Complex mechanisms underlying impaired activation of Cdk4 and Cdk2 in replicative senescence: Roles of p16, p21, and cyclin D1

Experimental Cell Research

Volumn 253, Issue 2, 1999, Pages 503-510

  Morisaki, Hirobumi ^a,b   Ando, Akikazu ^b   Nagata, Yoshiho ^b   Pereira Smith, Olivia ^c   Smith, James R ^c   Ikeda, Kyoji ^a   Nakanishi, Makoto ^a,d  

^a NATIONAL CENTER FOR GERIATRICS AND GERONTOLOGY   (Japan)

^b CHIBA UNIVERSITY   (Japan)

^c BAYLOR COLLEGE OF MEDICINE   (United States)

^d NAGOYA CITY UNIV MEDICAL SCHOOL   (Japan)

Author keywords

CAK; Cdk inhibitor; Cdk2; Cdk4; Cyclin D1; Cyclin E; Senescence

Indexed keywords

CYCLIN D1; CYCLIN DEPENDENT KINASE; CYCLIN E; MITOGENIC AGENT; PROTEIN P16; PROTEIN P21;

ARTICLE; CELL AGING; CELL CYCLE; CELL DIVISION; COMPLEX FORMATION; CONTROLLED STUDY; ENZYME ACTIVATION; ENZYME INHIBITION; FIBROBLAST; GENE EXPRESSION; HUMAN; HUMAN CELL; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN INTERACTION; REGULATORY MECHANISM;

EID: 0033573113     PISSN: 00144827     EISSN: None     Source Type: Journal    
DOI: 10.1006/excr.1999.4698     Document Type: Article

References (33)

1. Hayflick L., Moorhead P. S. The serial cultivation of human diploid cell strains. Exp. Cell Res. 25:1961;585-621.
2. Hayflick L. The limited in vitro lifetime of human diploid cell strains. Exp. Cell Res. 37:1965;614-635.
3. Grove G. L., Cristofalo V. J. Characterization of the cell cycle of cultured human diploid cells: Effects of aging and hydrocortisone. J. Cell Physiol. 90:1977;415-422.
4. Lucibello F. C., Sewing A., Bruesselbach S., Buerger C., Mueller R. Deregulation of cyclins D1 and E and suppression of cdk2 and cdk4 in senescent human fibroblasts. J. Cell. Sci. 105:1993;123-133.
5. Stein G. H., Besson M., Gordon L. Failure to phosphorylate the retinoblastoma gene product in senescent human fibroblasts. Science. 249:1990;666-669.
6. Stein G. H., Drullinger L. F., Robetorye R. S., Pereira-Smith O. M., Smith J. R. Senescent cells fail to express cdc2, cycA, and cycB in response to mitogen stimulation. Proc. Natl. Acad. Sci. 88:1991;11012-11016.
7. Chang C.-D., Phillips P., Lipson K. E., Cristofalo V. J., Baserga R. Senescent human fibroblasts have a post-transcriptional block in the expression of the proliferating cell nuclear antigen gene. J. Biol. Chem. 266:1991;8663-8666.
8. Sherr C. J. G1 phase progression: Cycling on cue. Cell. 79:1994;551-555.
9. Hunter T., Pines J. Cyclins and cancer. Cell. 79:1994;573-582.
10. Morgan D. O. Principles of CDK regulation. Nature. 374:1995;131-134.
11. Sherr C. J., Roberts J. M. Inhibitors of mammalian G1 cyclin-dependent kinases. Genes Dev. 9:1995;1149-1163.
12. Smith J. R., Pereira-Smith O. M. Replicative senescence: Implications for in vivo aging and tumor suppression. Science. 273:1996;63-67.
13. Noda A., Ning Y., Venable S. F., Pereira-Smith O. M., Smith J. R. Cloning of senescent cell-derived inhibitors of DNA synthesis using an expression screen. Exp. Cell Res. 211:1994;90-98.
14. Hara E., Smith R., Parry D., Tahara H., Stone S., Peters G. Regulation of p16CDKN2 expression and its implications for cell immortalization and senescence. Mol. Cell. Biol. 16:1996;859-867.
15. Dulic V., Drullinger L. F., Lees E., Reed S. I., Stein G. H. Altered regulation of G1 cyclins in senescent human diploid fibroblasts: Accumulation of inactive cyclin E-Cdk2 and cyclin D1-Cdk2 complexes. Proc. Natl. Acad. Sci. USA. 90:1993;11034-11038.
16. Morisaki H., Fujimoto A., Ando A., Nagata Y., Ikeda K., Nakanishi M. Cell cycle-dependent phosphorylation of p27 cyclin-dependent kinase (Cdk) inhibitor by cyclin E/Cdk2. Biochem. Biophys. Res. Commun. 240:1997;386-390.
17. Parry D., Bates S., Mann D. J., Peters G. Lack of cyclin D-Cdk complexes in Rb-negative cells correlates with high levels of p16INK4/MTS1 tumour suppressor gene product. EMBO J. 14:1995;503-511.
18. Serrano M., Hannon G. J., Beach D. A new regulatory motif in cell-cycle control causing specific inhibition of cyclin D/CDK4. Nature. 366:1993;704-707.
19. Aprelikova O., Xiong Y., Liu E. T. Both p16 and p21 families of cyclin-dependent kinase (CDK) inhibitors block the phosphorylation of cyclin-dependent kinases by the CDK-activating kinase. J. Biol. Chem. 270:1995;18195-18197.
20. Ohtsubo M., Roberts J. M. Cyclin-dependent regulation of G1 in mammalian fibroblasts. Science. 259:1993;1908-1912.
21. Labaer J., Garrett M. D., Stevenson L. F., Slingerland J. M., Sandhu C., Chou H. S., Fattaey A., Harlow E. New functional activities for the p21 family of CDK inhibitors. Genes Dev. 11:1997;847-862.
22. Russo A. A., Jeffrey P. D., Pattern A. K., Massague J., Pavletich N. P. Crystal structure of the p27Kip1 cyclin-dependent-kinase inhibitor bound to the cyclinA-Cdk2 complex. Nature. 382:1996;325-331.
23. Luh F. Y., Archer S. J., Domaille P. J., Smith B. O., Owen D., Brotherton D. H., Raine A. R. C., Xu X., Brizuela L., Brenner S. L., Laute E. D. Structure of the cyclin-dependent kinase inhibitor p19Ink4d. Nature. 389:1997;999-1003.
24. INK4a inhibits both CDK4- and CDK6-associated kinase activity by reassortment of cyclin-CDK-inhibitor complexes. Mol. Cell. Biol. 19:1999;1981-1989.
25. INK4a. Mol. Cell. Biol. 19:1999;3916-3928.
26. Hirai H., Roussel M. F., Kato J.-Y., Ashmun R. A., Sherr C. J. Novel INK4 proteins, p19 and p18, are specific inhibitors of the cyclin D-dependent kinases CDK4 and CDK6. Mol. Cell. Biol. 15:1995;2672-2681.
27. Reynisdottir I., Massague J. The subcellular locations of p15(Ink4b) and p27(Kip1) coordinate their inhibitory interactions with cdk4 and cdk2. Genes Dev. 11:1997;492-503.
28. INK4a. Nature. 395:1998;237-243.
29. Jeffrey P. D., Russo A. A., Polyak K., Gibbs E., Hurwitz J., Massague J., Pavletich N. P. Mechanism of CDK activation revealed by the structure of a cyclinA-CDK2 complex. Nature. 376:1995;313-320.
30. Alcorta D. A., Xiong Y., Phelps D., Hannon G., Beach D., Barrett J. C. Involvement of the cyclin-dependent kinase inhibitor p16 (INK4a) in replicative senescence of normal human fibroblasts. Proc. Natl. Acad. Sci. USA. 93:1996;13742-13747.
31. Stein G. H., Drullinger L. F., Soulard A., Dulic V. Differential roles for cyclin-dependent kinase inhibitors p21 and p16 in the mechanisms of senescence and differentiation in human fibroblasts. Mol. Cell. Biol. 19:1999;2109-2117.
32. Kato J-Y., Matsuoka M., Polyak K., Massague J., Sherr C. J. Cyclic AMP-induced G1 phase arrest mediated by an inhibitor (p27Kip1) of cyclin-dependent kinase 4 activation. Cell. 79:1994;487-496.
33. Kato A., Takahashi H., Takahashi Y., Matsushime H. Inactivation of the cyclin D-dependent kinase in the rat fibroblast cell line, 3Y1, induced by contact inhibition. J. Biol. Chem. 272:1997;8065-8070.