UCN-01 (7-hydroxystaurosporine) enhances 5-fluorouracil cytotoxicity through down-regulation of thymidylate synthetase messenger RNA

Japanese Journal of Cancer Research

Volumn 91, Issue 11, 2000, Pages 1192-1198

  Abe, S ^a   Kubota, T ^a   Otani, Y ^a   Furukawa, T ^a   Watanabe, M ^a   Kumai, K ^a   Kitajima, M ^a  

^a KEIO UNIVERSITY   (Japan)

Author keywords

5 Fluorouracil; mRNA; Pancreatic cancer; Thymidylate synthetase; UCN 01

Indexed keywords

3 (4,5 DIMETHYL 2 THIAZOLYL) 2,5 DIPHENYLTETRAZOLIUM BROMIDE; 7 HYDROXYSTAUROSPORINE; ANTINEOPLASTIC AGENT; CALCIUM CHANNEL BLOCKING AGENT; CALCIUM ION; CISPLATIN; CYCLIN DEPENDENT KINASE; CYCLIN DEPENDENT KINASE 2; CYCLIN E; DIHYDROFOLATE REDUCTASE; DIMETHYL SULFOXIDE; DNA DIRECTED DNA POLYMERASE ALPHA; ENZYME INHIBITOR; FLOXURIDINE PHOSPHATE; FLUOROURACIL; FORMALDEHYDE; FORMAZAN; MESSENGER RNA; MITOMYCIN C; NEW DRUG; PHOSPHOLIPID DERIVATIVE; PROTEIN KINASE C; PROTEIN KINASE C INHIBITOR; PROTEIN P21; RIBOSE PHOSPHATE; RNA; SODIUM CHLORIDE; THYMIDYLATE SYNTHASE; TRANSCRIPTION FACTOR E2F; UNINDEXED DRUG;

ANTINEOPLASTIC ACTIVITY; ARTICLE; CARCINOMA CELL; CELL CYCLE; CELL GROWTH; CELL PROLIFERATION; CONCENTRATION (PARAMETERS); CONTROLLED STUDY; CYTOTOXICITY; DOWN REGULATION; DRUG ACTIVITY; ENZYME ACTIVITY; ENZYME INHIBITION; GROWTH INHIBITION; HUMAN; HUMAN CELL; PANCREAS CARCINOMA; PHOSPHORYLATION; PRIORITY JOURNAL;

EID: 0033635741     PISSN: 09105050     EISSN: None     Source Type: Journal    
DOI: 10.1111/j.1349-7006.2000.tb00904.x     Document Type: Article

References (38)

1. MPF from starfish oocytes at first meiotic metaphase is a heterodimer containing one molecule of cdc 2 and one molecule of cyclin B
2. Cyclin is a component of maturation-promoting factor in Xenopus
3. Cyclins and cyclin dependent kinase: A biochemical view
4. Cancer cell cycles
5. Cyclin dependent kinases: Engines, clocks and microprocessors
6. Cyclins, cyclin-dependent kinases and CDK inhibitors: Implications in cell cycle control and cancer
7. Genetic alterations of cyclins, cyclin dependent kinases, and cdk inhibitors in human cancer
8. A novel cyclin associates with MO15/CDK7 to form the CDK-activating kinase
9. UCN-01, 7-hydroxylstaurosporine, inhibits kinase activity of cycline-dependent kinase and reduces the phosphorylation of the retinoblastoma susceptibility gene product in an A549 human lung carcinoma cell line
10. DRTF1/E2F: An expanding family of heterodimeric transcription factors implicated in cell-cycle control
11. Analogs of staurosporine: Potential anticancer drugs?
12. Modulation of apoptosis in rat thymocytes by analogs of staurosporine: Lack of direct association with inhibition of protein kinase C
13. The effect of UCN-01 (7-hydroxystaurosporine), a potent inhibitor of protein kinase C, on fractionated radiotherapy or daily chemotherapy of a murine fibroblast
14. G1 phase accumulation induced by UCN-01 is associated with dephosphorylation of Rb and CDK2 proteins as well as induction of CDK inhibitor p21/Cip1/WAF1/Sdi1 in p53-mutated human epidermoid carcinoma A431 cells
15. Phase 1 trial of infusional UCN-01, a novel protein kinase inhibitor in patients with refractory neoplasmas
16. Diverse effects of indolocarbazole compounds on the cell cycle progression of ras-transformed rat fibroblast cells
17. Effect of UCN-01, a selective inhibitor of protein kinase C, on the cell-cycle distribution of human epidermoid carcinoma A431 cells
18. Enhancement of antitumor activity of mitomycin C in vitro and in vivo by UCN-01, a selective inhibitor of protein kinase C
19. Enhancement of cisplatin-induced cytotoxicity by 7-hydroxystaurosporine (UCN-01), a new G2-checkpoint inhibitor
20. UCN-01 in ovary cancer cells: Effective as a signal agent and in combination with cis-diamminedichloroplatinum; independent of p53 status
21. Thymidylate synthase gene and protein expression correlate and are associated with response to 5-fluorouracil in human colorectal and gastric tumors
22. Human pancreatic carcinoma (MIA PaCa-2) in continuous culture: Sensitivity to asparaginase
23. Rapid colorimetric assay for cellular growth and survival: Application to proliferation and cytotoxicity assays
24. Predictability of in vivo chemosensitivity by in vitro MTT assay with reference to the clonogenic assay
25. MTT assay with reference to the clinical effect of chemotherapy
26. Clinical usefulness of chemosensitivity testing using the MTT assay
27. Relationship between protein levels and gene expression of dihydropyrimidine dehydrogenase in human tumor cells during growth in culture and in nude mice
28. Nucleotide sequence of a functional cDNA for human thymidylate synthase
29. Enhanced expression of a glyceraldehyde-3-phosphate dehydrogenase gene in human lung cancers
30. In vivo kinetics of thymidylate synthetase inhibition in 5-fluorouracil-sensitive and -resistant murine colon adenocarcinomas
31. Significance of measuring 5-fluorouracil incorporated into RNA of tumor tissue as a parameter for the antitumor activity of 5-fluorouracil and its derivatives
32. Preliminary evidence that incorporation of 5-fluorouracil into RNA correlates with antitumor response
33. The retinoblastoma protein and cell cycle control
34. The product of the retinoblastoma susceptibility gene has properties of a cell cycle regulatory element
35. The retinoblastoma protein is phosphorylated during specific phase of the cell cycle
36. UCN-01 suppresses thymidylate synthase gene expression and enhances 5-fluorouracil-induced apoptosis in a sequence-dependent manner
37. Thymidylate synthethase: Mechanism of inhibition by 5-fluoro-2'-deoxyuridylate
38. Clinical pharmacology of UCN-01: Initial observations and comparison to preclinical models