Distinct pathogenic sequela in rhesus macaques infected with CCR5 or CXCR4 utilizing SHIVs

Science

Volumn 284, Issue 5415, 1999, Pages 816-819

  Harouse, Janet M ^a   Gettie, Agegnehu ^a   Tan, Rei Chin How ^a   Blanchard, James ^b   Cheng Mayer, Cecilia ^a  

^a ROCKEFELLER UNIVERSITY   (United States)

^b TULANE UNIVERSITY MEDICAL CENTER   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CHEMOKINE RECEPTOR CCR5; CHEMOKINE RECEPTOR CXCR4; HUMAN IMMUNODEFICIENCY VIRUS VACCINE; VIRUS ENVELOPE PROTEIN;

ANIMAL CELL; ANIMAL EXPERIMENT; ANIMAL MODEL; ANIMAL TISSUE; ARTICLE; HUMAN IMMUNODEFICIENCY VIRUS 1; HUMAN IMMUNODEFICIENCY VIRUS INFECTION; NONHUMAN; PRIORITY JOURNAL; RHESUS MONKEY; SIMIAN IMMUNODEFICIENCY VIRUS; VIREMIA; VIRUS ENVELOPE; VIRUS PATHOGENESIS; VIRUS REPLICATION;

ACQUIRED IMMUNODEFICIENCY SYNDROME; ANIMALS; CD4 LYMPHOCYTE COUNT; CD4-CD8 RATIO; CHIMERA; COLON; HIV-1; IMMUNITY, MUCOSAL; INTESTINAL MUCOSA; JEJUNUM; MACACA MULATTA; REASSORTANT VIRUSES; RECEPTORS, CCR5; RECEPTORS, CXCR4; SIMIAN ACQUIRED IMMUNODEFICIENCY SYNDROME; SIMIAN IMMUNODEFICIENCY VIRUS; VIRAL LOAD; VIREMIA; VIRUS REPLICATION;

EID: 0033617547     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.284.5415.816     Document Type: Article

References (45)

1. R. C. Desrosiers, Annu. Rev. Microbiol. 42, 607 (1988); J. Overbaugh, P. A. Luciw, E. A. Hoover AIDS 11 (suppl. A), S47 (1997).
2. R. C. Desrosiers, Annu. Rev. Microbiol. 42, 607 (1988); J. Overbaugh, P. A. Luciw, E. A. Hoover AIDS 11 (suppl. A), S47 (1997).
3. H. J. Alter et al., Science 226, 549 (1984); P. Fultz et al., J. Virol. 58, 116 (1986); M. B. Agy et al., ibid. 257, 103 (1992)
4. H. J. Alter et al., Science 226, 549 (1984); P. Fultz et al., J. Virol. 58, 116 (1986); M. B. Agy et al., ibid. 257, 103 (1992)
5. H. J. Alter et al., Science 226, 549 (1984); P. Fultz et al., J. Virol. 58, 116 (1986); M. B. Agy et al., ibid. 257, 103 (1992)
6. P. Putkonen et al., J. AIDS 2, 366 (1989); S. W. Barnett, K. K. Murthy, B. G. Herndier, J. A. Levy, Science 266, 642 (1994).
7. P. Putkonen et al., J. AIDS 2, 366 (1989); S. W. Barnett, K. K. Murthy, B. G. Herndier, J. A. Levy, Science 266, 642 (1994).
8. J. Li, C. I. Lord, W. Haseltine, N. L. Letvin, J. Sodroski, J. AIDS 5, 639 (1992); S. Sakuragi et al., J. Gen. Virol. 73, 2983 (1992); P. A. Luciw, E. Pratt-Lowe, K. E. S. Shaw, J. A. Levy, C. Cheng-Mayer, Proc. Natl. Acad. Sci. U.S.A. 92, 7490 (1995); K. A. Reimann et al., J. Virol. 70, 3198 (1996).
9. J. Li, C. I. Lord, W. Haseltine, N. L. Letvin, J. Sodroski, J. AIDS 5, 639 (1992); S. Sakuragi et al., J. Gen. Virol. 73, 2983 (1992); P. A. Luciw, E. Pratt-Lowe, K. E. S. Shaw, J. A. Levy, C. Cheng-Mayer, Proc. Natl. Acad. Sci. U.S.A. 92, 7490 (1995); K. A. Reimann et al., J. Virol. 70, 3198 (1996).
10. J. Li, C. I. Lord, W. Haseltine, N. L. Letvin, J. Sodroski, J. AIDS 5, 639 (1992); S. Sakuragi et al., J. Gen. Virol. 73, 2983 (1992); P. A. Luciw, E. Pratt-Lowe, K. E. S. Shaw, J. A. Levy, C. Cheng-Mayer, Proc. Natl. Acad. Sci. U.S.A. 92, 7490 (1995); K. A. Reimann et al., J. Virol. 70, 3198 (1996).
11. J. Li, C. I. Lord, W. Haseltine, N. L. Letvin, J. Sodroski, J. AIDS 5, 639 (1992); S. Sakuragi et al., J. Gen. Virol. 73, 2983 (1992); P. A. Luciw, E. Pratt-Lowe, K. E. S. Shaw, J. A. Levy, C. Cheng-Mayer, Proc. Natl. Acad. Sci. U.S.A. 92, 7490 (1995); K. A. Reimann et al., J. Virol. 70, 3198 (1996).
12. N. L. Letvin, Science 280, 1875 (1998).
13. R. Shibata et al., J. Infect. Dis. 176, 362 (1997); K. A. Reimann et al., J. Virol. 70, 6922 (1996); S. V. Joag et al., ibid., p. 3189; J. M. Harouse et al., Virology 248, 95 (1998).
14. R. Shibata et al., J. Infect. Dis. 176, 362 (1997); K. A. Reimann et al., J. Virol. 70, 6922 (1996); S. V. Joag et al., ibid., p. 3189; J. M. Harouse et al., Virology 248, 95 (1998).
15. R. Shibata et al., J. Infect. Dis. 176, 362 (1997); K. A. Reimann et al., J. Virol. 70, 6922 (1996); S. V. Joag et al., ibid., p. 3189; J. M. Harouse et al., Virology 248, 95 (1998).
16. R. Shibata et al., J. Infect. Dis. 176, 362 (1997); K. A. Reimann et al., J. Virol. 70, 6922 (1996); S. V. Joag et al., ibid., p. 3189; J. M. Harouse et al., Virology 248, 95 (1998).
17. M. T. Niu, D. S. Stein, S. M. Schnittman, J. Infect. Dis. 168, 1490 (1993); M. A. Nowak et al., J. Virol. 71, 7518 (1997); T. Schneider et al., Gut 37, 524 (1995).
18. M. T. Niu, D. S. Stein, S. M. Schnittman, J. Infect. Dis. 168, 1490 (1993); M. A. Nowak et al., J. Virol. 71, 7518 (1997); T. Schneider et al., Gut 37, 524 (1995).
19. M. T. Niu, D. S. Stein, S. M. Schnittman, J. Infect. Dis. 168, 1490 (1993); M. A. Nowak et al., J. Virol. 71, 7518 (1997); T. Schneider et al., Gut 37, 524 (1995).
20. Y. Feng, C. C. Broder, P. E. Kennedy, E. A. Berger, Science 272, 872 (1996).
21. N. L. Michael et al., J. Virol. 72, 6040 (1998); I. Theodoru, L. Meyer, M. Magierowska, C. Katlama, C. Rouzioux, Lancet 349, 1219 (1997); R. Biti et al., Nature Med. 3, 252 (1997); R. Liu et al., Cell 86, 367 (1996).
22. N. L. Michael et al., J. Virol. 72, 6040 (1998); I. Theodoru, L. Meyer, M. Magierowska, C. Katlama, C. Rouzioux, Lancet 349, 1219 (1997); R. Biti et al., Nature Med. 3, 252 (1997); R. Liu et al., Cell 86, 367 (1996).
23. N. L. Michael et al., J. Virol. 72, 6040 (1998); I. Theodoru, L. Meyer, M. Magierowska, C. Katlama, C. Rouzioux, Lancet 349, 1219 (1997); R. Biti et al., Nature Med. 3, 252 (1997); R. Liu et al., Cell 86, 367 (1996).
24. N. L. Michael et al., J. Virol. 72, 6040 (1998); I. Theodoru, L. Meyer, M. Magierowska, C. Katlama, C. Rouzioux, Lancet 349, 1219 (1997); R. Biti et al., Nature Med. 3, 252 (1997); R. Liu et al., Cell 86, 367 (1996).
25. SF162 propagated in human PBMCs [A. Luciw et al., Proc. Natl. Acad. Sci. U.S.A. 92, 7490 (1995)]. Two weeks after inoculation 10 ml of peripheral blood and 5 ml of bone marrow aspirate were collected from each animal, combined, and used to transfuse two naive recipients (P2). This inoculation schedule was performed two more times in naïve recipients group P3 and P4. All clinical procedures were performed in accordance with Institutional Animal Care and Use Committee (IACUC) guidelines at the Tulane Regional Primate Research Center.
26. S. V. Joag et al., J. Virol. 70, 3189 (1996); K. A. Reimann et al., ibid., p. 6922.
27. S. V. Joag et al., J. Virol. 70, 3189 (1996); K. A. Reimann et al., ibid., p. 6922.
28. Plasma was clarified from EDTA-treated whole blood by centrifugation. We then subjected clarified samples to p27 antigen enzyme-linked immunosorbent assay (ELISA) (Retro-TEK SIV p27 antigen ELISA; Cellular Products, Buffalo, NY), branched DNA (bDNA) analysis (Chiron, Emeryville, CA), and strip immunoblot assay to detect humoral immune responses (Chiron RIBA HIV-1/HIV-2 SIA, Chiron). To analyze T cell subsets we used the following human monoclonal antibodies (mAb): Leu-5b-FITC (fluorescein isothiocyanate), Leu-3a-PE (phycoerythrin), and Leu-2a-PerCP (peridinin chlorophyll protein), which recognize the CD2, CD4, and CD8 molecules, respectively (Becton Dickinson, San Jose, CA); 7E14-APC (allophycocyanin), which recognizes the CD4 protein (Exalpha, Boston, MA); and 12G5-PE, which recognizes the CXCR4 protein (Pharmingen, San Diego, CA). The mAb FN-18-FITC, which recognizes the monkey CD3 molecule, was also used (Biosource International, Camarillo, CA). Absolute CD4 and CD8 cell counts were measured with TruCount absolute count tubes (Becton Dickinson, Mountainview, CA) according to the manufacturer's instructions. Flow cytometry analysis was performed with the FACS Calibur.
29. J. M. Harouse and C. Cheng-Mayer, unpublished data.
30. H. Deng, D. Unutmaz, V. N. Kewal-Ramani, D. R. Littman, Nature 388, 296 (1997); M. Farzan et al., J. Exp. Med. 186, 405 (1997); G. Alkhatib et al., Nature 388, 238 (1997).
31. H. Deng, D. Unutmaz, V. N. Kewal-Ramani, D. R. Littman, Nature 388, 296 (1997); M. Farzan et al., J. Exp. Med. 186, 405 (1997); G. Alkhatib et al., Nature 388, 238 (1997).
32. H. Deng, D. Unutmaz, V. N. Kewal-Ramani, D. R. Littman, Nature 388, 296 (1997); M. Farzan et al., J. Exp. Med. 186, 405 (1997); G. Alkhatib et al., Nature 388, 238 (1997).
33. J. M. Harouse et al., Virology 248, 95 (1998).
34. R. S. Veazey et al., Science 280, 427 (1998); J. J. Mattapallil et al., J. Virol. 72, 6421 (1998); Z. Smit-McBride et al., J. Virol. 72, 6646 (1998).
35. R. S. Veazey et al., Science 280, 427 (1998); J. J. Mattapallil et al., J. Virol. 72, 6421 (1998); Z. Smit-McBride et al., J. Virol. 72, 6646 (1998).
36. R. S. Veazey et al., Science 280, 427 (1998); J. J. Mattapallil et al., J. Virol. 72, 6421 (1998); Z. Smit-McBride et al., J. Virol. 72, 6646 (1998).
37. Jejunal resectioning and anastomosis were performed by excising about 20 cm of intestinal tissue [D. Slatter, Textbook of Small Animal Surgery (Saunders, Philadelphia, ed. 2, 1993), vol. 1]. We dissected Colonic nodes from colonic mesentery and took ileal wedge biopsies from the antimesenteric side of the intestine followed by closure with a nylon suture. Surgical samples were processed within 30 min of the procedure. All surgical procedures were performed on anesthetized animals and were approved by IACUC.
38. + jejunal LPLs in normal adult rhesus macaques was 34.3% to 56.9%, with a corresponding CD4-CD8 ratio of 0.88 ± 0.22 (n = 4).
39. T. Schneider et al., Gut 37, 524 (1995).
40. C. Bleul, L. Wu, J. A. Hoxie, T. A. Springer, C. R. Mackay, Proc. Natl. Acad. Sci. U.S.A 94, 1925 (1997); R. D. Berkowitz, K. P. Beckerman, T. J. Schall, J. M. McCune, J. Immunol. 161, 3702 (1998).
41. C. Bleul, L. Wu, J. A. Hoxie, T. A. Springer, C. R. Mackay, Proc. Natl. Acad. Sci. U.S.A 94, 1925 (1997); R. D. Berkowitz, K. P. Beckerman, T. J. Schall, J. M. McCune, J. Immunol. 161, 3702 (1998).
42. J. A. Zack et al., Cell 61, 213 (1990).
43. P. Trivedi et al., J. Virol. 70, 6876 (1996); T. Zhu et al., ibid., p. 3098.
44. P. Trivedi et al., J. Virol. 70, 6876 (1996); T. Zhu et al., ibid., p. 3098.
45. Supported in part by National Institutes of Health grants AI41945 and CA72822. We thank D. D. Ho, R. Connor, R. Liu, S. Monard and J. Segal (Aaron Diamond AIDS Research Center); R. Bohm, L. Martin, M. Ratterree, and the animal care staff at Tulane Regional Primate Research Center, R. Koup (University of Texas, Southwestern); P. J. Dailey, L. Sawyer, and J. Booth (Chiron Diagnostics); and R. Veazey (New England Regional Primate Research Center).