Creation of a stable human reporter cell line suitable for FACS-based, transdominant genetic selection

Somatic Cell and Molecular Genetics

Volumn 25, Issue 4, 1999, Pages 191-205

  Richards, B ^a   Karpilow, J ^a   Dunn, C ^a   Zharkikh, L ^a   Maxfield, A ^a   Kamb, A ^a   Teng, D H F ^a  

^a Arcaris Incorporated   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

RETINOIC ACID;

ARTICLE; CELL LINE; CELL SELECTION; DOSE RESPONSE; GENE INDUCTION; GENETIC SELECTION; GENETIC TRANSCRIPTION; GENETIC TRANSDUCTION; HUMAN; HUMAN CELL; MELANOMA CELL; PRIORITY JOURNAL; REPORTER GENE;

BASE SEQUENCE; CELL SEPARATION; DNA; DNA PRIMERS; FLOW CYTOMETRY; GENES, DOMINANT; GENES, REPORTER; GREEN FLUORESCENT PROTEINS; HUMANS; LUMINESCENT PROTEINS; MELANOMA; RETROVIRIDAE; SELECTION (GENETICS); TRANSDUCTION, GENETIC; TUMOR CELLS, CULTURED;

EID: 0033277185     PISSN: 07407750     EISSN: None     Source Type: Journal    
DOI: 10.1023/A:1019206625658     Document Type: Article

References (37)

1. Forward genetics in mammalian cells: Functional approaches to gene discovery
2. Chemical genetics: Ligand-based discovery of gene function
3. Transdominant genetics, peptide inhibitors, and drug targets
4. Transdominant genetic analysis of a growth control pathway
5. "Mutagenesis" by peptide aptamers identifies genetic network members and pathway connections
6. Genetic selection of peptide inhibitors of biological pathways
7. Cloning mammalian genes by expression selection of genetic suppressor elements: Association of kinesin with drug resistance and cell immortalization
8. Isolation of peptide aptamers that inhibit intracellular processes
9. A combination of genetic suppressor elements produces resistance to drugs inhibiting DNA replication
10. Synthetic promoter elements obtained by nucleotide sequence variation and selection for activity
11. Detecting lacZ gene expression in living cells with new lipophilic, fluorogenic beta-galactosidase substrates
12. Quantitation of transcription and clonal selection of single living cells with beta-lactamase as reporter
13. Synthetic muscle promoters: Activities exceeding naturally occurring regulatory sequences
14. Assays for transcriptional activity based on the luciferase reporter gene
15. Selection and analysis of a mutant cell line defective in the hypoxia-inducible factor-1 alpha-subunit (HIF-1alpha). Characterization of hif-1alpha-dependent and -independent hypoxia-inducible gene expression
16. Signal-regulated activation of serum response factor is mediated by changes in actin dynamics
17. Applications of green fluorescent protein as a marker of retroviral vectors
18. Flow cytometric analysis and FACS sorting of cells based on GFP accumulation
19. Identification of a retinoic acid responsive element in the retinoic acid receptor beta gene
20. A retinoic acid receptor-specific element controls the retinoic acid receptor-beta promoter
21. Retinoid-dependent in vitro transcription mediated by the RXR/RAR heterodimer
22. Myeloid differentiation and retinoblastoma phosphorylation changes in HL-60 cells induced by retinoic acid receptor- and retinoid X receptor-selective retinoic acid analogs
23. Activation of retinoid X receptors induces apoptosis in HL-60 cell lines
24. Vesicular stomatitis virus G glycoprotein pseudotyped retroviral vectors: Concentration to very high titer and efficient gene transfer into mammalian and nonmammalian cells
25. Green fluorescent protein as a scaffold for intracellular presentation of peptides
26. Functional analysis of a retroviral host-range mutant: Altered long terminal repeat sequences allow expression in embryonal carcinoma cells
27. Embryonic stem cell virus, a recombinant murine retrovirus with expression in embryonic stem cells
28. A genome-wide functional assay of signal transduction in living mammalian cells
29. Single cell assay of a transcription factor reveals a threshold in transcription activated by signals emanating from the T-cell antigen receptor
30. Measurement of ligand-induced activation in single viable T cells using the lacZ reporter gene
31. The biochemical basis of an all-or-none cell fate switch in Xenopus oocytes
32. Effects of novel RAR- and RXR-selective retinoids on myeloid leukemic proliferation and differentiation in vitro
33. Retinoic acid induces apoptosis-associated neural differentiation of a murine teratocarcinoma cell line
34. The era of pathway quantification
35. Attenuation of green fluorescent protein half-life in mammalian cells
36. Generation of destabilized green fluorescent protein as a transcription reporter
37. A novel method to isolate cells with conditional gene expression using fluorescence activated cell sorting