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Volumn 284, Issue 5418, 1999, Pages 1354-1356

BRCA1 inhibition of estrogen receptor signaling in transfected cells

Author keywords

[No Author keywords available]

Indexed keywords

ESTROGEN RECEPTOR;

EID: 0033591295     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.284.5418.1354     Document Type: Article
Times cited : (436)

References (24)
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    • note
    • The wild-type BRCA1 expression plasmid (wtBRCA1) was created by cloning the BRCA1 cDNA into the pcDNA3 vector (Invitrogen) with artificially engineered 5′ Hin dIII and 3′ Not I sites. For transcription assays, we incubated asynchronously proliferating cells overnight at 50% to 70% of confluency in 24-well dishes with 0.5 μg of each vector (except where otherwise indicated) in serum-free Dulbecco's modified Eagle's medium (DMEM) containing Lipofectamine (Life Technologies). Cells were washed, incubated in serum-free phenolphthalein-free DMEM (0.2 ml per well) with or without 17β-estradiol (1 μM) for another 24 hours, and harvested for luciferase assays. Values are means ± SEM of four replicate wells and are representative of at least three experiments. Results were similar whether ER-α assays were performed in serum-free DMEM or in the presence of 2% charcoal-stripped serum.
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    • note
    • Supported by U.S. Public Health Service (USPHS) grant R01-ES09169 (E.M.R.), the Elsa U. Pardee Cancer Foundation of Michigan (E.M.R.), the Susan G. Komen Breast Cancer Foundation (R.G.P.), and USPHS grant RO1-CA75503 (R.G.P.).


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.