Series of exon-skipping events in the elastic spring region of titin as the structural basis for myofibrillar elastic diversity

Circulation Research

Volumn 86, Issue 11, 2000, Pages 1114-1121

  Freiburg, Alexandra ^a   Trombitas, Karoly ^b   Hell, Wolfgang ^c   Cazorla, Olivier ^b   Fougerousse, Francoise ^d   Centner, Thomas ^a   Kolmerer, Bernhard ^a   Witt, Christian ^f   Beckmann, Jaques S ^d   Gregorio, Carol C ^e   Granzier, Henk ^b   Labeit, Siegfried ^a  

^a EUROPEAN MOLECULAR BIOLOGY LABORATORY   (Germany)

^b WASHINGTON STATE UNIVERSITY   (United States)

^c Medigenomix   (Germany)

^d GÉNÉTHON   (France)

^e UNIVERSITY OF ARIZONA   (United States)

^f EMBL ^*

Author keywords

Atrium; Diastole; Elasticity; Titin

Indexed keywords

CONNECTIN;

ANIMAL CELL; ARTICLE; CONTROLLED STUDY; ELASTICITY; EXON; HEART ATRIUM MUSCLE; HEART VENTRICLE WALL; MUSCLE FIBER CONTRACTION; MUSCLE TONE; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; PSOAS MUSCLE; RAT; SOLEUS MUSCLE; SWINE;

EID: 18544406997     PISSN: 00097330     EISSN: None     Source Type: Journal    
DOI: 10.1161/01.RES.86.11.1114     Document Type: Article

References (17)

1. Erickson HP. Stretching single protein molecules: titin is a weird spring. Science. 1997;276:1090-1092.
2. Linke WA, Granzier H. A spring tale: new facts on titin elasticity. Biophys J. 1998;75:2613-2614.
3. Granzier HL, Irving TC. Passive tension in cardiac muscle: contribution of collagen, titin, microtubules, and intermediate filaments. Biophys J. 1995;68:1027-1044.
4. Linke WA, Ivemeyer M, Olivieri N, Kolmerer B, Rüegg JC, Labeit S. Towards a molecular understanding of the elasticity of titin. J Mol Biol. 1996;261:62-71.
5. Trombitas K, Greaser M, Labeit S, Jin JP, Kellermayer M, Helmes M, Granzier H. Titin extensibility in situ: entropic elasticity of permanently folded and permanently unfolded molecular segments. J Cell Biol. 1998; 140:853-859.
6. Labeit S, Kolmerer B. Titins, giant proteins in charge of muscle ultrastructure and elasticity. Science. 1995;270:293-296.
7. Linke WA, Ivemeyer M, Mundel P, Stockmeier MR, Kolmerer B. Nature of PEVK-titin elasticity in skeletal muscle. Proc Natl Acad Sci U S A. 1995;95:8052-8057.
8. Linke WA, Rudy DE, Centner T, Gautel M, Witt CC, Labeit S, Gregorio CC. I-band titin in cardiac muscle is a three-element molecular spring and is critical for maintaining thin filament structure. J Cell Biol. 1999;246: 631-644.
9. Helmes M, Trombitas K, Centner T, Kellermayer M, Labeit S, Linke WA, Granzier H. Mechanically driven contour-length adjustment in rat cardiac titin's unique N2B sequence: titin is an adjustable spring. Circ Res. 1999;84:1339-1352.
10. Hu DH, Kimura S, Maruyama K. Sodium dodecyl sulfate gel electrophoresis studies of connectin-like high molecular weight proteins of various types of vertebrate and invertebrate muscles. J Biochem (Tokyo). 1986;99:1485-1492.
11. Wang K, McCarter R, Wright J, Beverly J, Ramirez-Mitchell R. Regulation of skeletal muscle stiffness and elasticity by titin isoforms: a test of the segmental extension model of resting tension. Proc Natl Acad Sci U S A. 1991;88:7101-7105.
12. Horowits R. Passive force generation and titin isoforms in mammalian skeletal muscle. Biophys J. 1992;61:392-398.
13. Cazorla O, Freiburg A, Helmes M, Centner T, McNabb M, Trombitas K, Labeit S, Granzier H. Differential expression of cardiac titin isoforms and modulation of cellular stiffness. Circ Res. 2000;86:59-67.
14. Rossi E, Faiella A, Zeviani M, Labeit S, Floridia G, Brunelli S, Cammarata M, Boncinelli E, Zuffardi O. Order of six loci at 2q24-q31 and orientation of the HOXD locus. Genomics. 1994;24:34-40.
15. Brady AJ. Length dependence of passive stiffness in single cardiac myocytes. Am J Physiol. 1991;260:H1062-H1071.
16. Fabiato A, Fabiato F. Myofilament-generated tension oscillations during partial calcium activation and activation dependence of the sarcomere length-tension relation of skinned cardiac cells. J Gen Phvsiol. 1978;72: 667-699.
17. Greaser ML, Wang S-M, Berri M, Mozdziak PE, Kumazawa Y. Sequence and mechanical implications of cardiac PEVK. In: Pollack GH, Granzier H, eds. Proceedings: Elastic Filaments of the Cell. Kluwer Academic/ Plenum Publishers. In press.