Study of ligand-receptor interactions by fluorescence correlation spectroscopy with different fluorophores: Evidence that the homopentameric 5- hydroxytryptamine type 3(As) receptor binds only one ligand

Biochemistry

Volumn 38, Issue 27, 1999, Pages 8671-8681

  Wohland, T ^a   Friedrich, K ^a   Hovius, R ^a   Vogel, H ^a  

^a EPFL   (Switzerland)

Author keywords

[No Author keywords available]

Indexed keywords

1,2,3,9 TETRAHYDRO 3 [(5 METHYL 1H IMIDAZOL 4 YL)METHYL] 9 (3 AMINOPROPYL) 4H CARBAZOL 4 ONE; LIGAND; RADIOLIGAND; SEROTONIN 3 ANTAGONIST; SEROTONIN 3 RECEPTOR; UNCLASSIFIED DRUG;

ARTICLE; ELECTROMAGNETIC FIELD; FLUORESCENCE; LIGAND BINDING; PRIORITY JOURNAL; RECEPTOR BINDING; RECEPTOR INTRINSIC ACTIVITY; SIGNAL NOISE RATIO; SPECTROSCOPY; STOICHIOMETRY;

4-CHLORO-7-NITROBENZOFURAZAN; ANIMALS; BINDING SITES; CALIBRATION; CARBAZOLES; CARBOCYANINES; CELL LINE; CRICETINAE; FLUORESCENT DYES; GRANISETRON; IMIDAZOLES; LIGANDS; MODELS, CHEMICAL; RADIOLIGAND ASSAY; RECEPTORS, SEROTONIN; RECEPTORS, SEROTONIN, 5-HT3; RHODAMINES; SEROTONIN ANTAGONISTS; SPECTROMETRY, FLUORESCENCE;

EID: 0033529238     PISSN: 00062960     EISSN: None     Source Type: Journal    
DOI: 10.1021/bi990366s     Document Type: Article

References (43)

1. Ehrenberg, M., and Rigler, R. (1974) Chem. Phys. 4, 390-401.
2. Ehrenberg, M., and Rigler, R. (1976) Q. Rev. Biophys. 9, 69-81.
3. Elson, E. L., and Madge, D. (1974) Biopolym. 13, 1-27.
4. Madge, D., Elson, E. L., and Webb, W. W. (1974) Biopolymers 13, 1-27.
5. Eigen, M., and Rigler, R. (1994) Proc. Natl. Acad. Sci. U.S.A. 91, 5740-5747.
6. Edelstein, J., Schaad, O., and Changeux, J.-P. (1997) Biochemistry 55, 13755-13760.
7. Rigler, R., Widengren, J., and Mets, Ü. (1992) in Fluorescence spectroscopy (Wolfbeis, O. S., Ed.) pp 13-24, Springer-Verlag, Berlin, Heidelberg, and New York.
8. Thompson, N. L. (1991) in Topics in fluorescence spectroscopy, Volume 1: Techniques (Lakowicz, J. R., Ed.) pp 337-378, Plenum Press, New York.
9. Widengren, J. (1996) Fluorescence Correlation Spectroscopy, Photophysical Aspects and Applications, Ph.D. Thesis, Department of Medical Biophysics, Karolinska Institute, Stockholm, Sweden.
10. Haupts, U., Maiti, S., Schwille, P., and Webb, W. W. (1998) Proc. Natl. Acad. Sci. U.S.A. 95, 13573-13578.
11. Rauer, B., Neumann, E., Widengren, J., and Rigler, R. (1996) Biophys. Chem. 58, 3-12.
12. Klingler, J., and Friedrich, T. (1997) Biophys. J. 73, 2195-2200.
13. Knowles, J. (1997) Curr. Opin. Biotechnol. 8, 667-668.
14. Hamon, M. (1992) in Neuroscience Perspectives (Jenner, P., Ed.) Academic Press Ltd., London.
15. Schmid, E. L., Tairi, A.-P., Hovius, R., and Vogel, H. (1998) Anal. Chem. 70, 1331-1338.
16. Tairi, A.-P., Hovius, R., Pick, H., Blasey, H., Bernard, A., Surprenant, A., Lundström, K., and Vogel, H. (1998) Biochemistry 37, 15850-15864.
17. Unwin, N. (1993) Cell/Neuron 72, 31-41.
18. Edelstein, S. J., and Bardsley, W. G. (1997) J. Mol. Biol. 267, 10-16.
19. Davies, A., Pistis, M., Hanna, M., Peters, J., Lambert, J., Hales, T., and Kirkness, E. (1999) Nature 397, 359-363.
20. Aragon, S. R., and Pecora, R. (1976) J. Chem. Phys. 64, 1791-1803.
21. Rigler, R., Mets, U., Widengren, J., and Kask, P. (1993) Eur. Biophys. J. 22, 169-175.
22. Widengren, J., Rigler, R., and Mets, Ü. (1994) J. Fluoresc. 4, 255-258.
23. Widengren, J., Mets, Ü., and Rigler, R. (1995) J. Phys. Chem. 99, 13368-13379.
24. Chandrasekhar, S. (1943) Rev. Mod. Phys. 15, 1-89.
25. Clegg, R. M., and Vaz, W. L. C. (1985) in Progress in Protein-Lipid Interactions (Watts, A., and DePont, J. J. H. H. M., Eds.) pp 173-229, Elsvier Science, Amsterdam.
26. Lundström, K., Michel, A., Blasey, H., Bernard, A. R., Hovius, R., Vogel, H., and Surprenant, A. (1997) J. Recept. Signal Transduct. Res. 17, 115-126.
27. Hovius, R., Tairi, A.-P., Blasey, H., Bernard, A., Lundström, K., and Vogel, H. (1998) J. Neurochem. 70, 824-834.
28. Saleh, B. E. A., and Teich, M. C. (1991) Fundamentals of Photonics, John Wiley & Sons, Inc., New York.
29. Koppel, D. E. (1974) Phys. Rev. A 10, 1938-1945.
30. Meseth, U., Wohland, T., and Vogel, H. (1999) Biophys. J. 76, 1619-1631.
31. Cheng, Y. C., and Prusoff, W. H. (1973) Biochem. Pharmacol. 22, 3099-3108.
32. Song, L., Varma, C. A., Verhoeven, J. W., and Tanke, H. J. (1996) Biophys. J. 70, 2959-2968.
33. Bhairi, S. M. (1997) Detergents, Calbiochem-Novabiochem Corporation, La Jolla, CA.
34. Tanford, C., Nozaki, Y., and Rohde, M. F. (1977) J. Phys. Chem. 81, 1555-1560.
35. Gordon, J. C., Sarbin, N. S., Barefoot, D. S., and Pinkus, L. M. (1990) Eur. J. Pharmacol. 188, 313-319.
36. McKernan, R. M., Biggs, C. S., Gillard, N., Quirk, K., and Ragan, C. I. (1990) Biochem. J. 269, 623-628.
37. Boess, F. G., Beroukhim, R., and Martin, I. L. (1995) J. Neurochem. 64, 1401-1405.
38. Unwin, N. (1993) J. Mol. Biol. 229, 1101-1124.
39. Elias, H. G. (1984) Macromolecules, 2nd ed., Plenum Press, New York.
40. Palma, E., Bertrand, S., Binzoni, T., and Bertrand, D. (1996) J. Physiol. 491, 151-161.
41. Walter, N. G., Schwille, P., and Eigen, M. (1996) Proc. Natl. Acad. Sci. U.S.A. 93, 12805-12810.
42. Bonnet, G., Krichevsky, O., and Libchaber, A. (1998) Proc. Natl. Acad. Sci. U.S.A. 95, 8602-8606.
43. Kinjo, M., Nishimura, G., Koyoma, T., Mets, Ü., and Rigler, R. (1998) Anal. Biochem. 260, 166-172.