Organogenic role of B lymphocytes in mucosal immunity

Science

Volumn 286, Issue 5446, 1999, Pages 1965-1968

  Golovkina, Tatyana V ^a   Shlomchik, Mark ^a   Hannum, Lynn ^a   Chervonsky, Alexander ^a  

^a NONE

Author keywords

[No Author keywords available]

Indexed keywords

ANIMAL CELL; ANIMAL MODEL; ANTIBODY PRODUCTION; ANTIGEN PRESENTATION; ARTICLE; B LYMPHOCYTE; IMMUNE SYSTEM; INTESTINE LYMPHATIC TISSUE; MOUSE; MUCOSAL IMMUNITY; NONHUMAN; PEYER PATCH; PRIORITY JOURNAL; VIRUS INFECTION;

ANIMALS; B-LYMPHOCYTES; CELL COUNT; GENES, IMMUNOGLOBULIN; IMMUNITY, MUCOSAL; IMMUNOGLOBULIN M; LYMPHOCYTE ACTIVATION; MAMMARY TUMOR VIRUS, MOUSE; MICE; MICE, INBRED STRAINS; MICE, KNOCKOUT; MICE, TRANSGENIC; MICROSCOPY, ELECTRON, SCANNING; PEYER'S PATCHES; RETROVIRIDAE INFECTIONS; T-LYMPHOCYTES; TUMOR VIRUS INFECTIONS;

EID: 0033521087     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.286.5446.1965     Document Type: Article

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43. PP generation is a multistep process in which primary "anlage" development precedes population with lymphocytes [S. Adachi, H. Yoshida, H. Kataoka, S.-I. Nishikawa, Int. Immunol. 9, 507 (1997); H. Yoshida et al., Int. Immunol. 11, 643 (1999)].
44. PP generation is a multistep process in which primary "anlage" development precedes population with lymphocytes [S. Adachi, H. Yoshida, H. Kataoka, S.-I. Nishikawa, Int. Immunol. 9, 507 (1997); H. Yoshida et al., Int. Immunol. 11, 643 (1999)].
45. These PP formation centers are too small to be detected using a stereomicroscope; however, the appearance of 9 ± 0.6 well-developed PPs in adult Igh-6 mice 14 days after B6 BM transfer supports the idea that the anlagen are predeveloped by a B cell-independent mechanism.
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47. L. E. Malick and R. B. Wilson, Stain Technol. 50, 265 (1975); J. Nation, Stain Technnol. 58, 347 (1983); L. E. Malick and R. B. Wilson, in Scanning Electron Microscopy, O. Johari, Ed. (IIT Research Institute, Chicago, 1975), p. 256. Briefly, intestinal fragments containing PP were cleaned from mucus and fixed in 2.5% glutaraldehyde in 0.1% cacodylate buffer overnight at 4°C. Specimens were treated with alternating 1% osmium tetroxide and 1% thiocarbohydrazide (OTOTO method), dehydrated in graded series of acetone, critical point-dried, and sputter-coated to produce 15-nm gold coating. Samples were examined using a Jeol 35 scanning electron microscope at an accelerating voltage of 15 kV.
48. L. E. Malick and R. B. Wilson, Stain Technol. 50, 265 (1975); J. Nation, Stain Technnol. 58, 347 (1983); L. E. Malick and R. B. Wilson, in Scanning Electron Microscopy, O. Johari, Ed. (IIT Research Institute, Chicago, 1975), p. 256. Briefly, intestinal fragments containing PP were cleaned from mucus and fixed in 2.5% glutaraldehyde in 0.1% cacodylate buffer overnight at 4°C. Specimens were treated with alternating 1% osmium tetroxide and 1% thiocarbohydrazide (OTOTO method), dehydrated in graded series of acetone, critical point-dried, and sputter-coated to produce 15-nm gold coating. Samples were examined using a Jeol 35 scanning electron microscope at an accelerating voltage of 15 kV.
49. We thank L. Bechtold for her excellent assistance with SEM, M. Neutra for help with SEM data interpretation, and P. Cherry for secretarial assistance. Supported in part by NIH grants CA65795 (T.V.G.), DK53561 (A.C.), CA34196 (T.V.G. and A.C.), and AI43603 (M.S.); the Merck Foundation (A.C.); and Jackson Laboratory grants (A.C. and T.V.G.).