Novel mutant green fluorescent protein protease substrates reveal the activation of specific caspases during apoptosis

Chemistry and Biology

Volumn 6, Issue 6, 1999, Pages 401-409

  Mahajan, Nupam P ^a   Harrison Shostak, D Corinne ^a   Michaux, Jennifer ^a   Herman, Brian ^a,b  

^a UNIVERSITY OF NORTH CAROLINA   (United States)

^b UNIVERSITY OF TEXAS HEALTH SCIENCE CENTER AT SAN ANTONIO   (United States)

Author keywords

Apoptosis; Caspase; Green fluorescent protein (GFP)

Indexed keywords

ANIMALIA; MAMMALIA;

EID: 0033150406     PISSN: 10745521     EISSN: None     Source Type: Journal    
DOI: 10.1016/S1074-5521(99)80051-9     Document Type: Article

References (18)

1. Ellis, H.M. & Horvitz, H.R. (1986). Genetic control of programmed cell death in nematode C. Elegans. Cell 44, 817-829.
2. Yuan, J., Shaham, S., Ledoux, S., Ellis, H.M. & Horvitz, H.R. (1993). The C. Elegans cell death gene ced-3 encodes a protein similar to mammalian interleukin-1 β-converting enzyme. Cell 75, 641-652.
3. Alnemeri, E.S., et al., & Yuan, J.Y. (1996). Human ICE/CED-3 protease nomenclature. Cell 87, 171.
4. Howard, A.D., et al., & Tocci, M.J. (1991). IL-1 β-converting enzyme requires aspartic acid residues for processing of the IL-1 β precursor at two distinct sites and does not cleave 31-kDa IL-1 β. J. Immunol. 147, 2964-2969.
5. Martin, S.J. & Green, D.R. (1995). Protease activation during apoptosis: Death by a thousand cuts? Cell 82, 349-352.
6. Kumar, S. & Harvey, NL. (1995). Role of multiple cellular protease in the execution of programmed cell death. FEBS Lett. 375, 169-173.
7. Tewari, M., et al., & Dixit, V.M. (1995). Yama/CPP32b, a mammalian homolog of CED-3 is a CrmA-inhibitable protease that cleaves the death substrate poly(ADP-ribose) polymerase. Cell 81, 801-809.
8. Enari, M., Sakahira, H., Yokoyama, H., Okawa, K., Iwamatsu, A. & Nagata, S. (1998). A caspase-activated DNase that degrade DNA during apoptosis, and its inhibitor ICAD. Nature 391, 43-50.
9. Nicholson, D.W., et al., & Miller, D.K. (1995). Identification and inhibition of the ICE/Ced-3 protease necessary for mammalian apoptosis. Nature 376, 37-43.
10. Takahashi, A., et al., & Earnshaw, W.C. (1996). Cleavage of lamin A by Mch2a but not CPP32: Multiple interleukin 1 b-converting enzyme-related proteases with distinct substrate recognition properties are active in apoptosis. Proc. Natl Acad. Sci. USA 93, 8395-8400.
11. Mancini, M., et al., & Rosen, A. (1998). The caspase-3 precursor has a cytosolic and mitochondrial distribution: Implications for apoptotic signaling. J. Cell. Biol. 140, 1485-1495.
12. Mahajan, N.P., Linder, K., Berry, G., Gordon, G.W., Heim, R. & Herman, B. (1998). Bcl-2 and Bax interactions in mitochondria probed with green fluorescent protein and fluorescence resonance energy transfer. Nat. Biotechnol. 16, 547-552.
13. Cubitt, A.B., Heim, R., Adams, S.R., Boyd, A.E., Gross, L.A. & Tsien, R.Y. (1995) Understanding, improving and using green fluorescent proteins. Trends Bio. Sci. 20, 448-455.
14. Rizzuto, R., et al., & Pozzan, T. (1996). Double labeling of subcellular structures with organelle-targeted GFP mutants in vivo. Curr. Biol. 6, 183-188.
15. Sakai, N., Sasaki, K., Ikegaki, N., Shirai, Y., Ono, Y. & Saito, N. (1997). Direct visualization of the g-subspecies of protein kinase C in living cells using fusion proteins with green fluorescent protein. J. Cell Biol. 139, 1465-1476.
16. 2+ based on green fluorescent proteins and calmodulin. Nature 388, 882-887.
17. Heim, R. & Tsien, R.Y. (1996). Engineering green fluorescent protein for improved brightness, longer wavelengths and fluorescence resonance energy transfer. Curr. Biol. 6, 178-182.
18. Jacobson, M.D., Weil, M. & Raff, M.C. (1996). Role of Ced-3/ICE-family proteases in staurosporine-induced programmed cell death. J. Cell Biol. 133, 1041-1051.