-
2
-
-
0026718508
-
-
J. W. B. Hershey, Annu. Rev. Biochem. 60, 717 (1991); W. C. Merrick, Microbiol. Rev. 56, 291 (1992).
-
(1992)
Microbiol. Rev.
, vol.56
, pp. 291
-
-
Merrick, W.C.1
-
3
-
-
0002523042
-
-
J. W. B. Hershey, M. B. Mathews, N. Sonenberg, Eds. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY
-
W. C. Merrick and J. W. B. Hershey, in Translational Control, J. W. B. Hershey, M. B. Mathews, N. Sonenberg, Eds. (Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, 1996), pp. 31-69.
-
(1996)
Translational Control
, pp. 31-69
-
-
Merrick, W.C.1
Hershey, J.W.B.2
-
7
-
-
0018853455
-
-
K. Struhl and R. W. Davis, J. Mol. Biol. 136, 309 (1980); K. Struhl, Nucleic Acids Res. 13, 8587 (1985).
-
(1980)
J. Mol. Biol.
, vol.136
, pp. 309
-
-
Struhl, K.1
Davis, R.W.2
-
8
-
-
0022422342
-
-
K. Struhl and R. W. Davis, J. Mol. Biol. 136, 309 (1980); K. Struhl, Nucleic Acids Res. 13, 8587 (1985).
-
(1985)
Nucleic Acids Res.
, vol.13
, pp. 8587
-
-
Struhl, K.1
-
9
-
-
0026099337
-
-
D. J. Jamieson, B. Rahe, J. Pringle, J. D. Beggs, Nature 349, 715 (1991).
-
(1991)
Nature
, vol.349
, pp. 715
-
-
Jamieson, D.J.1
Rahe, B.2
Pringle, J.3
Beggs, J.D.4
-
10
-
-
0028872606
-
-
V. Thuillier, S. Stettler, A. Sentenac, P. Thuriaux, M. Werner, EMBO J. 14, 351 (1995).
-
(1995)
EMBO J.
, vol.14
, pp. 351
-
-
Thuillier, V.1
Stettler, S.2
Sentenac, A.3
Thuriaux, P.4
Werner, M.5
-
11
-
-
0026090063
-
-
A 2.9-kb Xho I-Sal I DNA fragment containing the complete DED1 gene (6) was subcloned into pRS315 (CEN/LEU2) to yield pDED1009 and incubated with hydroxylamine [R. S. Sikorski and J. D. Boeke, Methods Enzymol. 194, 302 (1991)]. The mutagenized DNA was amplified and transformed into yeast strain YTC75 [MATa ded1::TRP1 ura3-52 lys2-801 ade2-101 trp1-Δ1 his3-Δ200 leu2-Δ1 pDED1008 (DED1/ CEN/URA3)]. Transformants on leucine-dropout plates were replica-plated onto 5-fluoroorotic acid (5-FOA) plates to identify conditional mutants that failed to grow at either 37° or 15°C. Linkage between the growth phenotype and the mutated ded1 allele was verified by recloning the ded1 insert and repeating the plasmid shuffling. Mutations In the ded1 alleles were identified by DNA sequencing.
-
(1991)
Methods Enzymol.
, vol.194
, pp. 302
-
-
Sikorski, R.S.1
Boeke, J.D.2
-
12
-
-
1842390141
-
-
R.-Y. Chuang and T.-H. Chang, data not shown
-
R.-Y. Chuang and T.-H. Chang, data not shown.
-
-
-
-
21
-
-
0022873934
-
-
I. Hussein and M. J. Leibowitz, Gene 46, 13 (1986); N. Iizuka, L. Najita, A. Franzusoff, P. Sarnow, Mol. Cell. Biol. 14, 7322 (1994).
-
(1986)
Gene
, vol.46
, pp. 13
-
-
Hussein, I.1
Leibowitz, M.J.2
-
22
-
-
0028034493
-
-
I. Hussein and M. J. Leibowitz, Gene 46, 13 (1986); N. Iizuka, L. Najita, A. Franzusoff, P. Sarnow, Mol. Cell. Biol. 14, 7322 (1994).
-
(1994)
Mol. Cell. Biol.
, vol.14
, pp. 7322
-
-
Iizuka, N.1
Najita, L.2
Franzusoff, A.3
Sarnow, P.4
-
23
-
-
0024411890
-
-
P. Leroy, P. Alzari, D. Sassoon, D. Wolgemuth, M. Fellous, Cell 57, 549 (1989).
-
(1989)
Cell
, vol.57
, pp. 549
-
-
Leroy, P.1
Alzari, P.2
Sassoon, D.3
Wolgemuth, D.4
Fellous, M.5
-
24
-
-
0026723509
-
-
K. D. Gulyas and T. F. Donahue, ibid. 69, 1031 (1992); H. Yoon, S. Miller, E. K. Pabich, T. F. Donahue, Genes Dev. 6, 2463 (1992).
-
(1992)
Cell
, vol.69
, pp. 1031
-
-
Gulyas, K.D.1
Donahue, T.F.2
-
25
-
-
0027080295
-
-
K. D. Gulyas and T. F. Donahue, ibid. 69, 1031 (1992); H. Yoon, S. Miller, E. K. Pabich, T. F. Donahue, Genes Dev. 6, 2463 (1992).
-
(1992)
Genes Dev.
, vol.6
, pp. 2463
-
-
Yoon, H.1
Miller, S.2
Pabich, E.K.3
Donahue, T.F.4
-
26
-
-
0027760994
-
-
W. J. Feaver et al., Cell 75, 1379 (1993); J. Q. Svejstrup et al., ibid. 80, 21 (1995).
-
(1993)
Cell
, vol.75
, pp. 1379
-
-
Feaver, W.J.1
-
27
-
-
0028885363
-
-
W. J. Feaver et al., Cell 75, 1379 (1993); J. Q. Svejstrup et al., ibid. 80, 21 (1995).
-
(1995)
Cell
, vol.80
, pp. 21
-
-
Svejstrup, J.Q.1
-
31
-
-
0021822441
-
-
S. B. Balm, D. F. Pietras, D. C. Eustice, F. Sherman, Mol. Cell. Biol. 5, 1839 (1985).
-
(1985)
Mol. Cell. Biol.
, vol.5
, pp. 1839
-
-
Balm, S.B.1
Pietras, D.F.2
Eustice, D.C.3
Sherman, F.4
-
32
-
-
0021369651
-
-
600 unit, collected by filtration, washed extensively in 0.1 M potassium phosphate (pH 6.5), and then fixed in 0.1 M potassium phosphate (pH 6.5) buffer containing 3.7% formaldehyde for 10 min. The formaldehyde-fixed cells were digested with zymolyase 100T (Seikagaku America, Rockville, MD) for 40 min at 37°C and washed three times in a phosphate-buffered saline (PBS) solution containing 1 M sorbitol. Cells were then treated with methanol (6 min) and acetone (30 s) at -20°C and resuspended in PBL [PBS, bovine serum albumin (1 mg/ml), and 100 mM lysine] to which purified normal rabbit IgG (3.5 mg/ml) was added at 1:50 dilution for staining overnight at room temperature. The next day, cells were washed three times in PBL, and the secondary antibody, Texas Red-conjugated goat antibody to rabbit IgG (Jackson Labs), was added at 1:50 dilution and incubated at room temperature for 90 min. Cells were then washed again in PBL and stained with 4′,6-diamino-2-phenylindole (DAPI) (0.5 μg/ml). Cells were applied to slides with an equal volume of Citifluor (Marivac, Halifax, Nova Scotia, Canada) and visualized with a Zeiss Axiophot microscope with Normarski optics. Texas Red and ultraviolet filters were used to detect Texas Red-and DAPI-stained images, respectively.
-
(1984)
J. Cell Biol.
, vol.98
, pp. 922
-
-
Kilmartin, J.V.1
Adams, A.E.2
-
33
-
-
1842393126
-
-
unpublished data
-
T.-H. Chang, unpublished data.
-
-
-
Chang, T.-H.1
-
34
-
-
1842309169
-
-
note
-
We thank K. Arndt, A. Hinnebusch, P. Linder, P. Sarnow, K. Struhl, and D. Wolgemuth for plasmids and strains; Y. Liu and A. Tartakoff for performing the in situ hybridization experiments; M. Leibowitz for advice on yeast translation extracts; K. Arndt, T. Donahue, J. Hershey, A. Hinnebusch, K. Madura, W. Merrick, A. Sachs, N. Sonenberg, and J. Woolford for helpful discussions; and J. Abelson for support. Supported by funds from Ohio State University, NIH grant GM48752, and an American Cancer Society (Ohio Division) grant (T.-H.C.).
-
-
-
|