An essential role for DNA adenine methylation in bacterial virulence

Science

Volumn 284, Issue 5416, 1999, Pages 967-970

  Heithoff, Douglas M ^a   Sinsheimer, Robert L ^a   Low, David A ^a   Mahan, Michael J ^a  

^a UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

DNA ADENINE METHYLTRANSFERASE; LIVE VACCINE;

ANIMAL EXPERIMENT; ARTICLE; BACTERIAL COLONIZATION; BACTERIAL VIRULENCE; ENZYME ACTIVITY; GENE EXPRESSION REGULATION; GENE INSERTION; METHYLATION; MOUSE; NONHUMAN; PRIORITY JOURNAL; SALMONELLA TYPHIMURIUM; TYPHOID FEVER;

ADENINE; ANIMALS; BACTERIAL PROTEINS; BACTERIAL VACCINES; DNA METHYLATION; DNA, BACTERIAL; GENE EXPRESSION REGULATION, BACTERIAL; GENES, BACTERIAL; LETHAL DOSE 50; METHYLATION; MICE; MICE, INBRED BALB C; MUTATION; PEYER'S PATCHES; SALMONELLA INFECTIONS, ANIMAL; SALMONELLA TYPHIMURIUM; SITE-SPECIFIC DNA-METHYLTRANSFERASE (ADENINE-SPECIFIC); VACCINES, ATTENUATED; VIRULENCE;

ANIMALIA; BACTERIA (MICROORGANISMS); MURINAE; NEGIBACTERIA; SALMONELLA TYPHIMURIUM; TYPHIMURIUM;

EID: 0033532378     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.284.5416.967     Document Type: Article

References (31)

1. M. G. Marinus, in Escherichia coli and Salmonella: Cellular and Molecular Biology, F. Neidhardt, Ed. (American Society for Microbiology, Washington, DC, ed. 2, 1996), pp. 782-791.
2. J. A. Roberts et al., J. Urol. 133, 1068 (1985).
3. M. van der Woude, B. Braaten, D. Low, Trends Microbiol. 4, 5 (1996).
4. The dam102::Mud-Cm and mutS121::Tn10 alleles (and additional alleles below) were transduced into virulent S. typhimurium strain 14028, resulting in strains MT2116 and MT2127, respectively. damΔ232 (MT2188) was constructed using internal oligonucleotides that serve as polymerase chain reaction primers designed to construct an in-frame 300-bp deletion of defined dam sequence. dcm1::Km (MT2198) was constructed according to (26); the Km resistance determinant is associated with an internal deletion of >600 bp of dcm sequence. lrp31::Km is a null insertion in the lrp gene (MT2126).
5. J. E. LeClerc, B. Li, W. L. Payne, T. A. Cebula, Science 274, 1208 (1996).
6. E. B. Newman, R. T. Lin, R. D'Ari, in (1), pp. 1513-1525.
7. B. A. Braaten, X. Nou, L. S. Kaltenbach, D. A. Low, Cell 76, 577 (1994).
8. D. M. Heithoff et al., Proc. Natl. Acad. Sci. U.S.A. 94, 934 (1997).
9. C. P. Conner, D. M. Heithoff, S. M. Julio, R. L. Sinsheimer, M. J. Mahan, ibid. 95, 4641 (1998).
10. M. J. Mahan, J. M. Slauch, J. J. Mekalanos, Science 259, 666 (1993).
11. M. J. Mahan et al., Proc. Natl. Acad. Sci. U.S.A. 92, 669 (1995).
12. P. A. Gulig et al., Mol. Microbiol. 7, 825 (1993).
13. K. L. Roland, L. E. Martin, C. R. Esther, J. Spitznagel. J. Bacteriol. 75, 4154 (1993).
14. E. Garcia Vescovi, F. C. Soncini, E. A. Groisman, Cell 84, 165 (1996).
15. C. F. Earhart, in (1), pp. 1075-1090.
16. E. A. Groisman and F. Heffron, in Two-Component Signal Transductlon, J. A. Hoch and T. J. Silhavy, Eds. (American Society for Microbiology, Washington, DC, 1995), pp. 319-332.
17. D. M. Heithoff and M. J. Mahan, unpublished data.
18. M. van der Woude, W. B. Hale, D. A. Low, J. Bacteriol. 180, 5913 (1998).
19. W. B. Hale, M. W. van der Woude, D. A. Low, ibid. 176, 3438 (1994).
20. S. Tavazoie and C. M. Church, Nature Biotechnol. 16, 566 (1998).
21. R. Bandyopadhyay and J. Das, Gene 140, 67 (1994).
22. Sanger Centre Web site (www.sanger.ac.uk).
23. F. R. Blattner et al., Science 277, 1453 (1997).
24. R. D. Fieischmann et al., ibid. 269, 496 (1995).
25. C. M. Fraser et al., ibid. 281, 375 (1998).
26. S. M. Julio, C. P. Conner, D. M. Heithoff, M. J. Mahan, Mol. Gen. Genet. 258, 178 (1998).
27. D. M. Heithoff et al., J. Bacteriol. 181, 799 (1999).
28. J. M. Slauch and T. Silhavy, ibid. 173, 4039 (1991).
29. C. L. Smith and C. R. Cantor, Methods Enzymol. 155, 449 (1987).
30. M. G. Marinus, A. Poteete, J. A. Arraj, Gene 28, 123 (1984).
31. We thank J. Roth for the dam102::Mud-Cm allele, T. Cebula for the mutS121::Tn10 allele, R. Ballester for critically reading the manuscript, and D. Hillyard for constructing the lrp31 mutant. Supported by NIH grant Al36373 and a Beckman Young Investigator Award (M.J.M.) and NIH grant Al23348 (D.A.L.).