메뉴 건너뛰기




Volumn 283, Issue 5408, 1999, Pages 1739-1742

Negative regulation of wingless signaling by D-Axin, a Drosophila homolog of Axin

Author keywords

[No Author keywords available]

Indexed keywords

AXIN; BETA CATENIN; CASEIN KINASE; CELL PROTEIN; RNA; UNCLASSIFIED DRUG;

EID: 0033548495     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.283.5408.1739     Document Type: Article
Times cited : (176)

References (42)
  • 1
    • 0028447438 scopus 로고
    • E. Siegfried and N. Perrimon, Bioessays 16, 395 (1994); K. M. Cadigan and R. Nusse, Genes Dev. 11, 3286 (1997).
    • (1994) Bioessays , vol.16 , pp. 395
    • Siegfried, E.1    Perrimon, N.2
  • 2
    • 0031456158 scopus 로고    scopus 로고
    • E. Siegfried and N. Perrimon, Bioessays 16, 395 (1994); K. M. Cadigan and R. Nusse, Genes Dev. 11, 3286 (1997).
    • (1997) Genes Dev. , vol.11 , pp. 3286
    • Cadigan, K.M.1    Nusse, R.2
  • 3
    • 0029964851 scopus 로고    scopus 로고
    • J. R. Miller and R. T. Moon, Genes Dev. 10, 2527 (1996); K. Willert and R. Nusse, Curr. Opin. Genet. Dev. 8, 95 (1998).
    • (1996) Genes Dev. , vol.10 , pp. 2527
    • Miller, J.R.1    Moon, R.T.2
  • 5
    • 0030592517 scopus 로고    scopus 로고
    • K. W. Kinzler and B. Vogelstein, Cell 87, 159 (1996); P. Polakis, Biochim. Biophys. Acta 1332, F127 (1997).
    • (1996) Cell , vol.87 , pp. 159
    • Kinzler, K.W.1    Vogelstein, B.2
  • 6
    • 0031557898 scopus 로고    scopus 로고
    • K. W. Kinzler and B. Vogelstein, Cell 87, 159 (1996); P. Polakis, Biochim. Biophys. Acta 1332, F127 (1997).
    • (1997) Biochim. Biophys. Acta , vol.1332
    • Polakis, P.1
  • 7
    • 0030949463 scopus 로고    scopus 로고
    • P. J. Morin et al., Science 275, 1787 (1997); B. Rubinfeld et al., ibid., p. 1790.
    • (1997) Science , vol.275 , pp. 1787
    • Morin, P.J.1
  • 8
    • 0030949463 scopus 로고    scopus 로고
    • P. J. Morin et al., Science 275, 1787 (1997); B. Rubinfeld et al., ibid., p. 1790.
    • Science , pp. 1790
    • Rubinfeld, B.1
  • 9
    • 0030801002 scopus 로고    scopus 로고
    • 6 clones of a Drosophila embryo cDNA library fused to the transcription activation domain of yeast GAL4 in the vector pGAD10 and isolated three independent partial clones of the d-axin cDNA. A larger partial d-axin cDNA clone (2.7 kb) was isolated by rescreening a λgt11 Drosophila embryo cDNA library with the smaller cDNAs, and the remaining 5′-end region was obtained by the 5′ RACE (rapid amplification of cDNA ends) technique (Clontech). Multiple independent polymerase; chain reaction (PCR) products were analyzed to exclude the possibility of PCR-induced errors. Sequence comparison analysis was performed with the blastp program [S. F. Altschul et al., Nucleic Acids Res. 25, 3389 (1997)].
    • (1997) Nucleic Acids Res. , vol.25 , pp. 3389
    • Altschul, S.F.1
  • 10
    • 0031457978 scopus 로고    scopus 로고
    • L. Zeng et al., Cell 90, 181 (1997).
    • (1997) Cell , vol.90 , pp. 181
    • Zeng, L.1
  • 11
    • 0032562603 scopus 로고    scopus 로고
    • J. Behrens et al., Science 280, 596 (1998).
    • (1998) Science , vol.280 , pp. 596
    • Behrens, J.1
  • 12
    • 0032473355 scopus 로고    scopus 로고
    • S. Ikeda et al., EMBO J. 17, 1371 (1998); C. Sakanaka, J. B. Weiss, L. T. Williams, Proc. Natl. Acad. Sci. U.S.A. 95, 3020 (1998); H. Yamamoto et al., Mol. Cell. Biol. 18, 2867 (1998); S. Kishida et al., J. Biol. Chem. 273, 10823 (1998); K. Itoh, V. E. Krupnik, S. Y. Sokol, Curr. Biol. 8, 591 (1998); M. J. Hart, R. de los Santos, I. N. Albert, B. Rubinfeld, P. Polakis, ibid., p. 573.
    • (1998) EMBO J. , vol.17 , pp. 1371
    • Ikeda, S.1
  • 13
    • 0032539945 scopus 로고    scopus 로고
    • S. Ikeda et al., EMBO J. 17, 1371 (1998); C. Sakanaka, J. B. Weiss, L. T. Williams, Proc. Natl. Acad. Sci. U.S.A. 95, 3020 (1998); H. Yamamoto et al., Mol. Cell. Biol. 18, 2867 (1998); S. Kishida et al., J. Biol. Chem. 273, 10823 (1998); K. Itoh, V. E. Krupnik, S. Y. Sokol, Curr. Biol. 8, 591 (1998); M. J. Hart, R. de los Santos, I. N. Albert, B. Rubinfeld, P. Polakis, ibid., p. 573.
    • (1998) Proc. Natl. Acad. Sci. U.S.A. , vol.95 , pp. 3020
    • Sakanaka, C.1    Weiss, J.B.2    Williams, L.T.3
  • 14
    • 0031943002 scopus 로고    scopus 로고
    • S. Ikeda et al., EMBO J. 17, 1371 (1998); C. Sakanaka, J. B. Weiss, L. T. Williams, Proc. Natl. Acad. Sci. U.S.A. 95, 3020 (1998); H. Yamamoto et al., Mol. Cell. Biol. 18, 2867 (1998); S. Kishida et al., J. Biol. Chem. 273, 10823 (1998); K. Itoh, V. E. Krupnik, S. Y. Sokol, Curr. Biol. 8, 591 (1998); M. J. Hart, R. de los Santos, I. N. Albert, B. Rubinfeld, P. Polakis, ibid., p. 573.
    • (1998) Mol. Cell. Biol. , vol.18 , pp. 2867
    • Yamamoto, H.1
  • 15
    • 0032079884 scopus 로고    scopus 로고
    • S. Ikeda et al., EMBO J. 17, 1371 (1998); C. Sakanaka, J. B. Weiss, L. T. Williams, Proc. Natl. Acad. Sci. U.S.A. 95, 3020 (1998); H. Yamamoto et al., Mol. Cell. Biol. 18, 2867 (1998); S. Kishida et al., J. Biol. Chem. 273, 10823 (1998); K. Itoh, V. E. Krupnik, S. Y. Sokol, Curr. Biol. 8, 591 (1998); M. J. Hart, R. de los Santos, I. N. Albert, B. Rubinfeld, P. Polakis, ibid., p. 573.
    • (1998) J. Biol. Chem. , vol.273 , pp. 10823
    • Kishida, S.1
  • 16
    • 0032493042 scopus 로고    scopus 로고
    • S. Ikeda et al., EMBO J. 17, 1371 (1998); C. Sakanaka, J. B. Weiss, L. T. Williams, Proc. Natl. Acad. Sci. U.S.A. 95, 3020 (1998); H. Yamamoto et al., Mol. Cell. Biol. 18, 2867 (1998); S. Kishida et al., J. Biol. Chem. 273, 10823 (1998); K. Itoh, V. E. Krupnik, S. Y. Sokol, Curr. Biol. 8, 591 (1998); M. J. Hart, R. de los Santos, I. N. Albert, B. Rubinfeld, P. Polakis, ibid., p. 573.
    • (1998) Curr. Biol. , vol.8 , pp. 591
    • Itoh, K.1    Krupnik, V.E.2    Sokol, S.Y.3
  • 17
    • 0032473355 scopus 로고    scopus 로고
    • S. Ikeda et al., EMBO J. 17, 1371 (1998); C. Sakanaka, J. B. Weiss, L. T. Williams, Proc. Natl. Acad. Sci. U.S.A. 95, 3020 (1998); H. Yamamoto et al., Mol. Cell. Biol. 18, 2867 (1998); S. Kishida et al., J. Biol. Chem. 273, 10823 (1998); K. Itoh, V. E. Krupnik, S. Y. Sokol, Curr. Biol. 8, 591 (1998); M. J. Hart, R. de los Santos, I. N. Albert, B. Rubinfeld, P. Polakis, ibid., p. 573.
    • Curr. Biol. , pp. 573
    • Hart, M.J.1    De Los Santos, R.2    Albert, I.N.3    Rubinfeld, B.4    Polakis, P.5
  • 19
    • 0344556823 scopus 로고    scopus 로고
    • note
    • 35S-methionine with the coupled transcription-translation TNT system (Promega). CST and GST-fusion proteins (2 μg) immobilized to glutathione-Sepharose beads were mixed with in vitro-translated proteins in binding buffer [20 mM tris-HCl (pH 8.0), 140 mM NaCl, 1 mM EDTA, 1 mM dithiothreitol, 10% glycerol, 1% Triton X-100, and 10 μg/ml each of aprotinin, leupeptin, and pepstatin) for 1 hour at 4°C. After washing five times with binding buffer, bound proteins were fractionated by SDS-polyacrylamide gel electrophoresis (PAGE) followed by autoradiography.
  • 20
    • 0344987756 scopus 로고    scopus 로고
    • unpublished data
    • F. Hamada et al., unpublished data.
    • Hamada, F.1
  • 22
    • 0344125270 scopus 로고    scopus 로고
    • note
    • Using in situ hybridization to polytene chromosomes, we mapped the d-axin gene to 99D in the third chromosome. The genomic P1 clone DS00026 from the Berkeley Drosophila Genome Project contained the genomic region corresponding to the d-axin cDNA. The genomic organization of the d-axin locus and the intron-exon structure were determined by Southern (DNA) blot analysis and subsequent sequencing across the exon-intron boundaries.
  • 23
    • 0023832435 scopus 로고
    • p, Δ2-3 strain [H. M. Robertson et al., Genetics 118, 461 (1988)].
    • (1988) Genetics , vol.118 , pp. 461
    • Robertson, H.M.1
  • 24
    • 0027160708 scopus 로고
    • The GAL4/UAS system was used for overexpression of d-axin [A. H. Brand and N. Perrimon, Development 118, 401 (1993)]. The entire d-axin open reading frames of various constructs were subcloned into the pUAST vector and introduced into the w germ line by the standard P-element transformation method These UAS-d-axin lines were crossed with several GAL4 drivers [ Y. Shiga, M. Tanaka-Matakatsu, S. Hayashi, Dev. Growth Differ. 38, 99 (1996); E. J. Rulifson, C. A. Micchelle, J. D. Axelrod, N. Perrimon, S. S. Blair, Nature 384, 72 (1996)].
    • (1993) Development , vol.118 , pp. 401
    • Brand, A.H.1    Perrimon, N.2
  • 25
    • 0030028059 scopus 로고    scopus 로고
    • The GAL4/UAS system was used for overexpression of d-axin [A. H. Brand and N. Perrimon, Development 118, 401 (1993)]. The entire d-axin open reading frames of various constructs were subcloned into the pUAST vector and introduced into the w germ line by the standard P-element transformation method These UAS-d-axin lines were crossed with several GAL4 drivers [ Y. Shiga, M. Tanaka-Matakatsu, S. Hayashi, Dev. Growth Differ. 38, 99 (1996); E. J. Rulifson, C. A. Micchelle, J. D. Axelrod, N. Perrimon, S. S. Blair, Nature 384, 72 (1996)].
    • (1996) Dev. Growth Differ. , vol.38 , pp. 99
    • Shiga, Y.1    Tanaka-Matakatsu, M.2    Hayashi, S.3
  • 26
    • 0029904837 scopus 로고    scopus 로고
    • The GAL4/UAS system was used for overexpression of d-axin [A. H. Brand and N. Perrimon, Development 118, 401 (1993)]. The entire d-axin open reading frames of various constructs were subcloned into the pUAST vector and introduced into the w germ line by the standard P-element transformation method These UAS-d-axin lines were crossed with several GAL4 drivers [ Y. Shiga, M. Tanaka-Matakatsu, S. Hayashi, Dev. Growth Differ. 38, 99 (1996); E. J. Rulifson, C. A. Micchelle, J. D. Axelrod, N. Perrimon, S. S. Blair, Nature 384, 72 (1996)].
    • (1996) Nature , vol.384 , pp. 72
    • Rulifson, E.J.1    Micchelle, C.A.2    Axelrod, J.D.3    Perrimon, N.4    Blair, S.S.5
  • 27
    • 0030300085 scopus 로고    scopus 로고
    • Germ-line clones of d-axin were generated by use of the FLP-DFS technique [T. B. Chou and N. Perrimon, Genetics 144, 1673 (1996)]. All embryos derived from homozygous d-axin germ-line clones die during embryonic development. The TM3 gfp chromosome was used to determine the zygotic genotype of embryos. Cuticles were prepared and mounted in Hoyer's medium [J. van der Meer, Drosophila Inf. Serv. 52, 160 (1977)], viewed, and photographed under dark field.
    • (1996) Genetics , vol.144 , pp. 1673
    • Chou, T.B.1    Perrimon, N.2
  • 28
    • 0030300085 scopus 로고    scopus 로고
    • Germ-line clones of d-axin were generated by use of the FLP-DFS technique [T. B. Chou and N. Perrimon, Genetics 144, 1673 (1996)]. All embryos derived from homozygous d-axin germ-line clones die during embryonic development. The TM3 gfp chromosome was used to determine the zygotic genotype of embryos. Cuticles were prepared and mounted in Hoyer's medium [J. van der Meer, Drosophila Inf. Serv. 52, 160 (1977)], viewed, and photographed under dark field.
    • (1977) Drosophila Inf. Serv. , vol.52 , pp. 160
    • Van Der Meer, J.1
  • 30
    • 0030606264 scopus 로고    scopus 로고
    • E. Siegfried, E. L. Wilder, N. Perrimon, Nature 367, 76 (1994); M. Zecca, K. Basler, G. Struhl. Cell 87, 833 (1996).
    • (1996) Cell , vol.87 , pp. 833
    • Zecca, M.1    Basler, K.2    Struhl, G.3
  • 32
    • 0027267041 scopus 로고
    • + for imaginal discs [J. Jiang and G. Struhl, Nature 391, 493 (1998)]. Mutant phenotypes were induced in the mid-second instar larval stage by heat shock at 37°C for 30 min. For the induction of the hip70-myc-gfp gene, wandering larvae were heat shocked at 37°C for 60 min and then processed for immunostaining after a 60-min recovery period at 25°C. The mutant clones can be recognized by the y marker in adults and by the absence of GFP expression in the imaginal discs, respectively.
    • (1993) Development , vol.117 , pp. 1223
    • Xu, T.1    Rubin, G.M.2
  • 33
    • 0032576777 scopus 로고    scopus 로고
    • + for imaginal discs [J. Jiang and G. Struhl, Nature 391, 493 (1998)]. Mutant phenotypes were induced in the mid-second instar larval stage by heat shock at 37°C for 30 min. For the induction of the hip70-myc-gfp gene, wandering larvae were heat shocked at 37°C for 60 min and then processed for immunostaining after a 60-min recovery period at 25°C. The mutant clones can be recognized by the y marker in adults and by the absence of GFP expression in the imaginal discs, respectively.
    • (1998) Nature , vol.391 , pp. 493
    • Jiang, J.1    Struhl, G.2
  • 34
    • 0029795757 scopus 로고    scopus 로고
    • W. J. Brook and S. M. Cohen, Science 273, 1373 (1996); J. Jiang and G. Struhl, Cell 86, 401 (1996); A. Penton and F. M. Hoffmann, Nature 382, 162 (1996).
    • (1996) Science , vol.273 , pp. 1373
    • Brook, W.J.1    Cohen, S.M.2
  • 35
    • 0030576535 scopus 로고    scopus 로고
    • W. J. Brook and S. M. Cohen, Science 273, 1373 (1996); J. Jiang and G. Struhl, Cell 86, 401 (1996); A. Penton and F. M. Hoffmann, Nature 382, 162 (1996).
    • (1996) Cell , vol.86 , pp. 401
    • Jiang, J.1    Struhl, G.2
  • 36
    • 0029901698 scopus 로고    scopus 로고
    • W. J. Brook and S. M. Cohen, Science 273, 1373 (1996); J. Jiang and G. Struhl, Cell 86, 401 (1996); A. Penton and F. M. Hoffmann, Nature 382, 162 (1996).
    • (1996) Nature , vol.382 , pp. 162
    • Penton, A.1    Hoffmann, F.M.2
  • 38
    • 0031020656 scopus 로고    scopus 로고
    • C. J. Neumann, and S. M. Cohen, Development 124, 871 (1997); K. M. Cadigan, M. P. Fish, E. J. Rulifson, R. Nusse, Cell 93, 767 (1998).
    • (1997) Development , vol.124 , pp. 871
    • Neumann, C.J.1    Cohen, S.M.2
  • 40
    • 0028047389 scopus 로고
    • Wing imaginal discs from third instar larvae were dissected, fixed, and stained with mouse antibodies to Arm (anti-Arm, N2-7A1) [M. Peifer, D. Sweeton, M. Casey, E. Wieschaus, Development 120, 369 (1994)] or anti-DII [G. Vachon et al., Cell 71, 437 (1992)]. These antibodies were detected with rhodamine-conjugated goat anti-mouse immunoglobulin G (Jackson Immunochemicals). Confocal fluorescent images were obtained with a Zeiss LSM510 microscope.
    • (1994) Development , vol.120 , pp. 369
    • Peifer, M.1    Sweeton, D.2    Casey, M.3    Wieschaus, E.4
  • 41
    • 0026492021 scopus 로고
    • Wing imaginal discs from third instar larvae were dissected, fixed, and stained with mouse antibodies to Arm (anti-Arm, N2-7A1) [M. Peifer, D. Sweeton, M. Casey, E. Wieschaus, Development 120, 369 (1994)] or anti-DII [G. Vachon et al., Cell 71, 437 (1992)]. These antibodies were detected with rhodamine-conjugated goat anti-mouse immunoglobulin G (Jackson Immunochemicals). Confocal fluorescent images were obtained with a Zeiss LSM510 microscope.
    • (1992) Cell , vol.71 , pp. 437
    • Vachon, G.1
  • 42
    • 0344987755 scopus 로고    scopus 로고
    • note
    • We thank J. Jiang, G. Struhl, the Bloomington Stock Center, and the European Drosophila P-element-induced Lethal Stock Center (Szeged Center) for fly stocks; S. Yanagawa, S. Cohen, and E. Wieschaus for plasmids and antibodies; the Berkeley Drosophila Genome Project and E. Nitasaka for genomic P1 clones; A. Ogai, Y. Katoh, N. Kakinuma, T. Shikina, T. Wada, A. Furui, and M. Ichikawa for technical assistance; and S. Higashijima, E. Shishido, T. Tabata, and K. W. Cho for helpful discussions. Supported by grants from the Ministry of Education, Science, and Culture of Japan and the National Institute for Basic Biology Cooperative Research Program.


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.