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A mammalian gene with introns instead of exons generating stable RNA products
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Classification of gas5 as a multi-small-nucleolar-RNA (snoRNA) host gene and a member of the 5′-terminal oligopyrimidlne gene family reveals common features of snoRNA host genes
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Smith CM, Steitz JA: Classification of gas5 as a multi-small-nucleolar-aRNA (snoRNA) host gene and a member of the 5′-terminal oligopyrimidlne gene family reveals common features of snoRNA host genes. Mol Cell Biol 1998, 18:6897-6909. This reports the second multi-snoRNA host gene. Like UHG, the gas5 spliced RNA becomes polyadenylated and associates with translating ribosomes but does not encode a functional polypeptide. 5′-TOP sequences are shown to be present in all vertebrate snoRNA host genes.
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Smith, C.M.1
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The host gene for intronic U17 small nucleolar RNAs in mammals has no protein-coding potential and is a member of the 5′-terminal oligopyrimidine gene family
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Pelczar P, Filipowicz W: The host gene for intronic U17 small nucleolar RNAs in mammals has no protein-coding potential and is a member of the 5′-terminal oligopyrimidine gene family. Mol Cell Biol 1998, 18:4509-4518. U17HG contains a two variants of a single box H/ACA snoRNA. Its transcript is enriched in the cytoplasm but it does not associate with translating ribosomes. It does have a 5′-TOP sequence like UHG [43] and gas5 [44••].
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Pelczar, P.1
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46
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Human U19 intron-encoded snoRNA is processed from a long primary transcript that possesses little potential for protein coding
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Bortolin M-L, Kiss T: Human U19 intron-encoded snoRNA is processed from a long primary transcript that possesses little potential for protein coding. RNA 1998, 4:445-454. U19HG contains a box H/ACA snoRNA and has a 5′ sequence reminiscent of 5′-TOP messages. Unlike UHG [43], gas5 [44••] and U17HG [45•], the U19HG spliced RNA remains in the nucleus.
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Bortolin, M.-L.1
Kiss, T.2
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Conserved boxes C and D are essential nucleolar localization elements of U14 and U8 snoRNAs
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Lange TS, Borovjagin A, Maxwell ES, Gerbi SA: Conserved boxes C and D are essential nucleolar localization elements of U14 and U8 snoRNAs. EMBO J 1998, 17:3176-3187.
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Lange, T.S.1
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Inhibition of protein dephosphorylation results in the accumulation of splicing snRNPs and coiled bodies within the nucleolus
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Lyon CE, Bohmann K, Sleeman J, Lamond AI: Inhibition of protein dephosphorylation results in the accumulation of splicing snRNPs and coiled bodies within the nucleolus. Exp Cell Res 1997, 230:84-93. This reports the observation that okadaic acid (a general serine/threonine phosphatase inhibitor) treatment of HeLa cells can cause coiled bodies to appear within the nucleolus. The effect is reversible and supports a functional link between the two subnuclear regions.
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A box H/ACA small nucleolar RNA-like domain at the human telomerase 3′ end
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Mitchell JR, Cheng J, Collins K: A box H/ACA small nucleolar RNA-like domain at the human telomerase 3′ end. Mol Cell Biol 1999, 19:567-576.
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Yu Y-T, Shu M-D, Steitz JA: Modifications of U2 snRNA are required for snRnp assembly and pre-mRNA splicing. EMBO J 1998, 17:5783-5795. U2 snRNA requires 2′-O-methyl groups and pseudouridines at its 5′-end in order to assemble the functional 17S U2 snRNP. Thus, U2 provides a unique opportunity to examine the contributions of individual modifications to a documented function, which is currently impossible with U6 snRNA or rRNA; however, it is not known whether modification of U2 is RNA guided.
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Noon KE, Bruenger E, McCloskey JA: Post-transcriptional modifications in 16S and 23S rRNAs of the archaeal hyperthermophile Sulfolobus solfataricus. J Bacteriol 1998, 180:2883-2888. Analysis of rRNA from S. solfataricus by mass spectrometry revealed an increase in the number of nucleotide modifications as a function of growth temperature. This observation supports a model in which modifications contribute to rRNA stability. The mechanism employed to increase the number or extent of modification(s) is unknown.
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J Bacteriol
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Noon, K.E.1
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The Nop60B gene of Drosophila encodes an essential nucleolar protein that functions in yeast
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Phillips B, Billin AN, Cadwell C, Buchholz R, Erickson C, Merriam JR, Carbon J, Poole SJ: The Nop60B gene of Drosophila encodes an essential nucleolar protein that functions in yeast. Mol Gen Genet 1998,260:20-29.
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Phillips, B.1
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Merriam, J.R.6
Carbon, J.7
Poole, S.J.8
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