Replication of HIV-1 in dendritic cell-derived syncytia at the mucosal surface of the adenoid

Science

Volumn 272, Issue 5258, 1996, Pages 115-117

  Frankel, Sarah S ^a   Wenig, Bruce M ^a   Burke, Allen P ^a   Mannan, Poonam ^a   Thompson, Lester D R ^a   Abbondanzo, Susan L ^a   Nelson, Ann M ^a   Pope, Melissa ^b   Steinman, Ralph M ^b  

^a ARMED FORCES INSTITUTE OF PATHOLOGY   (United States)

^b ROCKEFELLER UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CELL MARKER; GAG PROTEIN;

ACQUIRED IMMUNE DEFICIENCY SYNDROME; ADENOID; ADULT; ARTICLE; CLINICAL ARTICLE; CONTROLLED STUDY; DENDRITIC CELL; FEMALE; HUMAN; HUMAN IMMUNODEFICIENCY VIRUS 1; HUMAN TISSUE; MALE; MUCOSA; PRIORITY JOURNAL; PROTEIN EXPRESSION; SYNCYTIUM; T LYMPHOCYTE; VIRUS REPLICATION;

EID: 0029874948     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.272.5258.115     Document Type: Article

References (33)

1. D. D Ho et al , Nature 373, 123 (1995).
2. X. Wei et al., ibid , p 117.
3. K. Tenner Racz et al , Am. J Pathol 123, 9 (1986).
4. P. Biberfeld et al , ibid 125, 436 (1986)
5. K. Tenner Racz et al., AIDS 2, 299 (1988).
6. P U. Cameron, R. L Dawkins, J A Armstrong, E. Bonifacio, Clin. Exp. Immunol. 68, 465 (1987).
7. H. J. Schuurman, W J. Krone, R. Broekhuizen, J. Goudsmit, Am J Pathol 133, 516 (1988).
8. C H Fox et al., J Infect. Dis 164, 1051 (1991)
9. J Embretson et al., Nature 362, 359 (1993).
10. B. Weiser et al., Proc Natl Acad Sci U S.A 87, 3997 (1990).
11. The specimens were negative for microorganism stains (Brown-Hopps tissue Gram stain, periodic acid-Schiff stain, Grocott's methenamine silver stain, Ziehl-Neelsen acid-fast stain) and for immunolabeling with antibodies to Epstein-Barr virus, herpes simplex virus, or cytomegalovirus.
12. Formalin-fixed, paraffin-embedded tissues were sectioned, stained with the Kal-1 monoclonal antibody to HIV-1 p24 (DAKO) followed by peroxidase-avidin-biotin complex, and counterstained with hematoxylin.
13. K Tenner Racz, P Racz, M Dietrich, P. Kern, Lancet i, 105 (1985), J. A. Armstrong and R. Home, ibid. ii, 370 (1984), G. Pantaleo et al , Nature 362, 355 (1993)
14. K Tenner Racz, P Racz, M Dietrich, P. Kern, Lancet i, 105 (1985), J. A. Armstrong and R. Home, ibid. ii, 370 (1984), G. Pantaleo et al , Nature 362, 355 (1993)
15. K Tenner Racz, P Racz, M Dietrich, P. Kern, Lancet i, 105 (1985), J. A. Armstrong and R. Home, ibid. ii, 370 (1984), G. Pantaleo et al , Nature 362, 355 (1993)
16. M Pope et al., Cell 78, 389 (1994), M. Pope, S Gezelter, N. Gallo, L Hoffman, R M Steinman, J. Exp. Med. 182, 2045 (1995)
17. M Pope et al., Cell 78, 389 (1994), M. Pope, S Gezelter, N. Gallo, L Hoffman, R M Steinman, J. Exp. Med. 182, 2045 (1995)
18. Keratin was identified with two specific monoclonal antibodies followed by alkaline phosphatase-conjugated secondary antibodies (DAKO). Antigens reactive with a monoclonal antibody to p24 and rabbit polyclonal antibodies to S100 (DAKO) were detected with a peroxidase reaction product
19. K. Takahashi et al., Am. J. Pathol 116, 497 (1984), H J. Kahn, A Marks, H Thom, R Baumal, Am J. Clin. Pathol 79, 341 (1983).
20. K. Takahashi et al., Am. J. Pathol 116, 497 (1984), H J. Kahn, A Marks, H Thom, R Baumal, Am J. Clin. Pathol 79, 341 (1983).
21. J. Klein, Immunology (Blackwell, Boston, 1990), p 52, D. W. Fawcett, A Textbook of Histology (Saunders, Philadelphia, 1986)
22. J. Klein, Immunology (Blackwell, Boston, 1990), p 52, D. W. Fawcett, A Textbook of Histology (Saunders, Philadelphia, 1986)
23. L. P. Ruco et al , J. Pathol 176, 391 (1995).
24. G Mosialos et al., Am. J. Pathol. 148, 593 (1996).
25. S. S Frankel et al , unpublished data.
26. D C. Kalter et al , J Immunol. 146, 3396 (1991); J Kanitakis et al , AIDS Res. Hum. Retroviruses 5, 293 (1989) ; H Muller et al , Res Virol. 144, 59 (1993); A Gianetti et al., J. AIDS 6, 329 (1993) .
27. D C. Kalter et al , J Immunol. 146, 3396 (1991); J Kanitakis et al , AIDS Res. Hum. Retroviruses 5, 293 (1989) ; H Muller et al , Res Virol. 144, 59 (1993); A Gianetti et al., J. AIDS 6, 329 (1993) .
28. D C. Kalter et al , J Immunol. 146, 3396 (1991); J Kanitakis et al , AIDS Res. Hum. Retroviruses 5, 293 (1989) ; H Muller et al , Res Virol. 144, 59 (1993); A Gianetti et al., J. AIDS 6, 329 (1993) .
29. D C. Kalter et al , J Immunol. 146, 3396 (1991); J Kanitakis et al , AIDS Res. Hum. Retroviruses 5, 293 (1989) ; H Muller et al , Res Virol. 144, 59 (1993); A Gianetti et al., J. AIDS 6, 329 (1993) .
30. M. Pope, M G. H Betjes, H. Hirmand, L. Hoffman. R. M. Steinman J Invest. Dermatol. 104, 11 (1995).
31. S. Jurriaans et al., Virology 204, 223 (1994)
32. A I Spira et al , J. Exp Med. 183, 215 (1996)
33. Supported by NIH grant AI24775, the Dorothy Schiff Foundation, the Norman and Rosita Winston Fellowship Program (M.P.), the Direct Effect AIDS Researcher Program, Army research and development contract 90MM0604, and an American Registry of Pathology research grant We thank R Virmani for encouragement. The opinions and assertions contained herein are the private views of the authors and are not to be construed as official or as reflecting the views of the Departments of the Navy, Army, or Defense