An N-terminal fragment of titin coupled to green fluorescent protein localizes to the Z-bands in living muscle cells: Overexpression leads to myofibril disassembly

Molecular Biology of the Cell

Volumn 8, Issue 4, 1997, Pages 705-717

  Turnacioglu, Kenan K ^a   Mittal, Balraj ^b   Dabiri, Guissou A ^a   Sanger, Jean M ^a   Sanger, Joseph W ^a  

^a UNIVERSITY OF PENNSYLVANIA   (United States)

^b SANJAY GANDHI POSTGRADUATE INSTITUTE OF MEDICAL SCIENCES   (India)

Author keywords

[No Author keywords available]

Indexed keywords

ALPHA ACTININ; COMPLEMENTARY DNA; CONNECTIN; GREEN FLUORESCENT PROTEIN; HYBRID PROTEIN; MUSCLE PROTEIN;

AMINO TERMINAL SEQUENCE; ANIMAL CELL; ANIMAL TISSUE; ARTICLE; CHICKEN; HEART MUSCLE CELL; MYOTUBE; NONHUMAN; PRIORITY JOURNAL; PROTEIN DEGRADATION; PROTEIN INDUCTION; PROTEIN LOCALIZATION; PROTEIN PROTEIN INTERACTION; RAT; SEQUENCE HOMOLOGY;

ANIMALIA; GALLUS GALLUS;

EID: 0031004299     PISSN: 10591524     EISSN: None     Source Type: Journal    
DOI: 10.1091/mbc.8.4.705     Document Type: Article

References (40)

1. Ayme-Southgate, A., Vigoreaux, J., Benian, G.M., and Pardue, M.L. (1991). Drosophila has a twitchin/titin-related gene that appears to encode projectin. Proc. Natl. Acad. Sci. USA 88, 7973-7977.
2. Benian, G.M., Kiff, J.E., Neckleman, N., Moerman, D.G., and Waterson, R.H. (1989). Sequence of an unusually large protein implicated in regulation of myosin activity in C. elegans. Nature 313, 545-551.
3. Chomczynski, P., and Sacchi, N. (1987). Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal. Biochem. 162, 156-159.
4. Colley, N.J., Tokuyasu, K.T., and Singer, S.J. (1990). The early expression of myofibrillar proteins in round postmitotic myoblasts of embyronic skeletal muscle. J. Cell Sci. 95, 11-22.
5. Danowski, B.A., Imanaka-Yoshida, K., Sanger, J.M., and Sanger, J.W. (1992). Costameres are sites of force transmission to the substratum in adult rat cardiomyocytes. J. Cell Biol. 118, 1411-1420.
6. Eilersten, K.J., Kazmierski, S.T., and Keller, C.S.T. (1994). Cellular titin localization in stress fibers and interaction with myosin II filaments in vitro. J. Cell Biol. 126, 1201-1210.
7. Einheber, S., and Fischman, D.A. (1990). Isolation and characterization of a cDNA clone encoding avian skeletal muscle C-protein: an intracellular member of the immunoglobulin superfamily. Proc. Natl. Acad. Sci. USA 87, 2157-2161.
8. Erickson, P.J. (1994). Reversible unfolding fibronectin type III and immunoglobulin domains provides the structural basis for stretch and elasticity of titin and fibronectin. Proc. Natl. Acad. Sci. USA 91, 10114-10118.
9. Fulton, A.B and Isaacs, W.B. (1991). Titin, a huge elastic sarcomeric protein with a probable role in morphogenesis. Bioassays 13, 157-161.
10. Furst, D.O., Osborn, M., and Weber, K. (1989). Myogenesis in the mouse embryo: differential onset of expression of myogenic proteins and the involvement of titin in myofibril assembly. J. Cell Biol. 109, 517-527.
11. Gilbert, A.U., Kelly, M.G., Mikawa, T., and Fischman, D.A. (1996). The carboxyl terminus of myosin-binding protein-C (MYBP-C, C-protein) specifies incorporation into the A-band of striated muscle J. Cell Sci. 109, 101-111.
12. Granzier, H.L., and Irving, T.C. (1995). Passive tension in cardiac muscle: contribution of collagen, titin, microtubules, and intermediate filaments. Biophys. J. 68, 1027-1044.
13. Harpaz, Y., and Chothia, C. (1994). Many of the immunoglobulin superfamily domains in cell adhesion molecules and surface receptors belong to a new structural set which is close to that containing variable domains. J. Mol. Biol. 238., 528-539.
14. Hill, C.S., Duran, S., Lin, Z., Weber, K., and Holtzer, H. (1986). Titin and myosin, but not desmin, are linked during myofibrillogenesis in postmitotic mononucleated myoblasts. J. Cell Biol. 103, 2185-2196.
15. Horowits, R., and Podolsky, R. (1987). The positional stability of thick filaments in activated skeletal muscel: evidence for the role of titin filaments. J. Cell Biol. 105, 2217-2223.
16. Huxley, H.E. (1972). The Structure and Function of Muscle, vol. 1, ed. G. H. Bourne, New York: Academic Press, 301-387.
17. Isaacs, W.B., Kim, I.S., Struve, A., and Fulton, A.B. (1989). Biosynthesis of titin in cultured skeletal muscle cells. J. Cell Biol. 109, 2189-2195.
18. Labeit, S., and Kolmerer, B. (1995a). Titins: giant proteins in charge of muscle ultrastructure and elasticity. Science 270, 293-296.
19. Labeit, S., and Kolmerer, B. (1995b). The complete primary structure of human nebulin and its correlation to muscle structure. J. Mol. Biol. 248, 308-315.
20. Lakey, A., Labeit, S., Gautel, M., Ferguson, C., Barlow, D., Leonard, K., and Bullard, B. (1993). Kettin, a large modular protein in the Z-disc of insect muscles. EMBO J. 12, 2863-2871.
21. LoRusso, S.M., Rhee, D., Sanger, J.M., and Sanger, J.W. (1997). Premyofibrils in spreading adult cardiomyocytes in tissue culture: evidence for reexpression of the embryonic program for myofibillogenesis in adult cells. Cell Motil. Cytoskeleton. (in press).
22. Maher, P., Cox., G.F., and Singer, S.J. (1985). Zeugmatin: A new high molecular weight protein associated with Z-lines in adult and early embryonic striated muscle. J. Cell Biol. 101, 1871-1883.
23. Maruyama, K. (1994). Connectin, an elastic protein of striated muscle. Biophys. Chem. 50, 73-85.
24. Maruyama, K., Endo, T., Kume, H., Kawamura, Y., Kanzawa, N., Kimura, S., Kawashima, S., and Maruyama, K. (1994). A partial connectin cDNA encoding a novel type of RSP motifs isolated from chicken embryonic skeletal muscle. J. Biochem. 115, 147-149.
25. Nave, R., Furst, D.O., and Weber, K. (1989). Visualization of the polarity of isolated titin molecules: a single globular head on a long thin rod as the M-band anchoring domain. J. Cell Biol. 109, 2177-2187.
26. Olson, N.J., Pearson, R.B., Needleman, D.S., Hurwitz, M.Y., Kemp, B.E., and Means, A.R. (1990). Regulatory and structural motifs of chicken gizzard myosin light-chain kinase. J. Biol. Chem. 263, 10510-10516.
27. Price, M., and Gomer, R.H. (1993). Skelemin, a cytoskeletal M-disc periphery protein, contains motifs of adhesion/recognition and intermediate filament proteins. J. Biol. Chem. 268, 21800-21810.
28. Rhee, D., Sanger, J.M., and Sanger, J.W. (1994). The premyofibril: evidence for its role in myofibrillogenesis. Cell Motil. Cytoskeleton 28, 1-24.
29. Sambrook, J., Fritsch, E.F., and Maniatis, T. (1986). Molecular Cloning: A Laboratory Manual. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory Press.
30. Sanger, J.M., Mittal, B., Pochapin, M.B., and Sanger, J.W. (1986). Myofibrillogenesis in living cells microinjected with fluorescently labeled alpha-actinin. J. Cell Biol. 102, 2053-2066.
31. Sanger, J.W., Mittal, B., and Sanger, J.M. (1984). Formation of myofibrils in spreading chick cardiac myocytes. Cell Motil. 4, 405-416.
32. Sanger, J.W., Sanger, J.M., and Jockusch, B.M. (1983). Differences in the stress fibers between fibroblasts and epithelial cells. J. Cell Biol. 96, 961-969.
33. Soteriou, A., Clarke, A., Martin, S., and Trinick, J. (1993). Titin folding energy and elasticity. Proc. R. Soc. Lond. B 254, 83-86.
34. Terai, M., Komiyama, M., and Shimada, Y. (1989). Myofibril assembly is linked with vinculin, α-actinin, and cell substrate contacts in embryonic cardiac myocytes in vitro. Cell Motil. Cytoskeleton 12, 185-194.
35. Tokuyasu, K.T., and Maher, P.A. (1987a). Immunocytochemical studies of cardiac myofibrillogenesis in early chick embryos. I. Presence of Immunofluorescent titin spots in premyofbril stages. J. Cell Biol. 105, 2781-2793.
36. Tokuyasu, K.T., and Maher, P.A. (1987b). Immunocytochemical studies of cardiac myofibrillogenesis in early chick embryos. II. Generation of α-actinin dots within titin spots at the time of the first myofibril formation. J. Cell Biol. 105, 2795-2801.
37. Trinick, J. (1994). Titin and nebulin: protein rulers in muscle? Trends Cell Biol. 19, 405-408.
38. Turnacioglu, K.K., Mittal, B., Sanger, J.M., and Sanger, J.W. (1996). Partial characterization of zeugmatin indicates that it is part of the Z-band region of titin. Cell Motil. Cytoskeleton 34, 108-121.
39. Turnacioglu, K.K., Mittal, B., Dabiri, G., Sanger, J.M, and Sanger J.W. (1997). Zeugmatin is part of the Z-band targeting region of titin. Cell Struct. Funct. 22 (in press).
40. Wang, K., McClure, J., and Tu, A. (1979). Titin: major myofibrillar components of striated muscle. Proc. Natl. Acad. Sci. USA 76, 3698-3702.