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Volumn 272, Issue 5267, 1996, Pages 1486-1489

Infection and AIDS in adult macaques after nontraumatic oral exposure to cell-free SIV

Author keywords

[No Author keywords available]

Indexed keywords

ACQUIRED IMMUNE DEFICIENCY SYNDROME; ANIMAL EXPERIMENT; ANIMAL MODEL; ARTICLE; CONTROLLED STUDY; HUMAN; HUMAN CELL; HUMAN IMMUNODEFICIENCY VIRUS 1; MACACA; NONHUMAN; PRIORITY JOURNAL; RISK FACTOR; SEXUAL BEHAVIOR; SEXUAL INTERCOURSE; SIMIAN IMMUNODEFICIENCY VIRUS; VIRUS TRANSMISSION;

EID: 0029896211     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.272.5267.1486     Document Type: Article
Times cited : (126)

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    • note
    • To expose seven young adult macaques [M. mulatta; seronegative for SIV, simian retrovirus type D, and simian T lymphotropic virus type I; median age at inoculation 37 months (range 31 to 41 months)] orally to cell-free SIV, animals were allowed to drink water ad libitum, without food, during the night before exposure. Immediately before inoculation, macaques were sedated with ketamine and underwent a careful exam of the oral cavity, and the virus inoculum (diluted with medium consisting of RPMI 1640, 15% fetal bovine serum, penicillin-streptomycin, and 1-glutamine to achieve a total volume of 3.0 ml) was dripped without trauma onto the back of the tongue.
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    • All animal experiments were approved by the Animal Care and Use Committees at the Tulane Regional Primate Research Center and at the Dana-Farber Cancer Institute, which take responsibility for humane care and use of laboratory animals. We are committed to comply with the Principles for Use of Animals, the Guide for the Care and Use of Laboratory Animals, the Provisions of the Animal Welfare Act, and other applicable laws and regulations. The center's statement of assurance is on file with the USPHS, Office for Protection from Research Risks. These facilities are accredited by the American Association for Accreditation of Laboratory Animal Care. Animals are anesthetized with ketamine before all procedures that require the removal of animals from their cages. No restraining devices are necessary during these procedures. When necessary, moribund animals are euthanized by iv inoculation of a lethal dose of sodium pentobarbital.
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    • note
    • We thank L. Corey for sharing unpublished data; A. Rosowsky for reviewing the manuscript; C. Lanclos, E. deHaro, E. Pecunia, and S. Kraus for technical assistance; and L. Geronimo for preparing the manuscript. Supported in part by NIH grants U01 AI24345, RO1-AI32330, RO1-AI34266, and RO1-AI35533 to R.M.R.; RR-00164, providing base support for the Tulane Regional Primate Research Center; AI-15093 and AI-35550 to M.M.-C.; and AI-15119 to L.N.M.; and by the Center for AIDS Research Core Grant no. IP30 28691 awarded to the Dana-Farber Cancer Institute as support for the iInstitute's AIDS research efforts. T.W.B. is a Scholar of the Pediatric AIDS Foundation and A.M.T. is a Fellow of the Minnie Burke Foundation.


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