Repressor-AFLR interaction modulates aflatoxin biosynthesis in Aspergillus parasiticus

Mycopathologia

Volumn 147, Issue 2, 1999, Pages 105-112

  Chang, Perng Kuang ^a   Yu, Jiujiang ^a   Bhatnagar, Deepak ^a   Cleveland, Thomas E ^a  

^a SOUTHERN REGIONAL RESEARCH CENTER   (United States)

Author keywords

Aflatoxin; aflR; Aspergillus parasiticus; Repressor; Sclerotia

Indexed keywords

AFLATOXIN; AFLR PROTEIN, ASPERGILLUS; DNA BINDING PROTEIN; FUNGAL PROTEIN; NITRITE; NITRITE REDUCTASE; NITROGEN; PRIMER DNA; QUATERNARY AMMONIUM DERIVATIVE; REPRESSOR PROTEIN; TRANSCRIPTION FACTOR;

ARTICLE; ASPERGILLUS; BIOSYNTHESIS; GENETIC TRANSFORMATION; GENETICS; GROWTH, DEVELOPMENT AND AGING; METABOLISM;

AFLATOXINS; ASPERGILLUS; DNA PRIMERS; DNA-BINDING PROTEINS; FUNGAL PROTEINS; NITRITE REDUCTASES; NITRITES; NITROGEN; QUATERNARY AMMONIUM COMPOUNDS; REPRESSOR PROTEINS; TRANSCRIPTION FACTORS; TRANSFORMATION, GENETIC;

ASPERGILLUS PARASITICUS; SOLANUM TUBEROSUM;

EID: 0033340484     PISSN: 0301486X     EISSN: None     Source Type: Journal    
DOI: 10.1023/A:1007157309168     Document Type: Article

References (28)

1. Bhatnagar D, Cleveland TE, Brown RL, Cary JW, Yu J, Chang P-K. Preharvest aflatoxin contamination: elimination through biotechnology. In: Dhaliwal GS, Randhawa NS, Arora R, Dhawan AK, eds. Ecological Agriculture and Sustainable Development. Vol. 1, Indian Ecological Society and Center for Research in Rural & Industrial Development. Chaman Enterprises, New Delhi. 1998: 110-129.
2. Payne GA, Brown MP. Genetics and physiology of aflatoxin biosynthesis. Annu Rev Phytopathol 1998; 36: 329-362.
3. Yu J, Chang P-K, Cary JW, Wright M, Bhatnagar D, Cleveland TE, Payne GA, Linz JE. Comparative mapping of aflatoxin pathway gene clusters in Aspergillus parasiticus and Aspergillus flavus. Appl Environ Microbiol 1995; 61: 2365-2371.
4. Brown DW, Yu JH, Kelkar HS, Fernandes M, Nesbitt TC, Keller NP, Adams TH, Leonard TJ. Twenty-five coregulated transcripts define a sterigmatocystin gene cluster in Aspergillus nidulans. Proc Natl Acad Sci USA 1996; 93: 1418-1422.
5. Chang P-K, Ehrlich KC, Yu J, Bhatnagar D, Cleveland TE. Increased expression of Aspergillus parasiticus aflR, encoding a sequence-specific DNA-binding protein, relieves nitrate inhibition of aflatoxin biosynthesis. Appl Environ Microbiol 1995; 61: 2372-2377.
6. Woloshuk CP, Foutz KR, Brewer JW, Bhatnagar D, Cleveland TE, Payne GA. Molecular characterization of aflR, a regulatory locus for aflatoxin biosynthesis. Appl Environ Microbiol 1994; 60: 2408-2414.
7. Yu J-H, Butchko RAE, Fernandes M, Keller NP, Leonard TJ, Adams TH. Conservation of structure and function of the aflatoxin regulatory gene aflR from Aspergillus nidulans and A. flavus. Curr Genet 1996; 29: 549-555.
8. Ehrlich KC, Montalbano BG, Cary JW. Binding of the C6-zinc cluster protein, AFLR, to the promoters of aflatoxin pathway biosynthesis genes in Aspergillus parasiticus. Gene 1999; 260: 249-257.
9. Fernandes M, Keller NP, Adams TH. Sequence-specific binding by Aspergillus nidulans AFLR, a C6 zinc cluster protein regulating mycotoxin biosynthesis. Mol Microbiol 1998; 28: 1355-1365.
10. Chang P-K, Yu J, Bhatnagar D, Cleveland TE. The carboxy-terminal portion ofthe aflatoxin pathway regulatory protein AFLR of Aspergillus parasiticus activates GAL1::lacZ gene expression in Saccharomyces cerevisiae. Appl Environ Microbiol 1999; 65: 2508-2512.
11. Hicks JK, Yu J-H, Keller NP, Adams TH. Aspergillus sporulation and mycotoxin production both require inactivation of the FadA Gα protein-dependent signaling pathway. EMBO J 1997; 16: 4916-4923.
12. Meyers DM, Bhatnagar D, Payne GA. Characterization of aflJ, a gene involved in aflatoxin biosynthesis. Appl Environ Microbiol 1998; 64: 3713-3717.
13. Flaherty JE, Payne GA. Overexpression of AFLR leads to upregulation of pathway gene transcription and increased aflatoxin production in Aspergillus flavus. Appl Environ Microbiol 1997; 63: 3995-4000.
14. Liu B-H, Chu FS. Regulation of aflR and its product, AflR, associated with aflatoxin biosynthesis. Appl Environ Microbiol 1998; 64: 3718-3723.
15. Ehrlich KC, Montalbano BG, Bhatnagar D, Cleveland TE. Alteration of different domains in AFLR affects aflatoxin pathway metabolism in Aspergillus parasiticus transformants. Fungal Genet Bio 1998; 23: 279-287.
16. Ma J, Ptashne M. The carboxy-terminal 30 amino acids of GAL4 are recognized by GAL80. Cell 1987; 50: 137-142.
17. Lamb HK, Newton GH, Levett L, Cairns E, Roberts CF, Hawkins AR. The QUTA activator and QUTR repressor proteins of Aspergillus nidulans interact to regulate transcription of the quinate utilization pathway gene. Microbiology 1996; 142: 1477-1490.
18. Xiao X, Fu Y-H, Marzluf GA. The negative-acting NMR regulatory protein of Neurospora crassa binds to and inhibits the DNA-binding activity of the positive-acting nitrogen regulatory protein NIT2. Biochemistry 1995; 34: 8861-8868.
19. 2. Appl Environ Microbiol 1998; 64: 4834-4841.
20. Cove DJ. The induction and repression of nitrate reductase in the fungus Aspergillus nidulans. Biochim Biophys Acta 1966; 113: 51-56.
21. Horng JS, Chang P-K, Pestka JJ, Linz JE. Development of a homologous transformation system for Aspergillus parasiticus with the gene encoding nitrate reductase. Mol Gen Genet 1990; 224: 294-296.
22. Chang P-K, Ehrlich KC, Linz JE, Bhatnagar D, Cleveland TE, Bennett JW. Characterization of the Aspergillus parasiticus niaD and niiA gene cluster. Curr Genet 1996; 30: 68-75.
23. Adye J, Mateles RI. Incorporation of labeled compounds into aflatoxins. Biochim Biophys Acta 1964; 86: 418-420.
24. Trnovsky J. Semi-dry electroblotting of DNA and RNA from agarose and polyacrylamide gels. Biotechniques 1992; 13: 800-804.
25. Wu TS, Linz JE. Cloning and functional analysis of a β-tubulin gene from a benomyl resistant mutant of Aspergillus parasiticus. Gene 1996; 182: 7-12.
26. Cleveland TE, Bhatnagar D, Brown RL. Aflatoxin production via cross-feeding of pathway precursors during cofermentation of aflatoxin pathway-blocked Aspergillus parasiticus mutants. Appl Environ Microbiol 1991; 57: 2907-2911.
27. Todd, RB, Andrianopoulos A. Evolution of a fungal regulatory gene family: the Zn(II)2Cys6 binuclear cluster DNA binding motif. Fungal Genet Biol 1997; 21: 388-405.
28. Chang P-K, Cary JW, Bhatnagar D, Cleveland TE, Bennett JW, Linz JE, Woloshuk CP, Payne GA. Cloning of the Aspergillus parasiticus apa-2 gene associated with the regulation of aflatoxin biosynthesis. Appl Environ Microbiol 1993; 59: 3273-3279.