Dynein is required for spindle assembly in cytoplasmic extracts of Spisula solidissima oocytes

Journal of Cell Science

Volumn 112, Issue 9, 1999, Pages 1291-1302

  Palazzo, Robert E ^a,b   Vaisberg, Eugeni A ^b,c   Weiss, Dieter G ^d   Kuznetsov, Sergei A ^b,d   Steffen, Walter ^b,e  

^a UNIVERSITY OF KANSAS   (United States)

^b MARINE BIOLOGICAL LABORATORY   (United States)

^c UNIVERSITY OF COLORADO   (United States)

^d UNIVERSITY OF ROSTOCK   (Germany)

^e UNIVERSITY OF VIENNA   (Austria)

Author keywords

Centrosome; Dynein; Meiosis; Mitosis; Spisula

Indexed keywords

ANTIBODY; DYNEIN ADENOSINE TRIPHOSPHATASE; MONOCLONAL ANTIBODY; ORTHOVANADIC ACID; POTASSIUM CHLORIDE;

AMINO TERMINAL SEQUENCE; ARTICLE; CELL LYSATE; CENTROSOME; CHROMOSOME; CLAM; CYTOPLASM; GERMINAL VESICLE; MEIOSIS; MICROTUBULE; MITOSIS SPINDLE; NONHUMAN; OOCYTE; PRIORITY JOURNAL;

ANIMALS; ANTIBODIES, MONOCLONAL; ANTIGEN-ANTIBODY REACTIONS; BIVALVIA; CELL EXTRACTS; CYTOPLASM; DYNEIN ATPASE; FEMALE; MITOTIC SPINDLE APPARATUS; OOCYTES; VANADATES;

ASTER; BIVALVIA; SPISULA; SPISULA SOLIDISSIMA;

EID: 0033019403     PISSN: 00219533     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (80)

1. Albertson, D. G. and Thomson, J. N. (1993). Segregation of holocentric chromosomes at meiosis in the nematode, Caenorhabditis elegans. Chromosome Res. 1, 15-26.
2. Allen, R. D. (1953). Fertilization and artificial activation in the egg of the surf-clam, Spisula solidissima. Biol. Bull. 105, 213-239.
3. Allen, R. D., Allen, N. S. and Travis, J. L. (1981). Video-enhanced contrast, differential interference contrast (AVEC-DIC) microscopy: a new method capable of analyzing microtubule-related motility in the reticulopodial network of Allogromia laticollaris. Cell Motil. Cytoskel. 1, 291-302.
4. Allen, R. D., Weiss, D. G., Hayden, J. H., Brown, D. T., Fujiwake, H. and Simpson, M. (1985). Gliding movement of and bidirectional transport along single native microtubules from squid axoplasm: evidence for an active role of microtubules in cytoplasmic transport. J. Cell Biol. 100, 1736-1752.
5. Bajer, A. S. and Mole-Bajer, J. (1982). Asters, poles and transport properties within spindle-like microtubule arrays. Cold Sping Harbor Symp. Quant. Biol. 46, 263-283.
6. Bastmeyer, M., Steffen, W. and Fuge, H. (1986). Immunostaining of spindle components in tipulid spermatocytes using a serum against pericentriolar material. Eur. J. Cell Biol. 42, 305-310.
7. Bonaccorsi, S., Giansanti, M. G. and Gatti, M. (1998). Spindle self-organization and cytokinesis during male meiosis in asterless mutants of Drosophila melanogaster. J. Cell Biol. 142, 751-761.
8. Compton, D. A. (1998). Focusing on spindle poles. J. Cell Sci. 111, 1477-1481.
9. Costello, D. P. and Henley, C. (1971). Meihods for Obtaining and Handling Marine Eggs and Embryos. Second Edition. Marine Biological Laboratory. Woods Hole, MA.
10. de Saint Phalle, B. and Sullivan, W. (1998). Spindle assembly and mitosis without centrosomes in parthenogenetic Sciara embryos. J. Cell Biol. 141, 1383-1391.
11. Dessev, G. and Goldman, R. (1988). Meiotic breakdown of nuclear envelope in oocytes of Spisula solidissima involves phosphorylation and release of nuclear lamin. Deb. Biol. 130, 543-550.
12. Dessev, G., Palazzo, R., Rebhun, L. I. and Goldman, R. (1989). Disassembly of the nuclear envelope of Spisula oocytes in a cell free system. Dev. Biol. 131, 496-504.
13. cdc2 and cyclin B phosphorylates the nuclear lamin and disassembles nuclei of clam oocytes in vitro. J. Cell. Biol. 112, 523-533.
14. Gaglio, T., A., Saredi, Bingham, J. B., Hasbani, M. J., Gill, S. R. Schroer, T. A. and Compton, D. A. (1996). Opposing motor activities are required for the organization of the mammalian mitotic spindle pole. J Cell Biol. 135, 399-414.
15. Gaglio, T., Dionne, M. A. and Compton, D. A. (1997). Mitotic spindle poles are organized by structural and motor proteins in addition to centrosomes. J. Cell Biol. 138, 1055-1066.
16. Gerbi, S. A. (1986). Unusual Chromosome movements in Sciarid flies. In: Results and Problems in Cell Differentiation Vol. 13: Germ line-Soma Differentiation, ed. W. Henning, Springer-Verlag, pp71-104.
17. Gibbons I. R., Cosson M. P., Evans J. A., Gibbons B. H., Houck B., Martinson K. H., Sale W. S. and Tang, W. J. (1978). Potent inhibition of dynein adenosinetriphosphatase and of the motility of cilia and sperm flagella by vanadate. Proc. Nat. Acad. Sci. USA 75, 2220-2224.
18. Heald, R., Tournebize, R., Blank, T., Sandaltzopoulos, R., Becker, P., Hyman, A. and Karsenti, E. (1996). Self-organization of microtubules into bipolar spindles around artificial chromosomes in Xenopus egg extracts. Nature 382, 420-425.
19. Heald, R., Tournebize, R., Habermann, A., Karsenti, E. and Hyman, A. (1997). Spindle assembly in Xenopus egg extracts: Respective roles of centrosomes and microtubule self-organization. J. Cell Biol. 138, 615-628.
20. Heath, I. B. (1980). Variant mitosis in lower eukaryotes: indicators of the evolution of mitosis? Int. Rev. Cytol. 64, 1-80.
21. Hyman, A., Drechsel, D., Kellogg, D., Salser, S., Sawin, K., Steffen, P., Wordeman, L. and Mitchison, T. (1991). Preparation of modified tubulin. Meth. Enzymol. 196, 478-485.
22. Inoue, S. (1964). Organization and function of the mitotic spindle. In Primitive Motile Systems (ed. R. D. Allen and N. Kamiya), pp. 549-594. Academic Press, NY.
23. Inoue, S. (1981). Cell division and the mitotic spindle. J. Cell Biol. 91, 131-147s.
24. Inoue, S. and Sato, H. (1967). Cell motility by labile association of molecules: The nature of mitotic spindle fibers and their role in chromosome movement. J. Gen. Physiol. 50, 259-292.
25. Inoue, S., Yoder, O. C., Turgeon, B. G. and Aist, J. R. (1998). A cytoplasmic dynein required for mitotic aster formation in vivo. J. Cell Sci. 111, 2607-2614.
26. Ito, K., Masuda, M., Fujiwara, K., Hiroshi, H. and Sato, H. (1994). Metaphase and anaphase in the artificially induced monopolar spindle. Proc. Nat. Acad. Sci. USA 91, 3921-3925.
27. Kirschner, M. and Mitchison, T. (1986). Beyond self-assembly: from microtubules to morphogenesis. Cell 45, 329-342.
28. Kubai, D. F. (1975). The evolution of the mitotic spindle. Int. Rev. Cytol. 43, 167-227.
29. Kuriyama, R., Borisy, G. G. and Masui, Y. (1986). Microtubule cycle in oocytes of the surf clam, Spisula solidissima: An immunofluorescence study. Dev. Biol. 114, 151-160.
30. Laemmli, U. K. (1970). Cleavage of structural proteins during the assembly of the head bacteriophage T4. Nature 227, 680-685.
31. Lohka, M. J. and Mahler, J. L. (1985). Induction of nuclear envelope breakdown, chromosome condensation, and spindle formation in cell-free extracts. J. Cell Biol. 101, 518-523.
32. Longo, F. J. and Anderson, E. (1970). An ultrastructural analysis of fertilization in the surf clam, Spisula solidissima. H. Development of the male pronucleus and the association of the maternally and paternally derived chromosomes. J. Utrastruct. Res. 33, 495-514.
33. Matheis, H. J. G., McDonald, H., Goldstein, L. S. B. and Theurkauf, W. E. (1996). Anastral meiotic spindle morphogenesis: Role of the non-claret disjunctional kinesin-like protein. J. Cell Biol. 134, 455-464.
34. Mazia, D. and Dan, K. (1952). The isolation and biochemical characterization of the mitotic apparatus of dividing cells. Proc. Nat. Acad. Sci. USA 38, 826-838.
35. Mazia, D., Harris, P. J. and Bibring, T. (1960). The multiplicity of the mitotic centers and the time-course of their duplication and separation. J. Biophys. Biochem. Cytol. 7, 1-20.
36. Mazia, D. (1987). The chromosome cycle and the centrosome cycle in the mitotic cycle. Int. Rev. Cytol. 100, 49-92.
37. Merdes, A. and Cleveland, D. W. (1997). Pathways of spindle pole formation: different mechanisms conserved components. J. Cell Biol. 138, 953-956.
38. Merdes, A., Ramyar, K., Vechio, J. D. and Cleveland, D. W. (1996). A complex of NuMA and cytoplasmic dynein is essential for mitotic spindle assembly. Cell 87, 447-458.
39. McIntosh, J. R. (1983). The centrosome as organizer of the cytoskeleton. Mod. Cell Biol. 2, 115-142.
40. McIntosh, J. R. and Koonce, M. P. (1989). Mitosis. Science 246, 622-628.
41. McKim, K. S. and Hawley, R. S. (1995). Chromosomal control of meiotic cell division. Science 270, 1595-1601.
42. McNiven, M. A., Wang, M. and Porter, K. R. (1984). Microtubule polarity and the direction of pigment transport reverse simultaneously in surgically severed melanophore arms. Cell 37, 753-765.
43. Mitchison, T. J. (1989). Mitosis; basic concepts. Curr. Opin. Cell Biol. 1, 67-74.
44. Mitchison, T. J. and Kirschner, M. (1984). Microtubule assembly nucleated by isolated centrosomes. Nature 312, 232-237.
45. Mitchison, T. J. and Kirschner, M. (1986). Isolation of mammalian centrosomes. Meth. Enzymol. 134, 261-168.
46. Murray, A. W. (1991). Cell cycle extracts. Meth. Cell Biol. 36, 581-605.
47. Murray, A. W., Desai, A. B. and Salmon, E. D. (1996). Real time observation of anaphase in vitro. Proc. Nat. Acad. Sci. USA 93, 12327-12332.
48. Palazzo, R. E., Brawley, J. B. and Rebhun, L. I. (1988). Spontaneous aster formation in cytoplasmic extracts from eggs of the surf clam. Zoolog. Sci. (Japan) 5, 603-611.
49. Palazzo, R. E., Vaisberg, E., Cole, R. W. and Rieder, C. L. (1992). Centriole duplication in lysates of Spisula solidissima oocytes. Science 256, 219-221.
50. Palazzo, R. E. and Vogel, J. V. (1999). Isolation of centrosomes from Spisula solidissima oocytes. In Mitosis and Meiosis: Methods in Cell Biology, vol. 61 (ed. C. L. Rieder), pp. 35-56. Academic Press Inc.
51. Pfarr, C. M., Cove, M., Grissom, P. M., Hays, T. S., Porter, M. E. and McIntosh, J. R. (1990). Cytoplasmic dynein is localized to kinetochores during mitosis. Nature 345, 263-265.
52. Picket-Heaps, J., Tippit, D. H. and Porter, K. R. (1982). Rethinking mitosis. Cell 29, 729-744.
53. Porter M. E., Scholey J. M., Stemple D. L., Vigers G. P., Vale R. D., Sheetz M. P. and McIntosh J. R. (1987). Characterization of the microtubule movement produced by sea urchin egg kinesin. J. Biol. Chem. 262, 2794-2802.
54. Rebhun, L. I. (1959). Studies of early cleavage in the surf clam. Spisula solidissima, using methylene blue and toluidine blue as vital stains. Biol. Bull. 117, 518-545.
55. Rebhun, L. I. and Sharpless, T. K. (1964). Isolation of spindles from the surf clam Spisula solidissima. J. Cell Biol. 22, 488-492.
56. Rieder, C. L. and Alexander, S. P. (1990). Kinetochores are transported poleward along a single astral microtubule during chromosome attachment to the spindle in newt lung cells. J. Cell Biol. 110, 81-95.
57. Rieder, C. L. (1991). Towards a molecular understanding of chromosome behavior. Curr. Opin. Cell Biol. 3, 59-66.
58. Rodionov, V. I. and Borisy, G. G. (1997). Self-centering activity of cytoplasm. Nature 386, 170-173.
59. Ruderman, J. V., Sudakin, V. and Hershko, A. (1997). Preparation of clam oocyte extracts for cell cycle studies. Meth. Enzymol. 283, 614-622.
60. Sachs, M. (1971). A cytological analysis of artificial parthenogenesis in the surf clam Spisula solidissima. J. Ultrastruct. Res. 36, 806-823.
61. Sawin, K. E. and Mitchison, T. J. (1991). Mitotic spindle assembly by two different pathways in vitro. J. Cell Biol. 112, 925-940.
62. Sawin, K. E., Lefuellec, K., Philippe, M. and Mitchison, T. J. (1992). Mitotic spindle organization by a plus-end-directed microtubule motor. Nature 359, 540-543.
63. Schrader, F. (1953). Mitosis. Second Edition. Columbia University Press.
64. Shpetner, H. S., Paschal, B. M. and Vallee, R. B. (1988). Characterization of the microtubule-activated ATPase of brain cytoplasmic dynein (MAP 1C). J. Cell Biol. 107, 1001-1009.
65. Sluder, G. and Rieder, C. L. (1985). Experimental separation of pronuclei in fertilized sea urchin eggs: chromosomes do not organize a spindle in the absence of centrasomes. J. Cell Biol. 100, 897-903.
66. Steffen, W., Fuge, H., Dietz, R., Bastmeyer, M. and Müller, G. (1986). Aster-free spindle poles in insect spermatocytes: Evidence for chromosome-induced spindle formation. J. Cell Biol. 102, 1679-1687
67. Steffen, W., Hodgkinson, J. L. and Wiche, G. (1996). Immunogold-localization of the intermediate chain within the protein complex of cytoplasmic dynein. J. Struct. Biol. 117, 227-235.
68. Steffen, W., Karki, S., Vaughan, K. T., Vallee, R. B., Holzbaur, L. E., Weiss, D. G. and Kuznetsov, S. A. (1997). The 74-kD intermediate chain of cytoplasmic dynein is involved in binding the motor to membranous organelles. Mol. Biol. Cell 8, 2077-2088.
69. Steuer, E. R., Wordeman, L., Schroer, T. A. and Sheetz, M. P. (1990). Localization of cytoplasmic dynein to mitotic poles and kinetochores. Nature 345, 266-268.
70. Suprenant, K. A. (1991). Unidirectional microtubule assembly in cell-free extracts of Spisula solidissima oocytes is regulated by subtle changes in pH. Cell Motil. Cytoskel. 19, 207-220.
71. Theurkauf, W. E. and Hawley, R. S. (1992). Meiotic spindle assembly in Drosophila females: behavior of nonexchange chromosomes and the effects of mutations in the nod kinesin-like protein. J. Cell Biol. 116, 1167-1180.
72. Towbin, H., Staehelin, T. and Gordon, J. (1979). Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some application. Proc. Nat. Acad. Sci. USA 76, 4350-4354.
73. Vaisberg, E. A., Grissom, G. M. and McIntosh, J. R. (1993). Cytoplasmic dynein plays a role in mammalian mitotic spindle formation. J. Cell Biol. 123, 849-858.
74. Verde, F., Berrez, J. M., Antony, C. and Karsenti, E. (1991). Taxol-induced microtubule asters in mitotic extracts of Xenopus eggs: requirement for phosphorylated factors and cytoplasmic dynein. J. Cell Biol. 112, 1177-1187.
75. Vogel, J. M., Stearns, T., Rieder, C. L. and Palazzo, R. E. (1997). Centrosomes isolated from Spisula solidissima oocytes contain rings and an unusual stoichiometric ratio of α/β tubulin. J. Cell Biol. 137, 193-202.
76. Waters, J. C. and Salmon, E. D. (1997). Pathways of spindle assembly. Curr. Opin. Cell Biol. 97, 37-43.
77. Wilson, E. B. (1925). The Cell in Development and Heredity. 3rd edn. Macmillan Co.
78. Weisenberg, R. C. and Rosenfeld, A. C. (1975). In vitro polymerization of microtubules into asters and spindles in homogenates of surf clam eggs. J. Cell Biol. 64, 146-158.
79. Weiss, D. G., Maile, W. and Wick, R. A. (1989). Video microscopy. In Light Microscopy in Biology. A Practical Approach (ed. A. J. Lacey), pp. 221-278. IRL Press Oxford.
80. Zhang D. and Nicklas, R. B. (1995). The impact of chromosomes and centrosomes on spindle assembly as observed in living cells. J. Cell Biol. 129, 1287-1300.