Chapter 3 Isolation of Centrosomes from Spisula solidissima Oocytes

Methods in Cell Biology

Volumn 61, Issue C, 1998, Pages 35-56

  Palazzo, Robert E ^a,b   Vogel, Jacalyn M ^a,b  

^a UNIVERSITY OF KANSAS   (United States)

^b MARINE BIOLOGICAL LABORATORY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

MICROTUBULE ASSOCIATED PROTEIN;

ANIMAL; CELL FRACTIONATION; CENTROSOME; FLUORESCENT ANTIBODY TECHNIQUE; IMMUNOLOGY; ISOLATION AND PURIFICATION; METHODOLOGY; MOLLUSC; OOCYTE; REVIEW; ULTRASTRUCTURE;

ANIMALS; CELL FRACTIONATION; CENTROSOME; FLUORESCENT ANTIBODY TECHNIQUE; MICROTUBULE-ASSOCIATED PROTEINS; MOLLUSCA; OOCYTES;

EID: 0032607864     PISSN: 0091679X     EISSN: None     Source Type: Book Series    
DOI: 10.1016/S0091-679X(08)61974-3     Document Type: Article

References (17)

1. Allen, R.D., Fertilization and artificial activation in the egg of the surf clam Spisula solidissima. Biol. Bull 105 (1953), 213-219.
2. Bornens, M., Paintrand, M., Berges, J., Marty, M.C., Karsenti, E., Structural and chemical characterization of isolated centrosomes. Cell Motil. Cytoskel 8 (1987), 238-249.
3. Costello, D.P., Henley, C., Methods for Obtaining and Handling Marine Eggs and Embryos, 1971, Marine Biological Laboratory, Woods Hole, MA.
4. Mitchison, T.J., Kirschner, M., Microtubule assembly nucleated by isolated centrosomes. Nature (London) 312 (1984), 232-237.
5. Mitchison, T.J., Kirschner, M., Isolation of mammalian centrosomes. Methods Enzymol 134 (1986), 261-268.
6. Moritz, M., Braunfield, M.B., Fung, J.C., Sedat, J.W., Alberts, B.M., Agard, D.A., Three-dimensional structural characterization of centrosomes from early Drosophila embryos. J. Cell Biol 130 (1995), 1149-1159.
7. Moritz, M., Braunfield, M.B., Sedat, J.W., Alberts, B.M., Agard, D.A., Microtubule nucleation by γ-tubulin containing rings in the centrosome. Nature (London) 378 (1995), 638-640.
8. Palazzo, R.E., Brawley, J.B., Rebhun, L.I., Spontaneous aster formation in cytoplasmic extracts from eggs of the surf clam. Zool. Sci. (Tokyo) 5 (1988), 603-611.
9. Palazzo, R.E., Lutz, D.A., Rebhun, L.I., Reactivation of isolated mitotic apparatus: Metaphase versus anaphase spindles. Cell. Motil. Cytoskel 18 (1991), 304-318.
10. Palazzo, R.E., Vaisberg, E., Cole, R.W., Rieder, C.L., Centriole duplication in lysates of Spisula solidissima oocytes. Science (Washington, DC) 256 (1992), 219-221.
11. Rebhun, L.I., Sharpless, T.K., Isolation of spindles from the surf clam Spisula solidissima. J. Cell Biol 22 (1964), 488-491.
12. Rieder, C.L., Rupp, G., Bowser, S.S., Electron microscopy of semithick sections: Advantages for biomedical research. J. Electron Microsc. Technol 2 (1985), 11-28.
13. Rout, M.P., Kilmartin, J.V., Components of the yeast spindle and spindle pole body. J. Cell. Biol 111 (1990), 1913-1927.
14. Schechter, V., Experimental studies upon the egg cells of the clam, Mactra solidissima, with special reference to longevity. J. Exp. Zool 86 (1941), 461-477.
15. Suprenant, K.A., Foltz-Dagget, M.A., Sea urchin microtubules. Curr. Topics Dev. Biol 31 (1995), 65-99.
16. Suprenant, K.A., Marsh, J.C., Temperature and pH govern the self-assembly of microtubules from unfertilized sea urchin egg extracts. J. Cell. Sci 87 (1987), 71-84.
17. Vogel, J.M., Stearns, T., Rieder, C.L., Palazzo, R.E., Centrosomes isolated from Spisula solidissima oocytes contain rings and an unusual stoichiometric ratio of α/β tubulin. J. Cell Biol 137 (1997), 193-202.