyer, N. A. 1988. A novel freeliving prochlorophyte abundant in the oceanic euphotic zone. Mature (Land.) 334:340-3.
23. Coleman, A. W. 1988. The autofluorescent flagellum: A new phylogenetic enigma./ Phycol. 24:118-20.
24. Coons, A. H., Creech,J. H. &Jones, N. R. 1941. Immunological properties of an antibody containing a fluorescent group. Proc. Soc. Exp. Biol. Med. 47:200.
25. Coons, A. H., Leduc, E. H. & Connolly, J. M. 1955. Studies of antibody production. I: A method for the histochemical demonstration of specific antibody and its application to a study of the hyperimmune rabbit.J. Exp. Med. 102:49-60.
26. Cosper, E. M., Bricelj, V. M. & Carpenter, E. J., Eds. 1989. Novel Phytoplankton Blooms: Causes and Effects of Recurrent Brown Tides and Other Unusual Blooms. Springer-Verlag, Berlin, 799 pp.
27. Cunningham, A. 1990. A low-cost, portable flow cytometer specifically designed for phytoplankton analysis./ Plankton Res. 12:149-60.
28. 2-fixing microorganisms from aquatic environments. Limnol. Oceanogr. 35:59-71.
29. Dahl, A. B. & Laake, M. 1982. Diversity dynamics of marine bacteria studied by immunofluorescent staining on membrane filters. Appl. Environ. Microbiol. 43:169-76.
30. Domozych, D. S. &: Rogers Domozych, C. 1993. Mucilage processing and secretion in the green alga Closlerium. II. Ultrastructure and immunocytochemistry.y. Phycol. 29:659-67.
31. Doucette, G. J. & Anderson, D. M. 1993. Intracellular distribution of saxitoxin in Alexandrium fundyense. In Smayda, T. J. & Shimizu, Y. [Eds.] Toxic Phytoplankton Blooms in the Sea. Elsevier, Amsterdam, pp. 863-8.
32. Dovichi, N.J., Martin, J. C., Jett, J. H. & Keller, R. A. 1983. Attogram detection limit for aqueous dye samples by laserinduced fluorescence. Science (\Vash. D.C.) 219:845-7.
33. Dubelaar, G. B. J., Groenewegen, A. D., Stokdijk, W., van den Engh, G. J. & Visser.J. W. M. 1989. Optical Plankton Analyzer: a flow cytometer for plankton analysis, II: Specifications. Cylometry 10:529-39.
34. Eardly, D. D., Sutton, C. W., Hempel, W. M., Reed, D. C. & Ebeling, A. W. 1990. Monoclonal antibodies specific for sulfated polysaccharides on the surface of Macrocystis pyrifera (Phaeophyta).J. Phycol. 26:54-62.
35. Fliermans, C. B., Bohlool, B. B. & Schmidt, E. L. 1974. Autecological study of the chemoautotroph Nitrobacter by immunofluorescence. Appl. Microbiol. 27:124-9.
36. Fliermans, C. B. & Schmidt, E. L. 1977. Immunofluorescence for autecological study of a unicellular bluegreen alga. J. Phycol. 13:364-8.
37. Gates, J.E.&Pham.K. D. 1979. An indirect fluorescent antibody staining technique for determining population levels of Thiobacillus ferrooxidans in acid mine drainage waters. MicrobialEcol. 5:121-7.
38. Giloh, H.&Sedat,J. W. 1982. Fluorescence microscopy: reduced photobleaching of rhodamine and fluorescein protein conjugates by n-propylgallate. Science (Wash. D.C.) 217:1252-4.
39. Glover, H.E., Campbell, L. & Prézelin, B. B. 1986. Contribution of Synechococcus spp. to size-fractioned primary production in three water masses in the Northwest Atlantic Ocean. Mar. Biol. (Berl.) 91:193-203.
40. Griffiths, G., Parton, R. G., Lucocq, J., van Deurs, B., Brown, D, Slot, J. W. & Geuze, H. J. 1993. The immunofluorescent era of membrane traffic. Trends Cell Biol. 3:214-9.
41. Guire, P. E., Duquette, P. H., Amos, R. A., Behrens, M. W., Josephson, M. W. & Chambers, R. P. 1988. Preparation of model haptens to express common epitopes on PSP toxins. In Yentsch, C. M., Mague, F. C. & Horan, P. K. [Eds.] Immunochemical Aprroaches to Coastal, Estuarine and Oceania-graphie Questions, Lecture Notes on Coastal and Estuarine Studies, Vol. 25. Springer-Verlag, New York, pp. 145-54.
42. Harlow, E. & Lane, D., Eds. 1988. Antibodies: A Laboratory Manual. CSH Laboratory, New York, 726 pp.
43. Haugland, R. P., Ed. 1989. Handbook of Fluorescent Probes and Research Chemicals. Catalogue of Molecular Probes, Eugene, Oregon, 421 pp.
44. Hiroishi, S., Uchida, A., Nagasaki, K. & Ishida, Y. 1988. A new method for identification of inter- and intra-species of the red tide algae Chattonella antiqua and Chattonella marina (Raphidophyceae) by means of monoclonal antibodies.J. Phycol. 24:442-4.
45. Hofstraat.J. W., van Zeijl, W. J. M., Peelers, J. C. H., Peperzak, L. & Dubelaar, G. B. J. 1990. Flow cytometry and other optical methods for characterization and quantification of phytoplankton in seawater. In Nielsen, H. O. [Ed.] Environment and Pollution Measurement Sensors and Systems, SPIE Proceedings, Vol. 1269. Bellingham, Washington, pp. 116-33.
46. Hokama, Y., Honda, S. A. A., Kobayashi, M. N., Nakagawa, L. K., Shiria, L. K. & Miyahara, J. T. 1988. Monoclonal antibodies (Monoabs) to ciguatoxin and related polyethers. In Yentsch, C. M., Mague, F. C. & Horan, P. K. [Eds.] Immunochemical Approaches to Coastal, Estuarine and Oceanicographic Questions, Lecture Notes on Coastal and Estuarine Studies, Vol. 25. Springer-Verlag, New York, pp. 155-66.
47. Homan, W. L., van Kalshoven, H., Kolk, A. H. J., Musgrave, A., Schuring, F. & van den Ende, H. 1987. Monoclonal antibodies to surface glycoconjugates in Chlamydomonas eugametos recognize strain-specific O-methyl sugars. Planta (Berl.) 170: 328-35.
48. Hudson, L. & Hay, F. C., Eds. 1989. Practical Immunolgy (3rd ed.). Blackwell, London, 507 pp.
49. Jeffris, R. & Deverill, I. 1991. The antigen antibody reaction. /)i Price, C. P. & Newman, D. J. [Eds.] Principles and Practice of Immunochemistry. Stocton Press, New York, pp. 1-18.
50. Johnson, G. D. & Nogueira Araujo, G. M. de C. 1981. A simple method of reducing the fading of immunofluorescence during microscopy.J. Immunol. Methods 43:349-50.
51. Josserand, A. &Cleyet-Marel,J. C. 1979. Isolation from soils of Nitrobacter and evidence for novel serotypes using immunofluorescence. Microbial Ecol. 5:197-205.
52. Kajikawa, H., Okada, M., Ishikawa, F., Okada, Y. & Nakayama, K. 1988. Immunochemical studies on ribulose 1,5-bisphosphate carboxylase/oxygenase in the chloroplasts of the marine alga Bryopsis maxima. Plant Cell Physiol. 29:549-56.
53. Kasten, F. H. 1989. The origins of modern fluorescence microscopy and fluorescent probes. In Kohen, E. & Hirschberg, J. G. [Eds.] Cell Structure and Function by Microspectroßuorometry. Academic Press, New York, pp. 3-50.
54. Kawai, H. 1988. A flavin-like autofluorescent substance in the posterior flagellum of golden brown algae.J. Phycol. 24:114-7.
55. Kawai, H.&Inouye, I. 1989. Flagellar autofluorescence in fortyfour chlorophyll c-containing algae. Phycologia 28:222-7.
56. Langanger, G., deMey,J. & Adam, M. 1983. 1,4-Diazobizyklo[2,2,2]-oktan (DABCO) verzögert das Ausbleichen von immunofluoreszenz Präparaten. Mikroskopie (\Vien) 40:237-41.
57. Lee, J.J., Chan, Y. & Lagziel, A. 1988. An immunofluorescence approach toward the identification of endosymbiotic diatoms in several spieces of larger Foraminifera. In Yentsch, C. M., Mague, F. C. & Horan, P. K. [Eds.] Immunochemical Approaches in Coastal, Estuarine and Océanographie Questions, Lecture Notes on Coastal and Estuarine Studies, Vol. 25. Springer-Verlag, New York, pp. 230-41.
58. Legendre, L.& Yentsch, C. M. 1989. Overview of flow cytometry image analysis in biological oceanography and limnology. Cytometry 10:501-10.
59. Lin, S., Chang.J. & Carpenter, E.J. 1994. Detection of proliferating cell nuclear antigen analog in four species of marine phytoplankton.J. Phycol. 30:449-56.
60. - 1995. Growth characteristics of phytoplankton determined by cell cycle proteins: PCNA immunostaining of Dunaliella terliolecta (Chlorophyceae).J. Phycol. 31:388-95.
61. Melkonian, M., Ed. 1992. Algal Cell Motility, Current Phycology 3. Chapman & Hall, London, 236 pp.
62. Melkonian, M., Schulze, D., McFadden, G. I. & Robenek, H. 1988. A polyclonal antibody (anticentrin) distinguishes between two types of fibrous flagellar roots in green algae. Protoplasma 144:56-61.
63. Mendoza, H., Lôpez-Rodas, V., Gonzâlez-Gil, S., Aguilera, A. & Costas, E. 1995. Use of polyclonal antisera and blocking of the antibodies in the identification of marine dinoflagellates: species-specific and clone-specific antisera against Gymnodinium and Alexandrium. J. Exp. Mar. Biol. Ecol. 186:103-15.
64. Menzel, D., Ed. 1992. The Cytoskeleton of the Algae. CRC Press, London, 453 pp.
65. Motomura, T. 1991. Immunofluorescence microscopy of fer_tilization and parthenogenesis in Laminaria angustata (Phaéophyta).y. Phycol. 27:248-57.
66. Müller, D. G., Maier, I. & Müller, H. 1987. Flagellum autofluorescence and photoaccumulation in heterokont algae. Photochem. Photobiol. 46:1003-8.
67. Musgrave, A., de Wildt, P., Broekman, R. & van den Ende, H. 1983. The cell wall of Chlamydomonas eugametos. Immunological aspects. Planta (Berl.) 158:82-9.
68. Musgrave, A., van Eijk, F., te Welcher, R., Broekman, R., Lens, P. F., Homan, W. L. & van den Ende, H. 1981. Sexual agglutination factor from Chlamydomonas eugametos. Planta (Berl.) 153:362-9.
69. Nagasaki, K., Uchida, A. & Ishida, Y. 1991. A monoclonal antibody which recognizes the cell surface of red tide alga Gymnodinium nagasakiense. Nipp. Suis. Gakk. 57:1211-24.
70. Oison, R. J., Chisholm, S. W., Zeltler, E. R. & Armbrust, E. V. 1988. Analysis of Synechococcus pigment types in the sea using single and double beam flow cytometry. Deep Sea Res. 35: 425-40.
71. Olson, R. J., Vaulot, D. & Chisholm, S. W. 1985. Marine phytoplankton distributions measured using a shipboard flow cytometry. Deep Sea Res. 32:1273-80.
72. Orellana, M. V. & Perry, M. J. 1992. An immunoprobe to measure Rubisco concentrations and maximal photosynthetic rates of individual phytoplankton cells. Limnol. Oceanogr. 37:47890.
73. _ 1995. Optimization of an immunofluorescent assay of the internal enzyme, ribulose-1,5-biphosphate carboxylase (Rubisco), in single phytoplankton cells./ Phycol. 31:785-94.
74. Orellana, M. V., Perry, M. J. & Watson, B. A. 1988. Probes for assessing single-cell primary production: antibodies against ribulose-l ,5-bisphosphate carboxylase (RuBPCASE) and peridinin/chlorophyll A protein (PCP). In Yentsch, C. M., Mague, F. C. & Horan, P. K. [Eds.] Immunochemical Approaches to Coastal, Estuarine and Océanographie Questions, Lecture Notes on Coastal and Estuarine Studies, Vol 25. Springer-Verlag, New York, pp. 243-62.
75. Peeters.J. C. H., Dubelaar, G. B. J., Ringelberg, J. & Visser, J. W. M. 1989. Optical Plankton Analyzer: a flow cytometer for plankton analysis, I: Design considerations. Cytometry 10: 522-8.
76. Phinney, D. A. & Cucci, T. L. 1989. Flow cytometry and phytoplankton. Cytometry 10:511-21.
77. Ploem, J. S. 1989. Fluorescence scanning instrumentation. In Kohen, E. & Hirschberg, J. G. [Eds.] Cell Structure and Function byMicrospectroßuorometry. Academic Press, New York, pp. 119-30.
78. Pomeroy, L. R. & Johannes, R. E. 1968. Occurrence and respiration of ultraplankton in the upper 500 meters of the ocean. Deep Sea Res. 15:381-91.
79. Roberts, K. R. 1991. The flagellar apparatus and cytoskeleton of dinoflagellates: organization and use in systematics. In Patterson, D. J. & Larsen.J. [Eds.] The Biology of Free-Living Flagellates. Systematics Association Special Volume No. 45. Clarendon Press, Oxford, pp. 285-302.
80. Roberts, K. R., Lemoine, J. E., Schneider, R. M. & Farmer, M. A. 1988. The microtubular cytoskeleton of three dinoflagellates: an immunofluorescence study. Protoplasma 144:6871.
81. Roberts, K. R., Losoff Rusche, M. & Taylor, F. J. R. 1993. The cortical microtubular cytoskeleton of Oxyrrhis marina (Dinophyceae) observed with immunofluorescence and electron microscopy./ Phycol. 29:642-9.
82. Roberts, K. R. & Roberts, J. E. 1991. The fiagellar apparatus and cytoskeleton of the dinoflagellates: a comparative over\\ew.Protoplasma 164:105-22.
83. Rogers Domozych, C., Plante, K., Biais, P., Paliulis, L. & Domozych, D. S. 1993. Mucilage processing and secretion in the green alga Closterium. I. Cytology and biochemistry./ Phycol. 29:650-9.
84. Sako, Y., Adachi, M. & Ishida, Y. 1993. Preparation and characterization of monoclonal antibodies to Alexandrium species. In Smayda, T. J. & Shimizu, Y. [Eds.] Toxic Phytoplankton Blooms in the Sea. Elsevier, Amsterdam, pp. 87-93.
85. 2+-modulated contractile protein in the flagellar apparatus of green algae: the nucleusbasal connector. Eur.J. Cell Biol. 45:51-61.
86. Segaar, P. J. & Lokhorst, G. M. 1987. Cell division in the green alga Ulothrix palusaha (Ulvophyceae, Chlorophyta): a combined immunofluorescence and transmission electron microscopy study. Phycologia 26:100-10.
87. Shapiro, H. M., Ed. 1985. Practical Flow Cytometry. Alan R. Liss, New York, 353 pp.
88. Shapiro, L. P., Campbell, L. & Haugen, E. M. 1989a. Immunochemical recognition of phytoplankton species. Mar. Ecol. Prog.Ser. 57:219-24.
89. Shapiro, L. P., Haugen, E. M. & Carpenter, E. J. 1989b. Occurrence and abundance of green-fluorescing dinoflagellates in surface waters of the Northwest Atlantic and Northeast Pacific oceans./ Phycol. 25:189-91.
90. Shapiro, L. P., Haugen, E. M., Keller, M. D., Bidigare, R. R., Campbell, L. & Guillard, R. R. L. 1989c. Taxonomic affinities of marine coccoid ultraphytoplankton: a comparison of immunochemical surface antigen cross-reactions and HPLC chloroplast pigment signatures./ Phycol. 25:794-7.
91. Smith, E., Roberts, K., Hutchings, A. & Galfre, G. 1984. Monoclonal antibodies to the major structural glycoprotein of the Chlamydomonas cell wall. Planta (Berl.) 161:330-8.
92. Smith, J. C., Odense, P., Angus, R., Bates, S. S., Bird, C. J., Cormier, P., de Freitas, A. S. W., Léger, C., O'Neil, D., Pauley, K. & Worms, J. 1990. Variation in domoic acid levels in Xitzschia species: implications for monitoring programs. Bull. Aquamlt. Assoc. Can. 90:27-31.
93. Steen H. B. 1991. Flow cytometry instrumentation. In Demers, S. [Ed.] Particle Analysis in Oceanography, NATO/ASI Series, Series G: Ecological Sciences, Vol. 27. Springer-Verlag, Berlin, pp. 3-29.
94. Taylor, J. A. &: Lewis, J. 1995. Immunofluorescence of Alexandrium species from the United Kingdom. In Lassus, P., Arzul, G., Erard, E., Gentien, P. & Marcaillou, C. [Eds.] Harmful Marine Algal Blooms. Lavoisier Intercept, Paris, pp. 89-94.
95. Tsuji.T. &Yaganati,T. 1981. Improved fluorescent microscopy for measuring the standing stock of phytoplankton including fragile components. Mar. Biol. (Berl.) 64:207-11.
96. Uchida, A., Nagasaki, K., Hiroishi, S. & Ishida, Y. 1989. The application of monoclonal antibodies to an identification of Chattonella marina and Chattonella antiqua. Nipp. Suis. Gakk. 55:721-5.
97. van Bleijswijk, J., van der Wal, P., Kempers, R., Veldhuis, M., Young, J. R., Muyzer, G de Vrind-dejong, E. & Westbroek, P. 1991. Distribution of two types otEmiliania huxleyi (Prymnesiophyceae) in the Northeast Atlantic region as determined by immunofluorescence and coccolith morphology. J Phycol. 27:566-70.
98. Visser, J., Haaijman, J. & Trask, B. 1978. Quantitative immunofluorescence in flow cytometry. In Knapp, W., Holubar, K. & Wick, G. [Eds.] Immunoßuorescence and Related Stainings Techniques. Eisevier, Amsterdam, pp. 147-60.
99. Vladimirova, M. G., Markelova, A. G. & Semenenko, V. E. 1982. Use of the cytoimmunofluorescent method to clarify localization of ribulose bisphosphate carboxylase in pyrenoids of unicellular algae. Fiziol. Rast. 29:941-50.
100. Voigt.J. 1988. The lithium-chloride-soluble cell-wall layers of Chlamydomonas reinhardii contain several immunologically related glycoproteins. Planta (Berl.) 173:373-84.
101. Vrieling, E. G., Draaijer, A., van Zeijl, W. J. M., Peperzak, L., Gieskes, W. W. C. & Veenhuis, M. 1993a. The effect of labelling intensity, estimated by real time confocal laser scanning microscopy, on flow cytometric appearance and identification of immunochemically labelled marine dinoflagellate species./ Phycol. 29:180-8.
102. Vrieling, E. G., Gieskes, W. W. C., Colijn, F., Hofstraat, J. W., Peperzak, L.& Veenhuis, M. 1993b. Immunochemical identification of toxic marine algae: first results with Prorocentrum micans as model organism. In Smayda, T. J. & Shimizu, Y. [Eds.] Toxic Marine Phytoplankton Blooms in the Sea. Elsevier, Amsterdam, pp. 925-31.
103. Vrieling, E. G., Gieskes, W. W. C., Rademaker, T. W. M., Vriezekolk, G., Peperzak, L. & Veenhuis, M. 1995a. Flow cytometric identification of the ichthyotoxic dinoflagellate Gyrodinium aureolum in the central North Sea. In Lassus, P., Arzul, G., Erard, E., Gentien, P. & Marcaillou, C. [Eds.] Harmful Marine Algal Blooms. Lavoisier, Paris, pp. 743-8.
104. Vrieling, E. G., Gieskes, W. W. C., van Zeijl, W. J. M. & Veenhuis, M. 1994a. Detection of Gyrodinium aureolum in Dutch coastal waters by particle analysis based on immuno-flow cytometry. NetLJ. Aquat. Ecol 28:249-54.
105. Vrieling, E. G., Koeman, R. P. T., Peperzak, L., Nagasaki, K. & Ishida, Y. 1995b. Chattonella and Fibrocapsa (Raphidophyceae): first observation of potentially harmful, red tide organisms in the Dutch Wadden Sea. Neth.J. Sea Res. 33:183-91.
106. Vrieling, E. G., Peperzak, L., Gieskes, W. W. C. & Veenhuis, M. 1994b. Detection of the ichthyotoxic dinoflagellate Gyrodinium cf. aureolum and morphologically related Gymnodinium species using monoclonal antibodies: a specific immunological tool. Mar. Ecol. Prog. Ser. 103:165-74.
107. Ward, B. B. 1990. Immunology in biological oceanography and marine ecology. Oceanography 3:30-5.
108. Ward, B. B. & Carlucci, F. 1985. Marine ammonia-and nitriteoxidizing bacteria: serological diversity determined by immunofluorescence in culture and in the environment. Appl. Env. Microbiol. 50:194-201.
109. Ward, B. B. & Perry, M.J. 1980. Immunofluorescent assay for the marine ammonium-oxidizing bacterium Nitrococcus oceanus. Appl. Env. Microbiol. 39:913-8.
110. Williams, A. F. & Woollet, G. R. 1985. What monoclonals see at cell surfaces. Biochem. Soc. Trans. 13:1-3.
111. Wilson, T. 1990, Ed. Confocal Microscopy. Academic Press, London, 426 pp.
112. Yentsch, C. M., Dale, B. & Hurst, J. W. 1978. Coexistence of toxic and non-toxic dinoflagellates resembling Gonyaulax tamarensis in New England coastal waters (N. W. Atlantic). J. Phycol. 14:330-2.
113. Yoshioka, T., Terai, H.&Saijo, Y. 1982. Growth kinetic studies of nitrifying bacteria using immunofluorescent counting method./ Gen. Appl. Microbiol. 28:169-80.
114. Zhang, Y. &Wu,M. 1993. Fluorescence microscopy on dynamics changes of frx B distribution in Chtamydomonas reinhardii. Protoplasma 172:57-63.
115. Zhou, J. & Fritz, L. 1994. Okadaic acid antibody localizes to chloroplasts in the DSP-toxin-producing dinoflagellates Prorocentrum lima and Prorocentrum maculosum. Phycologia 33:45561.