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Volumn 121, Issue 35, 1999, Pages 8124-8125

Biosynthesis of Mycarose: Isolation and Characterization of Enzymes Involved in the C-2 Deoxygenation

Author keywords

[No Author keywords available]

Indexed keywords

DEOXYSUGAR; ENZYME; IRON SULFUR PROTEIN; MYCAROSE; OXIDOREDUCTASE; UNCLASSIFIED DRUG;

EID: 0033536504     PISSN: 00027863     EISSN: None     Source Type: Journal    
DOI: 10.1021/ja991713o     Document Type: Letter
Times cited : (59)

References (43)
  • 1
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    • Johnson, D. A.; Liu, H.-w. In The Biology-Chemistry Interface: A Tribute to Koji Nakanishi; Cooper, R., Snyder, J. D., Eds.; Marcel Dekker: New York; 1999, in press.
  • 14
    • 0028236752 scopus 로고
    • It should be pointed out that the tylIBA and tylLM segments of the tyl cluster have also been sequenced by Cundliffe and co-workers (Merson-Davies, L. A.; Cundliffe, E. Mol. Microbiol. 1994, 13, 349-355. Gandecha, A. R.; Large, S. L.; Cundliffe, E. Gene 1997, 184, 197-203. Fish, S. A.; Cundliffe, E. Microbiology 1997, 143, 3871-3876. Bulter, A. R.; Bate, N.; Cundliffe, E. Chem. Biol. 1999, 6, 287-292).
    • (1994) Mol. Microbiol. , vol.13 , pp. 349-355
    • Merson-Davies, L.A.1    Cundliffe, E.2
  • 15
    • 0031013034 scopus 로고    scopus 로고
    • It should be pointed out that the tylIBA and tylLM segments of the tyl cluster have also been sequenced by Cundliffe and co-workers (Merson-Davies, L. A.; Cundliffe, E. Mol. Microbiol. 1994, 13, 349-355. Gandecha, A. R.; Large, S. L.; Cundliffe, E. Gene 1997, 184, 197-203. Fish, S. A.; Cundliffe, E. Microbiology 1997, 143, 3871-3876. Bulter, A. R.; Bate, N.; Cundliffe, E. Chem. Biol. 1999, 6, 287-292).
    • (1997) Gene , vol.184 , pp. 197-203
    • Gandecha, A.R.1    Large, S.L.2    Cundliffe, E.3
  • 16
    • 0031437605 scopus 로고    scopus 로고
    • It should be pointed out that the tylIBA and tylLM segments of the tyl cluster have also been sequenced by Cundliffe and co-workers (Merson-Davies, L. A.; Cundliffe, E. Mol. Microbiol. 1994, 13, 349-355. Gandecha, A. R.; Large, S. L.; Cundliffe, E. Gene 1997, 184, 197-203. Fish, S. A.; Cundliffe, E. Microbiology 1997, 143, 3871-3876. Bulter, A. R.; Bate, N.; Cundliffe, E. Chem. Biol. 1999, 6, 287-292).
    • (1997) Microbiology , vol.143 , pp. 3871-3876
    • Fish, S.A.1    Cundliffe, E.2
  • 17
    • 0033133991 scopus 로고    scopus 로고
    • It should be pointed out that the tylIBA and tylLM segments of the tyl cluster have also been sequenced by Cundliffe and co-workers (Merson-Davies, L. A.; Cundliffe, E. Mol. Microbiol. 1994, 13, 349-355. Gandecha, A. R.; Large, S. L.; Cundliffe, E. Gene 1997, 184, 197-203. Fish, S. A.; Cundliffe, E. Microbiology 1997, 143, 3871-3876. Bulter, A. R.; Bate, N.; Cundliffe, E. Chem. Biol. 1999, 6, 287-292).
    • (1999) Chem. Biol. , vol.6 , pp. 287-292
    • Bulter, A.R.1    Bate, N.2    Cundliffe, E.3
  • 25
    • 85069254809 scopus 로고    scopus 로고
    • 6tag, the N-terminal amino acid sequencing confirmed that the first 10 residues of TylX3 (AHSSAT-AGPQ) and TylC1 (SGMYVQLGR) are identical to the respective translated tylX3 and tylC1 sequences, except for the deletion of the first methionine residue in both cases.
  • 26
    • 85069245090 scopus 로고    scopus 로고
    • The calculated molecular mass for TylX3 is 55 795 Da, and that for TylC1 is 36 920 Da.
  • 27
    • 85069242112 scopus 로고    scopus 로고
    • 2+in TylX3 catalysis must await further investigation.
  • 29
    • 85069243753 scopus 로고    scopus 로고
    • The incubation mixture was loaded on an Adsorbosphere SAX column (5 μm, 4.6 × 250 mm), and a linear gradient from 140 to 320 mM potassium phosphate buffer (pH 3.6) over 20 min was used to elute the reaction products (monitored at 278 nm). Under these conditions, the retention times were 4.8 min for maltol (6), 13.0 min for substrate 3, and 17.6 min for TDP.
  • 30
    • 85069251407 scopus 로고    scopus 로고
    • A preparative incubation contained the RfbB product (3, 14.0 mg, 23.7 μmol) and NADPH (21.7 mg, 26.1 μmol) in 1.5 mL of 100 mM potassium phosphate buffer (pH 7.5). The reaction was initiated by the addition of TylX3 (6.5 nmol) and TylC1 (10.5 nmol). Due to the instability of the TylX3 product, an excess of TylC1 was used. The reaction was incubated at room temperature and was followed by monitoring the consumption of NADPH at 340 nm. The reaction was usually complete within 2 h.
  • 31
    • 85069239979 scopus 로고    scopus 로고
    • The same HPLC conditions as described in ref 16 were used. The retention time for product 5 was 13.8 min.
  • 32
    • 85069250304 scopus 로고    scopus 로고
    • 2O): δ -10.7 (d, J = 21.9 Hz), -12.9 (d, J = 21.9 Hz).
  • 40
    • 7144221518 scopus 로고
    • Boyer, P., Ed.; Academic Press: New York
    • Glaser, L.; Zarkowsky, R. In The Enzymes; Boyer, P., Ed.; Academic Press: New York, 1971; Vol. 5, pp 465-480.
    • (1971) The Enzymes , vol.5 , pp. 465-480
    • Glaser, L.1    Zarkowsky, R.2
  • 41
    • 0005786913 scopus 로고
    • Carbohydrates in Solution; Gould, R., Ed.; American Chemical Society: Washington, DC
    • (b) Gabriel, O. In Carbohydrates in Solution; Gould, R., Ed.; Advances in Chemistry Series 117, American Chemical Society: Washington, DC, 1973; pp 387-410.
    • (1973) Advances in Chemistry Series , vol.117 , pp. 387-410
    • Gabriel, O.1


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.