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50 of the same virus stock and monitoring them with virologic assays for several weeks and by histology in one animal on day 4. At necropsy lymphoid and nonlymphoid organs were sampled from various body locations for virus isolation, histology, immunohistochemistry, and in situ hybridization.
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35S-labeled, single-stranded, antisense RNA probe of SIV-mac239 (Lofstrand Labs, Gaithersburg, MD) was obtained in collaboration with S. Gartner (Yale University, New Haven, CT) through the NIH Research Program. Grant support was provided by the NIH (AI 42129 and 40874 to R.M.S., and AI 40877 to M.P.), the German Ministry of Education and Research (BMBF, grant number 01KI-9767/9), the Korber Foundation (Hamburg), and the Fogarty Foundation (RO3TW00792).
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