Binding of the C6-zinc cluster protein, AFLR, to the promoters of aflatoxin pathway biosynthesis genes in Aspergillus parasiticus

Gene

Volumn 230, Issue 2, 1999, Pages 249-257

  Ehrlich, K C ^a   Montalbano, B G ^a   Cary, J W ^a  

^a SOUTHERN REGIONAL RESEARCH CENTER   (United States)

Author keywords

Electrophoretic mobility shift assay; Fungi; Gene expression; Secondary metabolism

Indexed keywords

AFLATOXIN; DNA BINDING PROTEIN;

ARTICLE; ASPERGILLUS PARASITICUS; BINDING SITE; ELECTROPHORETIC MOBILITY; GENE CLUSTER; GENE EXPRESSION; GENE SEQUENCE; NONHUMAN; PRIORITY JOURNAL; PROMOTER REGION; PROTEIN BINDING;

AFLATOXINS; ASPERGILLUS; BINDING SITES; DNA FOOTPRINTING; DNA-BINDING PROTEINS; FUNGAL PROTEINS; GENE EXPRESSION REGULATION, FUNGAL; GENES, FUNGAL; NUCLEAR PROTEINS; PROMOTER REGIONS (GENETICS); RECOMBINANT PROTEINS; TRANSCRIPTION FACTORS;

EID: 0033574753     PISSN: 03781119     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0378-1119(99)00075-X     Document Type: Article

References (27)

1. Andrianopoulos A., Hynes M.J. Sequence and functional analysis of the regulatory gene, amdR from Aspergillus nidulans. Mol. Cell. Biol. 10:1990;3194-3203.
2. Bhatnagar D., Cleveland T.E., Cotty P.J. Mycological aspects of aflatoxin formation. Eaton D.L., Groopman J.D. The Toxicology of Aflatoxins: Human Health, Veterinary, and Agricultural Significance. 1994;Academic Press, San Diego, CA.
3. Buchanan R.L., Jones S.B., Gerasimowicz W.V., Zaika L.L., Stahl H.G. Regulation of aflatoxin biosynthesis: assessment of the role of cellular energy status as a regulator of the induction of aflatoxin production. Appl. Environ. Microbiol. 53:1987;1224-1231.
4. Burger G., Strauss J., Scazzocchio C., Lang B. nirA, the pathway specific regulatory gene of nitrate assimilation in Aspergillus nidulans encodes a putative GAL4-type zinc finger protein and contains introns in highly conserved regions. Mol. Cell. Biol. 11:1991;5746-5755.
5. Carey M., Kakidani J., Leatherwood J., Mostashari F., Ptashne M. An amino-terminal fragment of GAL4 binds DNA as a dimer. J. Mol. Biol. 209:1989;423-432.
6. Chang P.K., Cary J.W., Bhatnagar D., Cleveland T.E., Bennett J.W., Linz J.E., Woloshuk C.P., Payne G.A. Cloning of the Aspergillus parasiticus apa-2 gene associated with the regulation of aflatoxin biosynthesis. Appl. Environ. Microbiol. 59:1993;3273-3279.
7. Chang P.K., Ehrlich K.C., Yu J., Bhatnagar D., Cleveland T.E. Increased expression of Aspergillus parasiticus aflR, encoding a sequence-specific DNA-binding protein, relieves nitrate inhibition of aflatoxin biosynthesis. Appl. Environ. Microbiol. 61:1995;2372-2377.
8. Ehrlich K.C., Montalbano B.G. Binding of an aflatoxin biosynthesis regulatory protein to the 5′-upstream promoter region of several aflatoxin pathway genes. FASEB J. 9:1995;A940.
9. Ehrlich K.C., Montalbano B.G., Bhatnagar D., Cleveland T.E. Alteration of different domains in AFLR affects aflatoxin pathway metabolism in Aspergillus parasiticus transformants. Fungal Genet. Biol. 23:1998;279-287.
10. Ehrlich K.C., Montalbano B.G., Cary J.W. Characterization of the promoter for the gene encoding the aflatoxin biosynthetic pathway regulatory protein AFLR. Biochim. Biophys. Acta. 1444:1999;412-417.
11. Evans R.M., Hollenberg S.M. Zinc fingers: gilt by association. Cell. 52:1988;1-3.
12. Fernandes M., Keller N.P., Adams T.H. Sequence-specific binding by Aspergillus nidulans AFLR, a C6 zinc cluster protein regulating mycotoxin biosynthesis. Mol. Microbiol. 28:1998;1355-1365.
13. Galas D.J., Schmitz A. DNase footprinting: a simple method for detection of protein-DNA binding specificity. Nucleic Acids Res. 5:1978;3157-3170.
14. Garabedian M.J., Labaer J., Liu W.H., Thomas J.R. Analysis of protein-DNA interactions. Hames B.D., Higgins S.J. Gene Transcription: A Practical Approach. 1993;IRL Press, Oxford.
15. Hellauer K., Rochon M.H., Turcotte B. A novel DNA binding motif for yeast zinc cluster proteins: the Leu3p and Pdr3p transcriptional activators recognize everted repeats. Mol. Cell. Biol. 16:1996;6096-6102.
16. Lamb H.K., Hawkins A.R., Smith M., Harvey I.J., Brown J., Turner G., Roberts C.F. Spatial and biological characterization of the complete quinic acid gene cluster in Aspergillus nidulans. Mol. Gen. Genet. 223:1990;17-23.
17. Lundin M., Nehlin J.O., Ronne H. Importance of a flanking AT-rich region in target site recognition by the GC-box-binding zinc finger protein MIG1. Mol. Cell. Biol. 14:1994;1979-1985.
18. Marmorstein R., Carey M., Ptashne M., Harrison S.C. DNA recognition by GAL4: structure of a protein-DNA complex. Nature. 356:1992;408-414.
19. Payne G.A., Nystorm G.J., Bhatnagar D., Cleveland T.E., Woloshuk C.P. Cloning of the afl-2 gene involved in aflatoxin biosynthesis from Aspergillus flavus. Appl. Environ. Microbiol. 59:1993;156-162.
20. Reece R.J., Ptashne M. Determinants of binding-site specificity among yeast C6 zinc cluster proteins. Science. 261:1993;909-911.
21. Todd R.B., Andrianopoulos A. Evolution of a fungal regulatory gene family: the Zn(II)2Cys6 binuclear cluster DNA binding motif. Fungal Genet. Biol. 21:1997;388-405.
22. Todd R.B., Andrianopoulos A., Davis M.A., Hynes M.J. FacB, the Aspergillus nidulans activator of acetate utilization genes, binds dissimilar DNA sequences. EMBO J. 17:1998;2042-2054.
23. Trail F., Mahanti N., Rarick M., Mehigh R., Liang S.H., Zhou R., Linz J.E. A physical and transcriptional map of the aflatoxin gene cluster in Aspergillus parasiticus and the functional disruption of a gene involved early in the aflatoxin pathway. Appl. Environ. Microbiol. 61:1995;2665-2673.
24. Vashee S., Xu H., Johnston S.A., Kodadek T. How do 'Zn2Cys6' proteins distinguish between similar upstream activation sites? J. Biol. Chem. 268:1993;24699-24706.
25. Woloshuk C.P., Foutz K.R., Brewer J.F., Bhatnagar D., Cleveland T.E., Payne G.A. Molecular characterization of aflR, a regulatory locus for aflatoxin biosynthesis. Appl. Environ. Microbiol. 60:1994;2408-2414.
26. Yu J.H., Butchko R.A., Fernandes M., Keller N.P., Leonard T.J., Adams T.H. Conservation of structure and function of the aflatoxin regulatory gene aflR from Aspergillus nidulans and A. flavus. Curr. Genet. 29:1996;549-555.
27. Yu J.J., Chang P.K., Cary J.W., Wright M., Bhatnagar D., Cleveland T.E., Payne G.A., Linz J.E. Comparative mapping of aflatoxin pathway gene clusters in Aspergillus parasiticus and Aspergillus flavus. Appl. Environ. Microbiol. 61:1995;2365-2371.