UCN-01 suppresses thymidylate synthase gene expression and enhances 5- fluorouracil-induced apoptosis in a sequence-dependent manner

Clinical Cancer Research

Volumn 4, Issue 9, 1998, Pages 2201-2206

  Hsueh, Chung Tsen ^a   Kelsen, David ^a   Schwartz, Gary K ^a  

^a Division of Solid Tumor Oncology   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

7 HYDROXYSTAUROSPORINE; FLUOROURACIL; THYMIDYLATE SYNTHASE;

APOPTOSIS; ARTICLE; CANCER CELL CULTURE; CANCER CHEMOTHERAPY; DOSE RESPONSE; ENZYME ACTIVITY; ENZYME INHIBITION; GENE EXPRESSION REGULATION; HUMAN; HUMAN CELL; NORTHERN BLOTTING; PRIORITY JOURNAL; STOMACH CANCER;

ALKALOIDS; ANTIMETABOLITES, ANTINEOPLASTIC; ANTINEOPLASTIC COMBINED CHEMOTHERAPY PROTOCOLS; APOPTOSIS; CARRIER PROTEINS; CELL CYCLE PROTEINS; CYCLIN-DEPENDENT KINASES; DNA-BINDING PROTEINS; DOWN-REGULATION; DRUG ADMINISTRATION SCHEDULE; DRUG SYNERGISM; E2F TRANSCRIPTION FACTORS; E2F1 TRANSCRIPTION FACTOR; ENZYME INHIBITORS; FLUOROURACIL; GENE EXPRESSION; HUMANS; PROTEIN KINASE C; STAUROSPORINE; STOMACH NEOPLASMS; THYMIDYLATE SYNTHASE; TRANSCRIPTION FACTOR DP1; TRANSCRIPTION FACTORS; TUMOR CELLS, CULTURED;

EID: 0031670310     PISSN: 10780432     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (31)

1. Mini, E., Trave, F., Rustum, Y. M., and Bertino, J. R. Enhancement of the antitumor effects of 5-fluorouracil by folinic acid. Pharmacol. Ther., 47: 1-19, 1990.
2. Johnston, P. G., Lenz, H. J., Leichman, C. G., Danenberg, K. D., Allegra, C. J., Danenberg, P. V., and Leichman, L. Thymidylate synthase gene and protein expression correlate and are associated with response to 5-fluorouracil in human colorectal and gastric tumors. Cancer Res., 55: 1407-1412, 1995.
3. Leichman, C. G., Lenz, H. J., Leichman, L., Danenberg, K., Baranda, J., Groshen, S., Boswell, W., Metzger, R., Tan, M., and Danenberg, P. V. Quantitation of intratumoral thymidylate synthase expression predicts for disseminated colorectal cancer response and resistance to protracted-infusion fluorouracil and weekly leucovorin. J. Clin. Oncol., 15: 3223-3229, 1997.
4. Schwartz, G. K. Protein kinase C inhibitors as inducers of apoptosis for cancer therapy. Exp. Opin. Invest. Drugs, 5: 1601-1615, 1996.
5. Akinaga, S., Nomura, K., Gomi, K., and Okabe, M. Synergistic antitumor effect of UCN-01, a protein kinase C inhibitor, combined with various anticancer agents. Proc. Am. Assoc. Cancer Res., 33: 514, 1992.
6. Bible, K. C., and Kaufmann, S. H. Cytotoxic synergy between flavopiridol (NSC 649890, L86-8275) and various antineoplastic agents: the importance of sequence of administration. Cancer Res., 57: 3375-3380, 1997.
7. Altorki, N., Schwartz, G. K., Blundell, M., Davis, B. M., Kelsen, D. P., and Albino, A. P. Characterization of cell lines established from human gastric-esophageal adenocarcinomas. Biologic phenotype and invasion potential. Cancer (Phila.), 72: 649-657, 1993.
8. Nabeya, Y., Loganzo, F., Jr., Maslak, P., Lai, L., de Oliveira, A. R., Schwartz, G. K., Blundell, M. L., Altorki, N. K., Kelsen, D. P., and Albino, A. P. The mutational status of p53 protein in gastric and esophageal adenocarcinoma cell lines predicts sensitivity to chemotherapeutic agents. Int. J. Cancer, 64: 37-46, 1995.
9. 3H]thymidine incorporation assay. J. Immunol. Methods, 170: 211-224, 1994.
10. Laemmli, U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature (Lond.), 227: 680-685, 1970.
11. Sambrook, J., Fritsch, E. F., and Maniatis, T. Molecular Cloning: A Laboratory Manual. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory, 1989.
12. Harlow, E., and Lane, D. Antibodies: A Laboratory Manual. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory, 1988.
13. Mashima, T., Naito, M., Fujita, N., Noguchi, K., and Tsuruo, T. Identification of actin as a substrate of ICE and an ICE-like protease and involvement of an ICE-like protease but not ICE in VP-16-induced U937 apoptosis. Biochem. Biophys. Res. Commun., 217: 1185-1192, 1995.
14. Hsueh, C. T., and Dolnick, B. J. Regulation of folate-binding protein gene expression by DNA methylation in methotrexate-resistant KB cells. Biochem. Pharmacol., 47: 1019-1027, 1994.
15. Rosen, K. M., and Villa-Komaroff, L. An alternative method for visualization of RNA in formaldehyde agarose gels. Focus, 12: 23-24, 1990.
16. Dolnick, B. J., Zhang, Z-G., Hines, J. D., and Rustum, Y. M. Quantitation of dihydrofolate reductase and thymidylate synthase mRNAs in vivo and in vitro by polymerase chain reaction. Oncol. Res., 4: 65-72, 1992.
17. Guy, C. T., Zhou, W., Kaufman, S., and Robinson, M. O. E2F-1 blocks terminal differentiation and causes proliferation in transgenic megakaryocytes. Mol. Cell. Biol., 16: 685-693, 1996.
18. Haimovitz-Friedman, A., Kan, C. C., Ehleiter, D., Persaud, R. S., McLoughlin, M., Fuks, Z., and Kolesnick, R. N. Ionizing radiation acts on cellular membranes to generate ceramide and initiate apoptosis. J. Exp. Med., 180: 525-535, 1994.
19. Johnston, P. G., Liang, C-M., Henry, S., Chabner, B. A., and Allegra, C. J. Production and characterization of monoclonal antibodies that localize human thymidylate synthase in the cytoplasm of human cells and tissue. Cancer Res., 51: 6668-6676, 1991.
20. Washtien, W. L. Increased levels of thymidylate synthetase in cells exposed to 5-fluorouracil. Mol. Pharmacol., 25: 171-177, 1984.
21. Chu, E., Koeller, D. M., Johnston, P. G., Zinn, S., and Allegra, C. J. Regulation of thymidylate synthase in human colon cancer cells treated with 5-fluorouracil and interferon-γ. Mol. Pharmacol., 43: 527-533, 1993.
22. 1 phase accumulation induced by UCN-01 is associated with dephosphorylation of Rb and CDK2 proteins as well as induction of CDK inhibitor p21/Cip1/WAF1/Sdi1 in p53-mutated human epidermoid carcinoma A431 cells. Cancer Res., 57: 1495-1501, 1997.
23. Fan, G., and Steer, C. J. The retinoblastoma gene product is a negative modulator of the apoptotic pathway. Adv. Enzyme Regul., 36: 283-303, 1996.
24. 1-S-regulatory genes. Mol. Cell. Biol., 15: 4215-4224, 1995.
25. Li, W., Fan, J., Hochhauser, D., Banerjee, D., Zielinski, Z., Almasan, A., Yin, Y., Kelly, R., Wahl, G. M., and Bertino, J. R. Lack of functional retinoblastoma protein mediates increased resistance to antimetabolites in human sarcoma cell lines. Proc. Natl. Acad. Sci. USA, 92: 10436-10440, 1995.
26. Iwase, S., Furukawa, Y., Kikuchi, J., Nagai, M., Terui, Y., Nakamura, M., and Yamada, H. Modulation of E2F activity is linked to interferon-induced growth suppression of hematopoietic cells. J. Biol. Chem., 272: 12406-12414, 1997.
27. Chu, E., and Allegra, C. J. The role of thymidylate synthase in cellular regulation. Adv. Enzyme Regul., 36: 143-163, 1996.
28. Johnson, D. G., Ohtani, K., and Nevins, J. R. Autoregulatory control of E2F1 expression in response to positive and negative regulators of cell cycle progression. Genes Dev., 8: 1514-1525, 1994.
29. Dynlacht, B. D., Flores, O., Lees, J. A., and Harlow, E. Differential regulation of E2F transactivation by cyclin/cdk2 complexes. Genes Dev., 8: 1772-1786, 1994.
30. Hateboer, G., Kerkhoven, R. M., Shvarts, A., Bernards, R., and Beijersbergen, R. L. Degradation of E2F by the ubiquitin-proteasome pathway: regulation by retinoblastoma family proteins and adenovirus transforming proteins. Genes Dev., 10: 2960-2970, 1996.
31. Campanero, M. R., and Flemington, E. K. Regulation of E2F through ubiquitin-proteasome-dependent degradation: stabilization by the pRb tumor suppressor protein. Proc. Natl. Acad. Sci. USA, 94: 2221-2226, 1997.