Aspergillus sporulation and mycotoxin production both require inactivation of the FadA Gα protein-dependent signaling pathway

EMBO Journal

Volumn 16, Issue 16, 1997, Pages 4916-4923

  Hicks, Julie K ^a   Yu, Jae Hyuk ^a   Keller, Nancy P ^a   Adams, Thomas H ^a  

^a TEXAS A AND M UNIVERSITY   (United States)

Author keywords

Aflatoxin; Fungal development; G protein; RGS domain; Secondary metabolism

Indexed keywords

FUNGAL PROTEIN; GUANINE NUCLEOTIDE BINDING PROTEIN; MYCOTOXIN; PROTEIN SUBUNIT; STERIGMATOCYSTIN; TRANSCRIPTION FACTOR;

ARTICLE; ASPERGILLUS NIDULANS; CONTROLLED STUDY; FUNGAL GENETICS; FUNGUS MUTANT; GENE EXPRESSION REGULATION; NONHUMAN; PRIORITY JOURNAL; SIGNAL TRANSDUCTION; SPOROGENESIS;

AFLATOXINS; ASPERGILLUS; ASPERGILLUS NIDULANS; CHROMATOGRAPHY, THIN LAYER; FUNGAL PROTEINS; GENE EXPRESSION REGULATION, FUNGAL; GENES, FUNGAL; GTP-BINDING PROTEINS; GTPASE-ACTIVATING PROTEINS; MUTATION; PROTEINS; RNA, MESSENGER; SIGNAL TRANSDUCTION; SPORES, FUNGAL; STERIGMATOCYSTIN; TRANSCRIPTION, GENETIC;

ASPERGILLUS; EMERICELLA NIDULANS; FUNGI;

EID: 0030839254     PISSN: 02614189     EISSN: None     Source Type: Journal    
DOI: 10.1093/emboj/16.16.4916     Document Type: Article

References (46)

1. Abdollahi, A. and Buchanan, R.L. (1981) Regulation of aflatoxin biosynthesis: induction of aflatoxin production by various carbohydrates. J. Food Sci., 46, 633-635.
2. Adams, T.H. and Timberlake, W.E. (1990) Developmental repression of growth and gene expression in Aspergillus. Proc. Natl Acad. Sci. USA, 87, 5405-5409.
3. Adams, T.H., Boylan, M.T. and Timberlake, W.E. (1988) brlA is necessary and sufficient to direct conidiophore development in Aspergillus nidulans. Cell, 54, 353-362.
4. 1. J. S.Afr. Chem. Inst., 23, 170-177.
5. Beppu, T. (1992) Secondary metabolites as chemical signals for cellular differentiation. Gene, 115, 159-165.
6. Berman, D.M., Wilkie, T.M. and Gilman, A.G. (1996) GAIP and RGS4 are GTPase activating proteins (GAPs) for the Gi subfamily of G protein α subunits. Cell, 86, 445-452.
7. Berse, B., Dmochowska, A., Skrzypek, M., Weglenski, P., Bates, M.A. and Weiss, R.L. (1983) Cloning and characterization of the ornithine carbamoyltransferase gene from Aspergillus nidulans. Gene, 25, 109-117.
8. Betina, V. (1995) Differentiation and secondary metabolism in some prokaryotes and fungi. Folia Microbiol., 40, 51-67.
9. Brown, D.W., Yu, J.-H., Kelkar, H.S., Fernandes, M., Nesbitt, T.C., Keller, N.P., Adams, T.H. and Leonard, T.J. (1996) Twenty-five coregulated transcripts define a sterigmatocystin gene cluster in Aspergillus nidulans. Proc. Natl Acad. Sci. USA, 93, 1418-1422.
10. Chater, K.F., Bruton, C.J., Plaskitt, K.A., Buttner, M.J., Mendez, C. and Helmann, J.D. (1989) The developmental fate of S. coelicolor hyphae depends upon a gene product homologous with the motility σ factor of B. subtilis. Cell, 59, 133-143.
11. Choi, G.H., Chen, B. and Nuss, D.L. (1995) Virus-mediated or transgenic suppression of a G-protein α subunit and attenuation of fungal virulence. Proc. Natl Acad. Sci. USA, 92, 305-309.
12. Cole, R.J. and Cox, R.H. (1981) In Cole, R.J. and Cox, R.H (eds), Handbook of Toxic Fungal Metabolites. Academic Press, New York, NY, pp. 67-93.
13. De Vries, L., Mousli, M., Wurmser, A. and Farquhar, M.G. (1995) GAIP, a protein that specifically interacts with the trimeric G protein Gαi3, is a member of a protein family with a highly conserved core domain. Proc. Natl Acad. Sci. USA, 92, 11916-11920.
14. Demain, A.L. (1992) Microbial secondary metabolism: a new theoretical frontier for academia, a new opportunity for industry. Ciba Foundn Symp., 171, 3-16.
15. Dietzel, C. and Kurjan, J. (1987) Pheromonal regulation and sequence of the Saccharomyces cerevisiae SST2 gene: a model for desensitization to pheromone. Mol. Cell. Biol., 7, 4169-4177.
16. Dohlman, H.G. and Thorner, J. (1997) RGS proteins and signaling by heterotrimeric G proteins. J. Biol. Chem., 272, 3871-3874
17. Dohlman, H.G., Apaniesk, D., Chen, Y., Song, J. and Nusskern, D. (1995) Inhibition of G-protein signaling by dominant gain-of-function mutations in Sst2p, a pheromone desensitization factor in Saccharomyces cerevisiae. Mol. Cell. Biol., 15, 3635-3643.
18. Dohlman, H.G., Song, J., Ma, D., Courchesne, W.E. and Thorner, J. (1996) Sst2, a negative regulator of pheromone signaling in the yeast Saccharomyces cerevisiae: expression, localization, genetic interaction and physical association with Gpa1 (G protein α subunit). Mol. Cell. Biol., 16, 5194-5209.
19. Druey, K.M., Blumer, K.J., Kang, V.H. and Kehrl, J.H. (1996) Inhibition of G-protein-mediated MAP kinase activation by a new mammalian gene family. Nature, 379, 742-746.
20. Han, S., Navarro, J., Greve, R.A. and Adams, T.H. (1993) Translational repression of brlA expression prevents premature development in Aspergillus. EMBO J., 12, 2449-2457.
21. Hopwood, D.A. (1988) Towards and understanding of gene switching in Streptomyces, the basis of sporulation and antibiotic production. Proc. R. Soc. Lond. B, 235, 121-138.
22. Horinouchi, S. (1996) Streptomyces genes involved in aerial mycelium formation. FEMS Microbiol. Lett., 141, 1-9.
23. Janzen, D.H. (1977) Why fruits rot, seeds mold and meat spoils. Am. Naturalist, 111, 691-713.
24. Käfer, E. (1977) Meiotic and mitotic recombination in Aspergillus and its chromosomal aberrations. Adv. Genet., 19, 33-131.
25. Kale, S.P., Bhatnagar, D. and Bennett, J.W. (1994) Isolation and characterization of morphological variants of Aspergillus parasiticus deficient in secondary metabolite production. Mycol. Res., 98, 645-652.
26. Kale, S.P., Cary, J.W., Bhatnagar, D. and Bennet, J.W. (1996) Characterization of experimentally induced, nonaflatoxigenic variant strains of Aspergillus parasiticus. Appl. Environ. Microbiol., 62, 3399-3404.
27. Keller, N.P., Kantz, N.J. and Adams, T.H. (1994) Aspergillus nidulans verA is required for production of the mycotoxin sterigmatocystin. Appl. Environ. Microbiol., 60, 1444-1450.
28. Koelle, M.R. and Horvitz, H.R. (1996) EGL-10 regulates G protein signaling in the C. elegans nervous system and shares a conserved domain with many mammalian proteins. Cell, 84, 115-125.
29. Lee, B.N. and Adams, T.H. (1994a) The Aspergillus nidulans fluG gene is required for production of an extracellular developmental signal. Genes Dev., 8, 641-651.
30. Lee, B.N. and Adams, T.H. (1994b) Overexpression of flbA, an early regulator of Aspergillus asexual sporulation leads to activation of brlA and premature initiation of development. Mol. Microbiol., 14, 323-334.
31. Lee, B.N. and Adams, T.H. (1996) fluG and flbA function interdependently to initiate conidiophore development in Aspergillus nidulans through brlAβ activation. EMBO J., 15, 299-309.
32. Pontecorvo, G., Roper, J.A., Hemmons, L.M., MacDonald, K.D. and Bufton, A.W.J. (1953) The genetics of Aspergillus nidulans. Adv. Genet., 5, 141-238.
33. Rens-Domiano, S. and Hamm, H.E. (1995) Structural and functional relationships of heterotrimeric G-proteins. FASEB J., 9, 1059-1066.
34. Stack, M. and Rodricks, J.V. (1971) Method for analysis and chemical confirmation of sterigmatocystin. J. Ass. Official Anal. Chemists, 54, 86-90.
35. Trail, F., Chang, P.K., Cary, J. and Linz, J.E. (1994) Structural and functional analysis of the nor-1 gene involved in the biosynthesis of aflatoxins by Aspergillus parasiticus. Appl. Environ. Microbiol., 60, 4078-4085.
36. Turner, G.E. and Borkovich, K.A. (1993) Identification of a G protein α subunit from Neurospora crassa that is a member of the Gi family. J. Biol. Chem., 268, 14805-14811.
37. Watson, N., Linder, M.E., Druey, K.M., Kehrl, J.H. and Blumer, K.J. (1996) RGS family members: GTPase-activating proteins for heterotrimeric G-protein α-subunits. Nature, 383, 172-175.
38. Wicklow, D.T. (1990) Adaptations in Aspergillus flavus. Trans. Mycol. Soc. Japan, 31, 511-23.
39. Wicklow, D.T., Dowd, P.F. and Gloer, J.B. (1994) In Powell, K.A., Renwick, A. and Peberdy, J.F. (eds), Genus Aspergillus: from Taxonomy and Genetics to Industrial Application. Plenum Press, New York, pp. 93-112.
40. Wieser, J. and Adams, T.H. (1995) flbD encodes a myb-like DNA binding protein that controls initiation of Aspergillus nidulans conidiophore development. Genes Dev., 9, 491-502.
41. Wieser, J., Lee, B.N., Fondon, J.W. and Adams, T.H. (1994) Genetic requirements for initiating asexual development in Aspergillus nidulans. Curr. Genet., 27, 62-69.
42. Woloshuk, C.P., Foutz, K.R., Brewer, J.F., Bhatnagar, D., Cleveland, T.E. and Payne, G.A. (1994) Molecular characterization of aflR, a regulatory locus for aflatoxin biosynthesis. Appl. Environ. Microbiol., 60, 2408-2414.
43. Yelton, M.M., Hamer, J.E. and Timberlake, W.E. (1984) Transformation of Aspergillus nidulans by using a trpC plasmid. Proc. Natl Acad. Sci. USA, 81, 1470-1474.
44. Yu, J.-H. and Leonard, T.J. (1995) Sterigmatocystin biosynthesis in Aspergillus nidulans requires a novel type I polyketide synthase. J. Bacteriol., 177, 4792-4800.
45. Yu, J.-H., Butchko, R.A.E., Fernandes, M., Keller, N.P., Leonard, T.J. and Adams, T.H. (1996a) Conservation of structure and function of the aflatoxin regulatory gene aflR from Aspergillus nidulans and A. flavus. Curr. Genet., 29, 549-555.
46. Yu, J.-H., Wieser, J. and Adams, T.H. (1996b) The Aspergillus FlbA RGS domain protein antagonizes G-protein signaling to block proliferation and allow development. EMBO J., 15, 5184-5190.