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It should be pointed out that the tylIBA and tylLM segments of the tyl cluster have also been sequenced by Cundliffe and co-workers (Merson-Davies, L. A.; Cundliffe, E. Mol. Microbiol. 1994, 13, 349-355. Gandecha, A. R.; Large, S. L.; Cundliffe, E. Gene 1997, 184, 197-203. Fish, S. A.; Cundliffe, E. Microbiology 1997, 143, 3871-3876. Bulter, A. R.; Bate, N.; Cundliffe, E. Chem. Biol. 1999, 6, 287-292).
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0031013034
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It should be pointed out that the tylIBA and tylLM segments of the tyl cluster have also been sequenced by Cundliffe and co-workers (Merson-Davies, L. A.; Cundliffe, E. Mol. Microbiol. 1994, 13, 349-355. Gandecha, A. R.; Large, S. L.; Cundliffe, E. Gene 1997, 184, 197-203. Fish, S. A.; Cundliffe, E. Microbiology 1997, 143, 3871-3876. Bulter, A. R.; Bate, N.; Cundliffe, E. Chem. Biol. 1999, 6, 287-292).
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Gandecha, A.R.1
Large, S.L.2
Cundliffe, E.3
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16
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0031437605
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It should be pointed out that the tylIBA and tylLM segments of the tyl cluster have also been sequenced by Cundliffe and co-workers (Merson-Davies, L. A.; Cundliffe, E. Mol. Microbiol. 1994, 13, 349-355. Gandecha, A. R.; Large, S. L.; Cundliffe, E. Gene 1997, 184, 197-203. Fish, S. A.; Cundliffe, E. Microbiology 1997, 143, 3871-3876. Bulter, A. R.; Bate, N.; Cundliffe, E. Chem. Biol. 1999, 6, 287-292).
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Fish, S.A.1
Cundliffe, E.2
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17
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0033133991
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It should be pointed out that the tylIBA and tylLM segments of the tyl cluster have also been sequenced by Cundliffe and co-workers (Merson-Davies, L. A.; Cundliffe, E. Mol. Microbiol. 1994, 13, 349-355. Gandecha, A. R.; Large, S. L.; Cundliffe, E. Gene 1997, 184, 197-203. Fish, S. A.; Cundliffe, E. Microbiology 1997, 143, 3871-3876. Bulter, A. R.; Bate, N.; Cundliffe, E. Chem. Biol. 1999, 6, 287-292).
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Bulter, A.R.1
Bate, N.2
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(a) Thorson, J. S.; Lo, S. F.; Liu, H.-w.; Hutchinson, C. R. J. Am. Chem. Soc. 1993, 115, 5827-5828.
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85069254809
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6tag, the N-terminal amino acid sequencing confirmed that the first 10 residues of TylX3 (AHSSAT-AGPQ) and TylC1 (SGMYVQLGR) are identical to the respective translated tylX3 and tylC1 sequences, except for the deletion of the first methionine residue in both cases.
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-
-
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26
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85069245090
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-
The calculated molecular mass for TylX3 is 55 795 Da, and that for TylC1 is 36 920 Da.
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27
-
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85069242112
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-
2+in TylX3 catalysis must await further investigation.
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28
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0026065201
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Romana, L. K.; Santiago, F. S.; Reeves, P. R. Biochem. Biophys. Res. Commun. 1991, 174, 846-852.
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29
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85069243753
-
-
The incubation mixture was loaded on an Adsorbosphere SAX column (5 μm, 4.6 × 250 mm), and a linear gradient from 140 to 320 mM potassium phosphate buffer (pH 3.6) over 20 min was used to elute the reaction products (monitored at 278 nm). Under these conditions, the retention times were 4.8 min for maltol (6), 13.0 min for substrate 3, and 17.6 min for TDP.
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-
-
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30
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85069251407
-
-
A preparative incubation contained the RfbB product (3, 14.0 mg, 23.7 μmol) and NADPH (21.7 mg, 26.1 μmol) in 1.5 mL of 100 mM potassium phosphate buffer (pH 7.5). The reaction was initiated by the addition of TylX3 (6.5 nmol) and TylC1 (10.5 nmol). Due to the instability of the TylX3 product, an excess of TylC1 was used. The reaction was incubated at room temperature and was followed by monitoring the consumption of NADPH at 340 nm. The reaction was usually complete within 2 h.
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31
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85069239979
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The same HPLC conditions as described in ref 16 were used. The retention time for product 5 was 13.8 min.
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32
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85069250304
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-
2O): δ -10.7 (d, J = 21.9 Hz), -12.9 (d, J = 21.9 Hz).
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