Antisense oligonucleotide experiments elucidate the essential role of titin in sarcomerogenesis in adult rat cardiomyocytes in long-term culture

Journal of Cell Science

Volumn 113, Issue 21, 2000, Pages 3851-3859

  Person, V ^a   Kostin, S ^a   Suzuki, K ^a   Labeit, S ^a   Schaper, J ^a  

^a MAX PLANCK INSTITUTE FOR HEART AND LUNG RESEARCH   (Germany)

Author keywords

Cardiomyocyte; Oligonucleotide; Sarcomerogenesis; Titin

Indexed keywords

ALPHA ACTININ; ANTISENSE OLIGONUCLEOTIDE; CONNECTIN; FLUORESCEIN; MESSENGER RNA; MYOSIN; OLIGODEOXYNUCLEOTIDE PHOSPHOROTHIOATE;

ANIMAL CELL; ANIMAL TISSUE; ARTICLE; CELL CULTURE; CELL DIFFERENTIATION; CELL SHAPE; CELL SIZE; CELL SURFACE; CONTROLLED STUDY; HEART MUSCLE CELL; HYPOTHESIS; LABELING INDEX; LASER MICROSCOPY; MOLECULAR INTERACTION; MUSCLE DEVELOPMENT; MYOFILAMENT; NONHUMAN; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN EXPRESSION; PROTEIN PROCESSING; RAT; SARCOMERE;

ANIMALS; BASE SEQUENCE; CELLS, CULTURED; DNA PRIMERS; HEART; MUSCLE PROTEINS; MYOCARDIUM; OLIGONUCLEOTIDES, ANTISENSE; PROTEIN KINASES; RATS; RATS, WISTAR; RNA, MESSENGER; SARCOMERES;

ANIMALIA;

EID: 0033677831     PISSN: 00219533     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (46)

1. Cellular uptake and intracellular fate of antisense oligonucleotides
2. Titin domain patterns correlate with the axial disposition of myosin at the end of the thick filament
3. Synthetic oligonucleotides: Useful molecules? A review
4. Culture of the terminally differentiated adult cardiac muscle cell: A light and scanning electron microscope study
5. Myofibrillogenesis in the developing chicken heart: Assembly of Z-disk, M-line and the thick filaments
6. Cellular titin localization in stress fibers and interaction with myosin II filaments in vitro
7. Myofibril formation in longterm-cultures of adult rat heart cells
8. Immunocytochemical analysis of the regeneration of myofibrils in long-term cultures of adult cardiomyocytes of the rat
9. Series of exon-skipping events in the elastic spring region of titin as the structural basis for myofibrillar elastic diversity
10. The elastic filament system in myogenesis
11. Titin, a huge, elastic sarcomeric protein with a probable role in morphogenesis
12. Myogenesis in the mouse embryo: Differential onset of expression of myogenic proteins and the involvement of titin in myofibril assembly
13. Muscle assembly: A titanic achievement?
14. Altered expression of titin and contractile proteins in failing human myocardium
15. Mechanically driven contour-length adjustment in rat cardiac titin's unique N2B sequence: Titin is an adjustable spring
16. Independent assembly of 1.6 microns long bipolar MHC filaments and I-Z-I bodies
17. Passive force generation and titin isoforms in mammalian skeletal muscle
18. Translational control of gene expression
19. Cloned rat cardiac titin class I and class II motifs. Expression, purification, characterization, and interaction with F-actin
20. Dynamics of actin and assembly of connectin (titin) during myofibrillogenesis in embryonic chick cardiac muscle cells in vitro
21. Towards a molecular understanding of titin
22. Titins: Giant proteins in charge of muscle ultrastructure and elasticity
23. The giant protein titin. Emerging roles in physiology and pathophysiology
24. Characterizing titin's I-band Ig domain region as an entropic spring
25. Characterization of oligonucleotide transport into living cells
26. Human autoantibodies reveal titin as a chromosomal protein
27. Connectin, an elastic protein of muscle. Comparative Biochemistry
28. Cultured adult cardiac myocytes: Future applications, culture methods, morphological and electrophysiological properties
29. Titin, myosin light chains and C-protein in the developing and failing human heart
30. Remodelling of adult cardiac muscle cells in culture: Dynamic process of disorganization and reorganization of myofibrils
31. Molecular structure of the sarcomeric M band: Mapping of titin and myosin binding domains in myomesin and the identification of a potential regulatory phosphorylation site in myomesin
32. Antisense experiments elucidate the essential role of titin in sarcomerogenesis
33. Oligonucleotide-mediated modulation of mammalian gene expression
34. Various hypertrophic stimuli induce distinct phenotypes in cardiomyocytes
35. Differential distribution of subsets of myofibrillar proteins in cardiac nonstriated and striated myofibrils
36. Modified deoxyoligonucleotides stable to exonuclease degradation in serum
37. Vinculin is an essential component for normal myofibrillar arrangement in fetal mouse cardiac myocytes
38. Phosphorothioate antisense oligodeoxynucleotides: Questions of specificity
39. 2+ exchanger on calcium dynamics in cultured cardiac myocytes
40. Immunocytochemical studies of cardiac myofibrillogenesis in early chick embryos. I. Presence of immunofluorescent titin spots in premyofibril stages
41. Titin: A molecular control freak
42. An N-terminal fragment of titin coupled to green fluorescent protein localizes to the Z-bands in living muscle cells: Overexpression leads to myofibril disassembly
43. Titin: Major myofibrillar components of striated muscle
44. Studies on cardiac myofibrillogenesis with antibodies to titin, actin, tropomyosin, and myosin
45. Does titin regulate the length of muscle thick filaments?
46. Quantitative determination of myosin and actin in rabbit skeletal muscle