Identification of ribonucleotide reductase protein R1 as an activator of microtubule nucleation in Xenopus egg mitotic extracts

Molecular Biology of the Cell

Volumn 11, Issue 12, 2000, Pages 4173-4187

  Takada, S ^a   Shibata, T ^a   Hiraoka, Y ^a   Masuda, H ^a  

^a INST OF PHYS AND CHEMICAL RESEARCH   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

ALPHA TUBULIN; BETA TUBULIN; GAMMA TUBULIN; RIBONUCLEOTIDE REDUCTASE;

ANIMAL CELL; ARTICLE; CELL CYCLE; CENTROSOME; ENZYME SUBUNIT; INTERPHASE; MICROTUBULE; MITOSIS; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN PROTEIN INTERACTION; XENOPUS;

XENOPUS;

EID: 0033638659     PISSN: 10591524     EISSN: None     Source Type: Journal    
DOI: 10.1091/mbc.11.12.4173     Document Type: Article

References (83)

1. 2/M and enters vesicles at anaphase
2. Cytoplasmic accumulation of cyclin B1 in human cells: Association with a detergent-resistant compartment and with the centrosome
3. Structural and chemical characterization of isolated centrosomes
4. Supernumerary centrosomes and cancer: Boveri's hypothesis resurrected
5. Both viral (adenovirus E1B) and cellular (hsp 70, p53) components interact with centrosomes
6. Role of effector binding in allosteric control of ribonucleotide diphosphate reductase
7. Regulation of the microtubuie nucleating activity of centrosomes in Xenopus egg extracts: Role of cyclin A-associated protein kinase
8. Cloned mouse ribonucleotide reductase subunit M1 cDNA reveals amino acid sequence homology with Escherichia coli and herpesvirus ribonucleotide reductases
9. Yeast Sml1, a protein inhibitor of ribonucleotide reductase
10. Purification, characterization, and localization of subunit interaction area of recombinant mouse ribonucleotide reductase R1 subunit
11. Pericentrin, and γ-tubulin. form a protein complex and are organized into a novel lattice at the centrosome
12. The spindle pole body of Schizosaccharomyces pombe enters and leaves the nuclear envelope as the cell cycle proceeds
13. Pericentrin, a highly conserved centrosome protein involved in microtubule organization
14. DNA damage and cell cycle regulation of ribonucleotide reductase
15. Localization of ribonucleotide reductase in mammalian cells
16. Cell cycle-dependent expression of mammalian ribonucleotide reductase. Differential regulation of the two subunits
17. Protofilaments and rings, two conformations of the tubulin family conserved from bacterial FtsZ to α/β and γ-tubulin
18. Cell-cycle dependent regulation of mammalian ribonucleotide reductase. The S phase-correlated increase in subunit M2 is regulated by de novo protein synthesis
19. Binding of allosteric effectors to ribonucleotide reductase protein R1: Reduction of active-site cysteines promotes substrate binding
20. Human 76p: A new member of the γ-tubulin-associated protein family
21. Centrosome assembly in vitro: Role of γ-tubulin recruitment in Xenopus sperm aster formation
22. + of the fission yeast Schizosaccharomyces pombe encode the large and small subunits of ribonucleotide reductase
23. Cyclin B2 undergoes cell cycle-dependent nuclear translocation and, when expressed as a nondestructible mutant, causes mitotic arrest in HeLa cells
24. The spindle pole body component Spc98p interacts with the γ-tubulin-like Tub4p of Saccharomyces cerevisiae at the sites of microtubule attachment
25. The use of cell division cycle mutants to investigate the control of microtubule distribution in the fission yeast Schizosaccharomyces pombe
26. Poles apart? Spindle pole bodies and centrosomes differ in ultrastructure yet their function and regulation are conserved
27. The fission yeast γ-tubulin is essential for mitosis and is localized at microtubule organizing centers
28. Human γ-tubulin functions in fission yeast
29. Identification of RNR4, encoding a second essential small subunit of ribonucleotide reductase in Saccharomyces cerevisiae
30. The DNA replication and damage checkpoint pathways induce transcription by inhibition of the Crt1 repressor
31. Preparation of modified tubulins
32. A kinetic study on the influence of nucleoside triphosphate effectors on subunit interaction in mouse ribonucleotide reductase
33. γ-Tubulin is a centrosomal protein required for cell cycle-dependent microtubule nucleation
34. Molecular components of the centrosome
35. The centrosome and cellular organization
36. The sudden recruitment of γ-tubulin to the centrosome at the onset of mitosis and its dynamic exchange throughout the cell cycle, do not require microtubules
37. The spindle pole body component Spc97p interacts with the gamma-tubulin of Saccharomyces cerevisiae and functions in microtubule organization and spindle pole body duplication
38. Microtubule-nucleating activity of centrosomes in Chinese hamster ovary cells is independent of the centriole cycle but coupled to the mitotic cycle
39. Cyclins A and B associate with chromatin and the polar regions of spindles, respectively, and do not undergo complete degradation at anaphase in syncytial Drosophila embryos
40. Analysis of Tub4p, a yeast γ-tubulin-like protein: Implications for microtubule-organizing center function
41. Xgrip109: A γ tubulin-associated protein with an essential role in γ tubulin ring complex (γTuRC) assembly and centrosome function
42. In vitro reactivation of spindle elongation in fission yeast nuc2 mutant cells
43. In vitro microtubulenucleating activity of spindle pole bodies in fission yeast Schizosaccharomyces pombe: Cell cycle-dependent activation in Xenopus cell-free extracts
44. Role of γ-tubulin in mitosis-specific microtubule nucleation from the Schizosaccharomyces pombe spindle pole body
45. Microtubule nucleation by γ-tubulin-containing rings in the centrosome
46. Recruitment of the γ-tubulin ring complex to Drosophila salt-stripped centrosome scaffolds
47. γ-Tubulin in mammalian cells: The centrosomal and the cytosolic forms
48. Liz1p, a novel fission yeast membrane protein, is required for normal cell division when ribonucleotide reductase is inhibited
49. The mammalian γ-tubulin complex contains homologues of the yeast spindle pole body components Spc97p and Spc98p
50. Cell cycle extracts
51. When overexpressed, a novel centrosomal protein, RanBPM, causes ectopic microtubule nucleation similar to γ-tubulin
52. Cell division cycle mutants altered in DNA replication and mitosis in the fission yeast Schizosaccharomyces pombe
53. γ-Tubulin. The microtubule organizer?
54. γ-Tubulin is a component of the spindle pole body that is essential for microtubule function on Aspergillus nidulans
55. Microtubule nucleating activity of centrosomes in cell-free extracts from Xenopus eggs: Involvement of phosphorylation and accumulation of pericentriolar material
56. Human M1 subunit of ribonucleotide reductase: cDNA sequence and expression in stimulated lymphocytes
57. Nuclear transport protein does double duty in mitosis
58. Centrosome-microtubule nucleation
59. Spc98p directs the yeast γ-tubulin complex into the nucleus and is subject to cell-cycle-dependent phosphorylation on the nuclear side of the spindle pole body
60. Interactions between deoxyribonucleotide and DNA synthesis
61. From RNA to DNA, why so many ribonucleotide reductases?
62. Mechanisms of nuclear positioning
63. DNA-replication/DNA-damage-dependent centrosome inactivation in Drosophila embryos
64. Initiation and growth of microtubules from mitotic centers in lysed mammalian cells
65. A high divergent γ-tubulin gene is essential for cell growth and proper microtubule organization in Saccharomyces cerevisiae
66. γ-Tubulin-like Tub4p of Saccharomyces cerevisiae is associated with the spindle pole body substructures that organize microtubules and is required for mitotic spindle formation
67. In vitro reconstitution of centrosome assembly and function: The central role of γ-tubulin
68. γ-Tubulin is required for the structure and function of the microtubule organizing center in Drosophila neuroblasts
69. A ribonucleotide reductase gene involved in a p53-dependent cell-cycle checkpoint for DNA damage
70. Characterization of the human homologue of the yeast Spc98p and its association with γ-tubulin
71. Reduction of ribonucleotides
72. Ribonucleotide reductase from calf thymus
73. Subunit M2 of mammalian ribonucleotide reductase
74. CDC27Hs colocalizes with CDC16Hs to the centrosome and mitotic spindle and is essential for the metaphase to anaphase transition
75. Structure of ribonucleotide reductase protein R1
76. The fission yeast γ-tubulin complex is required in G1 phase and is a component of the spindle-assembly checkpoint
77. Motors involved in spindle assembly and chromosome segregation
78. Centrosomes isolated from Spisula solidissima oocytes contain rings and an unusual stoichiometric ratio of α/β tubulin
79. Rnr4p, a novel ribonucleotide reductase small-subunit protein
80. Pathways of spindle assembly
81. Dynamic association of proteasomal machinery with the centrosome
82. A suppressor of two essential checkpoint genes identifies a novel protein that negatively affects dNTP pools
83. Nucleation of microtubule assembly by γ-tubulin-containing ring complex