Directed formation of chromosomal deletions in Salmonella typhimurium: Targeting of specific genes induced during infection

Molecular and General Genetics

Volumn 258, Issue 1-2, 1998, Pages 178-181

  Julio, S M ^a   Conner, C P ^a   Heithoff, D M ^a   Mahan, M J ^a  

^a UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

Deletion; In vivo expression technology (IVET); Mutagenesis

Indexed keywords

ARTICLE; BACTERIAL GENE; CHROMOSOME DELETION; GENE TARGETING; MARKER GENE; MUTAGENESIS; NONHUMAN; PRIORITY JOURNAL; SALMONELLA TYPHIMURIUM; SALMONELLOSIS;

CHROMOSOME DELETION; GENE EXPRESSION REGULATION; MUTAGENESIS; SALMONELLA TYPHIMURIUM; VIRULENCE;

ANIMALIA; BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; SALMONELLA; SALMONELLA TYPHIMURIUM; TYPHIMURIUM;

EID: 0031899620     PISSN: 00268925     EISSN: None     Source Type: Journal    
DOI: 10.1007/s004380050721     Document Type: Article

References (22)

1. Black W, Falkow S (1987) Construction and characterization of Bordetella pertussis toxin mutants. Infect Immun 55:2465-2470
2. Chan RK, Botstein D, Watanabe T, Ogata Y (1972) Specialized transduction of tetracycline resistance by phage P22 in Salmonella typhimurium. II. Properties of a high-frequency-transducing lysate. Virology 50:883-898
3. Conner CP, Heithoff DM, Julio SM, Sinsheimer RL, Mahan MJ (1998) Differential patterns of acquired virulence genes distinguish Salmonelia strains. Proc Natl Acad Sci USA. in press
4. Davis RW, Botstein D, Roth JR (1980) Advanced bacterial genetics. Cold Spring Harbor Laboratory Press. Cold Spring Harbor, New York
5. Demerec M, Aldelberg E, Clark AJ, Hartman P (1966) A proposal for a uniform nomenclature in bacterial genetics. Genetics 54:61-76
6. Donnenberg MS, Kaper JB (1991) Construction of an eae deletion mutant of enteropathogenic Escherichia coli by using a positive-selection suicide vector. Infect Immun 59:4310-4317
7. Falkow S (1996) The evolution of pathogenicity in Escherichia, Shigella, and Salmonella. In: Neidhardt FC, Curtiss R III, Ingraham JL, Lin ECC, Low KB, Magasanik B, Reznikoff WS, Riley M, Schaechter M, Umbarger HE (eds) Escherichia coli and Salmonella: cellular and molecular biology (2nd edn). American Society for Microbiology. Washington DC, pp 2723-2729
8. Gay P, Le Coq D, Steinmetz M, Berkelman T, Cado CI (1985) Positive selection procedure for entrapment of insertion sequence elements in gram-negative bacteria. J Bacteriol 164:918-921
9. Gillen J, Willis D, Clark A (1981) Genetic analysis of the RecE pathway of genetic recombination in Escherichia coli K-12. J Bacteriol 145:521-32
10. Groisman EA, Ochman H (1996) Pathogenicity islands: bacterial evolution in quantum leaps. Cell 87:791-794
11. Hacker J, Blum-Oehler G, Muhldorfer I, Tschape H (1997) Pathogenicity islands of virulent bacteria: structure, function, and impact on molecular evolution. Mol Microbiol 23:1089-1097
12. Heithoff DM, Conner CP, Hanna PC, Julio SM, Hentschel U, Mahan MJ (1997a) Bacterial infection as assessed by vivo gene expression. Proc Natl Acad Sci USA 94:934-939
13. Heithoff DM, Conner CP, Mahan MJ (1997b) Dissecting the biology of a pathogen during infection. Trends Microbiol, Ash, C. (ed), J. Elesvier Trends 5:509-513
14. Hughes KT, Roth JR (1985) Directed formation of deletions and duplications using Mud (Ap, lac). Genetics 109:263-282
15. Mahan MJ, Roth JR (1991) Ability of bacterial chromosome segment to invert is dictated by included material rather than flanking sequence. Genetics 129:1021-1032
16. Mahan MJ, Slauch JM, Mekalanos JJ (1993a) Selection of bacterial virulence genes that are specifically induced in host tissues. Science 259:666-668
17. Mahan MJ, Slauch JM, Mekalanos JJ (1993b) Bacteriophage P22 transduction of integrated plasmids: single-step cloning of Salmonella typhimurium gene fusions. J Bacteriol 175:7086-7091
18. Mahan MJ, Tobias JW, Slauch JM, Hanna PC, Collier JR, Mekalanos JJ (1995) Antibiotic-based selection for bacterial genes that are specifically induced during infection of a host. Proc Natl Acad Sci USA 92:669-673
19. Maniatis T, Fritsch E, Sambrook J (1982) Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York
20. Rainey P, Heithoff DM, Mahan MJ (1997) Single-step conjugative cloning of bacterial fusions involved in microbial-host interactions. Mol Gen Genet 256:84-87
21. Schmieger H (1972) Phage P22 mutants with increased or decreased transduclion abilities. Mol Gen Genet 119:75-88
22. Wang R, Kushner S (1990) Construction of versatile low-copy-number vectors for cloning, sequencing, and gene expression in Escherichia coli. Gene 100:195-99