Site-specific interaction of thrombin and inhibitors observed by fluorescence correlation spectroscopy

Biophysical Journal

Volumn 73, Issue 4, 1997, Pages 2195-2200

  Klingler, Jürgen ^a   Friedrich, Thomas ^a  

^a BASF SE   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

THROMBIN; THROMBIN INHIBITOR;

ARTICLE; BINDING SITE; ENZYME ACTIVE SITE; FLUORESCENCE SPECTROSCOPY; LIGAND BINDING; PROTEIN PROTEIN INTERACTION;

ACARI;

EID: 0030866750     PISSN: 00063495     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0006-3495(97)78251-1     Document Type: Article

References (18)

1. Berne, B. J., and R. Pecora. 1976. Dynamic Light Scattering. Wiley, New York.
2. Böhm, H. J., G. Klebe, and H. Kubiny. 1996. Wirkstoffdesign. Spektrum Akademischer Verlag, Heidelberg.
3. Campbell, P., editor. 1996. Intelligent drug design. Nature. 384(Suppl. to issue no. 6604, 7 November 1996).
4. Davie, E. W., K. Fujikawa, and W. Kisiel. 1991. The coagulation cascade: initiation, maintainance and regulation. Biochemistry. 30:10363-10370.
5. Dodt, J. 1995. Gerinnungshemmende Wirkstoffe blutsaugender Tiere: vom Hirudin zu Hirudinmimetica. Angew. Chem. 107:948-962.
6. Friedrich, T., B. Kröger, S. Bialojan, H.-G. Lemaire, H. W. Höffken, P. Reuschenbach, M. Otte, and J. Dodt. 1993. A kazal-type inhibitor with thrombin specifity from Rhodnius prolixus. J. Biol. Chem. 268: 16216-16222.
7. Jackson, C. M. 1980. Blood coagulation. Annu. Rev. Biochem. 49: 765-811.
8. Magde, D., E. Elson, and W. W. Webb. 1972. Thermodynamic fluctuations in a reacting system - measurement by fluorescence correlation spectroscopy. Phys. Rev. Lett. 29:705-708.
9. Markwardt, F. 1995. Untersuchungen über Hirudin. Naturwissenschaft. 42:537-538.
10. Rigler, R. 1995. Fluorescence correlations, single molecule detection and large number screening: applications in biotechnology. J. Biotechnol. 41:177-186.
11. Rigler, R., Ü. Mets, J. Widengren, and P. Kask. 1993a. Fluorescence correlation spectroscopy with high count rate and low background: analysis of tranlational diffusion. Eur. Biophys. J. 22:169-175.
12. Rigler, R., J. Widengren, and Ü. Mets. 1993b. Interactions and kinetics of single molecules as observed by fluorescence correlation spectroscopy. In Fluorescence Spectroscopy. O. Wolfbeis, editor. Springer Verlag, Berlin.
13. Stubbs, M. T., and W. Bode. 1993. Crytal structures of thrombin and thrombin complexes as a framework for antithrombotic drug design. Perspect. Drug Discovery Design. 1:431-452
14. Thompson, N. L. 1991. Fluorescence Correlation Spectroscopy. In Topics in Fluorescence Spectroscopy, Vol. 1: Techniques. J. R. Lakowicz, editor. Plenum, New York
15. van de Locht, A., D. Lambda, M. Bauer, R. Huber, T. Friedrich, B. Kröger, W. Höffken, and W. Bode. 1995. Two heads are better than one: crystal structure of the insect derived double domain kazal inhibitor rhodniin in complex with thrombin. EMBO J. 14:5149-5157.
16. van de Locht, A., M. T. Stubbs, W. Bode, T. Friedrich, C. Bollschweiler, W. Höffken, and R. Huber. 1996. The ornithodorin: thrombin crystal structure, a model to decipher the TAP enigma. EMBO J. 15: 6011-6017.
17. Widengren, J., R. Rigler, and Ü. Mets. 1994. Triplet-state monitoring by fluorescence correlation spectroscopy. J. Fluorescence. 4:255-258.
18. Wu, Q., M. Tsiang, and J. E. Sadler. 1992. Localization of the single-stranded DNA-binding site in the thrombin anion-binding exosite. J. Biol. Chem. 267:24408-24412.