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1842300633
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7 cells/ml).
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1842292326
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note
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125I-protein A (Amersham). The radioactivity in the filters was counted in a Packard counter. The nonspecific binding was quantified by incubation with 125I-protein A alone. Protein immunoblots were done as described in (27).
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13
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0030023173
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18
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1842368642
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note
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About 106 follicle cells in 100 μI were injected in the duodenal mucosa, between the pylorus and the first visible PP. The injection location was marked by a surgical thread. The injected duodenal segments were resected after 9 days, washed with phosphate-buffered saline, and either embedded in Tissue-Tek (Miles, Elkhart, IN) and snap frozen for immunocytochemistry or fixed in 2% glutaraldheyde in 0.1 M cacodylate buffer at pH 7.4 for light and electron microscopy.
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21
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1842291686
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note
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10 beads/ml), placed in the apical compartment of the filter, incubated at 4°C, and switched at 37°C. Aliquots of the medium from the basolateral compartment were taken at different times. The bead numbers are given as arbitrary units that represent the number of fluorescent events per volume unit quantified with a FACScan flow cytometer. Each point represents the average value of three separate experiments.
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23
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0029164871
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J. Mestecky, Ed. Plenum, New York
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T. C. Savidge and M. W. Smith, in Advances in Mucosal Immunology, J. Mestecky, Ed. (Plenum, New York, 1995), pp. 239-241.
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26
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1842375817
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note
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8 bacteria) and incubated for 1 hour at 4° and 37°C. Transepithelial resistance was measured by a Millicell Ohmmeter (Millipore, Saint Quentin Yvelines, France) before and after the infection. Intracellular bacteria were counted after incubation of the filters with gentamycin (50 μg/ml). Epithelial cells were than lysed and the suspension was plated on colonization factor agar (CFA). We counted translocated bacteria by plating the medium from the basolateral compartment on CFA. Results are given as the number of CFU per square centimeter of confluent cells.
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27
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0022181709
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H. P. Hauri, E. E. Sterchi, D. Bienz, J. A. Fransen, A. Marxer, J. Cell Biol. 101, 838 (1985).
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Detolla, L.J.4
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30
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1842403723
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note
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+-ATPase the polymeric Ig receptor, respectively. Supported by the Swiss National Science Foundation, Sandoz, Zyma, Muschamp, the Ipsen and Singer Polignac Foundations, the Pasteur Institute, and INSERM.
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