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Volumn 277, Issue 5328, 1997, Pages 949-952

Conversion by Peyer's patch lymphocytes of human enterocytes into M cells that transport bacteria

Author keywords

[No Author keywords available]

Indexed keywords

ARTICLE; BACTERIAL TRANSLOCATION; CONTROLLED STUDY; HUMAN; HUMAN CELL; INTESTINE CELL; LYMPH FOLLICLE; MONOLAYER CULTURE; PEYER PATCH; PRIORITY JOURNAL; VIBRIO CHOLERAE;

EID: 0038946003     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.277.5328.949     Document Type: Article
Times cited : (556)

References (30)
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    • R. Förster et al., Cell 87, 1037 (1996).
    • (1996) Cell , vol.87 , pp. 1037
    • Förster, R.1
  • 8
    • 1842300633 scopus 로고    scopus 로고
    • note
    • 7 cells/ml).
  • 10
    • 0026773096 scopus 로고
    • E. Friederich et al., Cell 70, 81 (1992).
    • (1992) Cell , vol.70 , pp. 81
    • Friederich, E.1
  • 12
    • 1842292326 scopus 로고    scopus 로고
    • note
    • 125I-protein A (Amersham). The radioactivity in the filters was counted in a Packard counter. The nonspecific binding was quantified by incubation with 125I-protein A alone. Protein immunoblots were done as described in (27).
  • 18
    • 1842368642 scopus 로고    scopus 로고
    • note
    • About 106 follicle cells in 100 μI were injected in the duodenal mucosa, between the pylorus and the first visible PP. The injection location was marked by a surgical thread. The injected duodenal segments were resected after 9 days, washed with phosphate-buffered saline, and either embedded in Tissue-Tek (Miles, Elkhart, IN) and snap frozen for immunocytochemistry or fixed in 2% glutaraldheyde in 0.1 M cacodylate buffer at pH 7.4 for light and electron microscopy.
  • 21
    • 1842291686 scopus 로고    scopus 로고
    • note
    • 10 beads/ml), placed in the apical compartment of the filter, incubated at 4°C, and switched at 37°C. Aliquots of the medium from the basolateral compartment were taken at different times. The bead numbers are given as arbitrary units that represent the number of fluorescent events per volume unit quantified with a FACScan flow cytometer. Each point represents the average value of three separate experiments.
  • 26
    • 1842375817 scopus 로고    scopus 로고
    • note
    • 8 bacteria) and incubated for 1 hour at 4° and 37°C. Transepithelial resistance was measured by a Millicell Ohmmeter (Millipore, Saint Quentin Yvelines, France) before and after the infection. Intracellular bacteria were counted after incubation of the filters with gentamycin (50 μg/ml). Epithelial cells were than lysed and the suspension was plated on colonization factor agar (CFA). We counted translocated bacteria by plating the medium from the basolateral compartment on CFA. Results are given as the number of CFU per square centimeter of confluent cells.
  • 30
    • 1842403723 scopus 로고    scopus 로고
    • note
    • +-ATPase the polymeric Ig receptor, respectively. Supported by the Swiss National Science Foundation, Sandoz, Zyma, Muschamp, the Ipsen and Singer Polignac Foundations, the Pasteur Institute, and INSERM.


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.