Mechanism of the 2-deoxygenation step in the biosynthesis of the deoxyhexose moieties of the antibiotics granaticin and oleandomycin [8]

Journal of the American Chemical Society

Volumn 121, Issue 11, 1999, Pages 2611-2612

  Draeger, Gerald ^a   Park, Sung Hae ^a   Floss, Heinz G ^a  

^a UNIVERSITY OF WASHINGTON   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ANTIBIOTIC AGENT; GRANATICIN; OLEANDOMYCIN;

CHEMICAL ANALYSIS; CHEMICAL REACTION; DRUG STRUCTURE; DRUG SYNTHESIS; LETTER; NONHUMAN; STRUCTURE ANALYSIS;

EID: 0033599493     PISSN: 00027863     EISSN: None     Source Type: Journal    
DOI: 10.1021/ja9837250     Document Type: Letter

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30. Gra Orf26 and Tü99 Orf11 were quite unstable, losing activity within an hour of cell disruption, but were stabilized somewhat in the presence of PMSF.
31. A P/ACE System 5010 with a P/ACE UV absorbance detector and System Gold software (version 8.1) from Beckman Coulter was used for the CE experiments. The capillary cartidge contained a 57 cm long (50 cm to detector) 75 μm i.d. fused silica capillary. The run buffer was 12.5 mM sodium borate and 16 mM boric acid in demineralized water. The run voltage was 30 kV (normal polarity), and the runs were performed at 25 °C. Samples were injected by pressure (1 s) and detected at 280 nm.
32. Ten μL of a 50x concentrated crude extract of 36 h induced cells (E. coli BL21 DE3 pLysS) containing Gra orf27 was incubated with 0.1 mg of 4 in 40 μL of 10 mM potassium phosphate buffer (pH 7.2) for 30 min at 28 °C. The reaction mixture was heated in boiling water for 2 min, cleared by centrifugation and analyzed by CE.
33. Ten μL each of a 50x concentrated crude extract of 36 h induced cells (E. coli BL21 DE3 pLysS) containing Gra orf27 and Gra orf26 was incubated with 0.1 mg of 4 in 30 μL of 10 mM potassium phosphate buffer (pH 7.2) in the presence of 1 mM NAD(P)H for 30 min at 28 °C. The reaction mixture was heated in boiling water for 2 min, cleared by centrifugation, and analyzed by CE.
34. ret = 4.72 min.
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