Molecular structure of the sarcomeric M band: Mapping of titin and myosin binding domains in myomesin and the identification of a potential regulatory phosphorylation site in myomesin

EMBO Journal

Volumn 16, Issue 2, 1997, Pages 211-220

  Obermann, Wolfgang M J ^a   Gautel, Mathias ^b   Weber, Klaus ^a   Fürst, Dieter O ^a,c  

^a MAX PLANCK INSTITUTE FOR BIOPHYSICAL CHEMISTRY   (Germany)

^b EUROPEAN MOLECULAR BIOLOGY LABORATORY   (Germany)

^c UNIVERSITY OF POTSDAM   (Germany)

Author keywords

Myomesin; Myosin binding; Phosphorylation; Sarcomere; Titin binding

Indexed keywords

CONNECTIN; CYCLIC AMP DEPENDENT PROTEIN KINASE; CYTOSKELETON PROTEIN; IMMUNOGLOBULIN; MUSCLE PROTEIN; MYOMESIN; MYOSIN;

AMINO TERMINAL SEQUENCE; ARTICLE; BINDING SITE; CARBOXY TERMINAL SEQUENCE; CHEMICAL STRUCTURE; CONTROLLED STUDY; NONHUMAN; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; PROTEIN BINDING; PROTEIN DOMAIN; PROTEIN PHOSPHORYLATION; PROTEIN PROTEIN INTERACTION; SARCOMERE; SKELETAL MUSCLE;

ALANINE; ANIMALS; BACTERIOPHAGE LAMBDA; BINDING SITES; CATTLE; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ESCHERICHIA COLI; HUMANS; MEMBRANE PROTEINS; MUSCLE PROTEINS; MUSCLE, SKELETAL; MUTAGENESIS, SITE-DIRECTED; MYOSINS; PHOSPHORYLATION; POLYMERASE CHAIN REACTION; PROTEIN KINASES; RABBITS; RECOMBINANT PROTEINS; SARCOMERES; SERINE;

EID: 0031034775     PISSN: 02614189     EISSN: None     Source Type: Journal    
DOI: 10.1093/emboj/16.2.211     Document Type: Article

References (26)

1. Ausubel, F.M., Brent, R., Kingston, R.E., Moore, D.D., Seidman, J.G., Smith, J.A. and Struhl, K. (1987) Current Protocols in Molecular Biology. Wiley and Sons, Inc., New York
2. Casnellie, J.E. (1991) Assay of protein kinases using peptides with basic residues for phosphocellulose binding. Methods Enzymol., 200, 115-120.
3. Freiburg, A. and Gautel, M. (1996) A molecular map of the interactions of titin and myosin binding protein C: implications for sarcomeric assembly in familial hypertrophic cardiomyopathy. Eur. J. Biochem., 235, 317-323.
4. Fürst, D.O. and Gautel, M. (1995) The anatomy of a molecular giant: how the sarcomere cytoskeleton is assembled from immunoglobulin superfamily molecules. J. Mol. Cell. Cardiol., 27, 951-960.
5. Fürst, D.O., Osborn, M., Nave, R. and Weber, K. (1988) The organization of titin filaments in the half sarcomere revealed by monoclonal antibodies in immunoelectron microscopy: a map of 10 non repetitive epitopes starting at the Z-line extends close to the M-line. J. Cell Biol., 106, 1593-1605.
6. Gautel, M., Leonard, K. and Labeit, S. (1993) Phosphorylation of KSP motifs in the C-terminal region of titin in differentiating myoblasts. EMBO J., 12, 3827-3834.
7. Grove, B.K., Kurer, V., Lehner, C., Doetschmann, T.C. Perriard, J.-C. and Eppenberger, H. (1984) A new 185,000-dalton skeletal muscle protein detected by monoclonal antibodies. J. Cell Biol., 98, 518-524.
8. Grove, B.K., Cerny, L., Perriard, J.-C. and Eppenberger, H.M. (1985) Myomesin and M-protein: expression of two M-band proteins in pectoral muscle and heart during development. J. Cell Biol., 101, 1413-1421.
9. Grove, B.K., Holmbom, B. and Thornell, L.-E. (1987) Myomesin and M protein: differential expression in embryonic fibers during pectoral muscle development. Differentiation, 34, 106-114.
10. Kemp, B.E., Graves, D.J., Benjamini, E. and Krebs, E.G. (1977) Role of multiple basic residues in determining the substrate specifity of cyclic AMP-dependent protein kinase. J. Biol. Chem., 251, 4888-4894.
11. Laemmli, U.K. (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, 227, 680-685.
12. Maeda, K., Sczakiel, G. and Wittinghofer, A. (1987) Characterization of cDNA coding for the complete light meromyosin portion of a rabbit fast skeletal muscle myosin heavy chain. Eur. J. Biochem., 167, 97-102.
13. Margossian, S.S. and Lowey, S. (1982) Preparation of myosin and its subfragments from rabbit skeletal muscle. Methods Enzymol., 85, 55-71.
14. Masaki, T. and Takaiti, O. (1974) M-protein. J. Biochem., 75, 367-380.
15. Nave, R., Fürst, D.O. and Weber, K. (1989) Visualization of the polarity of isolated titin molecules: a single globular head on a long thin rod as the M-band anchoring domain? J. Cell Biol., 109, 2177-2188.
16. Obermann, W., Plessmann, U., Weber, K. and Fürst, D.O. (1995) Purification and biochemical characterization of myomesin, a myosin and titin binding protein, from bovine skeletal muscle. Eur. J. Biochem., 233, 110-115.
17. Obermann, W.M.J., Gautel, M., Steiner, F., Van der Ven, P.F.M., Weber, K. and Fürst, D.O. (1996) The structure of the sarcomeric M band: localization of defined domains of myomesin, M-protein and the 250 kDa carboxyterminal region of titin by immunoelectron microscopy. J. Cell Biol., 134, 1441-1453.
18. Pearson, R.B. and Kemp, B.E. (1991) Protein kinase phosphorylation site sequences and consensus specifity motifs: tabulations. Methods Enzymol., 200, 62-81.
19. Saiki, R.K., Scharf, S.J., Faloona, F., Mullis, G.T. and Ehrlich, H.A. (1985) Enzymatic amplification of beta-globin genomic sequences and restriction site analysis for diagnosis of sickle cell anemia. Science, 230, 1350-1354.
20. Shaw, G., Debus, E. and Weber, K. (1984) The immunological relatedness of neurofilament proteins of higher vertebrates. Eur. J. Cell Biol., 34, 130-136.
21. Small, J.V., Fürst, D.O. and Thornell, L.-E. (1992) The cytoskeletal lattice of muscle cells. Eur. J. Biochem., 208, 559-572.
22. Studier, F.W., Rosenberg, A.H., Dunn, J.J. and Dubendorff, J.W (1990) Use of T7 RNA polymerase to direct expression of cloned genes. Methods Enzymol., 185, 60-89.
23. Trinick, J. and Lowey, S. (1977) M-protein from chicken pectoralis muscle: isolation and characterization. J. Mol. Biol., 113, 343-368.
24. Vinkemeier, U., Obermann, W., Weber, K. and Fürst, D.O. (1993) The globular head domain of titin extends into the center of the sarcomeric M band. J. Cell Sci., 106, 319-330.
25. Wehland, J., Schröder, H.C. and Weber, K. (1984) Amino sequence requirements in the epitope recognized by the the α-tubulin-specific rat monoclonal antibody YL 1/2. EMBO J., 3, 1295-1300.
26. Wessel, D. and Flügge, U.J. (1984) A method for the quantitative recovery of protein in dilute solution in the presence of detergents and lipids. Anal. Biochem., 138, 141-143.