High mutation frequencies among Escherichia coli and Salmonella pathogens

Science

Volumn 274, Issue 5290, 1996, Pages 1208-1211

  LeClerc, J Eugene ^a   Li, Baoguang ^a   Payne, William L ^a   Cebula, Thomas A ^a  

^a CENTER FOR DRUG EVALUATION AND RESEARCH   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ALLELE; ANTIBIOTIC RESISTANCE; ARTICLE; BACTERIAL GENE; BACTERIAL VIRULENCE; BACTERIUM MUTANT; ESCHERICHIA COLI; GENE MUTATION; PHENOTYPE; PRIORITY JOURNAL; SALMONELLA ENTERICA;

ADENOSINE TRIPHOSPHATASES; BACTERIAL PROTEINS; CLONING, MOLECULAR; DISEASE OUTBREAKS; DNA REPAIR; DNA-BINDING PROTEINS; DRUG RESISTANCE, MICROBIAL; ESCHERICHIA COLI; ESCHERICHIA COLI INFECTIONS; ESCHERICHIA COLI O157; ESCHERICHIA COLI PROTEINS; EVOLUTION; FOOD MICROBIOLOGY; GENETIC COMPLEMENTATION TEST; HUMANS; MOLECULAR SEQUENCE DATA; MUTATION; MUTS DNA MISMATCH-BINDING PROTEIN; PHENOTYPE; POLYMERASE CHAIN REACTION; RECOMBINATION, GENETIC; SALMONELLA; SALMONELLA FOOD POISONING; SELECTION (GENETICS); SEQUENCE DELETION; SIGMA FACTOR; VIRULENCE;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; PROKARYOTA; SALMONELLA; SALMONELLA ENTERICA;

EID: 0029860724     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.274.5290.1208     Document Type: Article

References (41)

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19. -4 or less.
20. -4 or less.
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36. R, 1.3 ± 0.7.
37. Strains were transformed with multicopy plasmid clones harboring the wild-type gene for mutH (pGW1899), mutL (pGW1842), mutS (pGW1811), and uvrD (pGT26), pGW plasmids are described by P. P. Pang, A. S. Lundberg, and G. C. Walker [J. Bacteriol. 163, 1007 (1985)], and pGT26 plasmids are described by G. Taucher-Scholz and H. Hoffmann-Berling [Eur. J. Biochem. 137, 573 (1983)]. Mutators C396 and SL78 were transformed by phage P22 HT int transducing particles carrying plasmid clones [M. J. Orbach and E. N. Jackson, J. Bacterial 149, 985 (1982)]. Other mutators were transformed by electroporation with the use of a Bio-Rad Gene Pulser apparatus and protocol supplied by the manufacturer.
38. Strains were transformed with multicopy plasmid clones harboring the wild-type gene for mutH (pGW1899), mutL (pGW1842), mutS (pGW1811), and uvrD (pGT26), pGW plasmids are described by P. P. Pang, A. S. Lundberg, and G. C. Walker [J. Bacteriol. 163, 1007 (1985)], and pGT26 plasmids are described by G. Taucher-Scholz and H. Hoffmann-Berling [Eur. J. Biochem. 137, 573 (1983)]. Mutators C396 and SL78 were transformed by phage P22 HT int transducing particles carrying plasmid clones [M. J. Orbach and E. N. Jackson, J. Bacterial 149, 985 (1982)]. Other mutators were transformed by electroporation with the use of a Bio-Rad Gene Pulser apparatus and protocol supplied by the manufacturer.
39. Strains were transformed with multicopy plasmid clones harboring the wild-type gene for mutH (pGW1899), mutL (pGW1842), mutS (pGW1811), and uvrD (pGT26), pGW plasmids are described by P. P. Pang, A. S. Lundberg, and G. C. Walker [J. Bacteriol. 163, 1007 (1985)], and pGT26 plasmids are described by G. Taucher-Scholz and H. Hoffmann-Berling [Eur. J. Biochem. 137, 573 (1983)]. Mutators C396 and SL78 were transformed by phage P22 HT int transducing particles carrying plasmid clones [M. J. Orbach and E. N. Jackson, J. Bacterial 149, 985 (1982)]. Other mutators were transformed by electroporation with the use of a Bio-Rad Gene Pulser apparatus and protocol supplied by the manufacturer.
40. The primers used were R3, 5′-TTACCTGAGTGCCTACGCC; F2, 5′-CTGGCGGATAAAAGCTCCC; S8, 5′-GCCCATGATGCAGCAGTAT; and L119, 5′-TGCATCTCGATGCACTGGAG Long PCR was done with the Perkin Elmer XL PCR kit and 30 cycles of 1 min at 94°C, 1.5 min at 55°C, and 5 min at 68°C.
41. The authors thank M. J. Bessman, H. Ochman, R. M. Schaaper, P.I. Tarr, and T. S. Whittam for supplying bacterial strains and E. F. Boyd, E. C. Cox, P. E. Hartman, R. E. Lenski, P. Modrich, and P. D. Sniegowski for helpful discussions and comments.