Development of improved pUB110-based vectors for expression and secretion studies in Bacillus subtilis

Journal of Biotechnology

Volumn 72, Issue 3, 1999, Pages 185-195

  Wu, Sau Ching ^a   Wong, Sui Lam ^a  

^a UNIVERSITY OF CALGARY   (Canada)

Author keywords

Bacillus subtilis; BirA; Expression vectors; PrsA; pUB110; Staphylokinase

Indexed keywords

BIOTECHNOLOGY; ENZYMES; GENES;

BACILLUS SUBTILIS; SECRETION; STAPHYLOKINASE;

BACTERIA;

PLASMID VECTOR; STAPHYLOKINASE; AUR PROTEIN, STAPHYLOCOCCUS AUREUS; BACTERIAL PROTEIN; BIRA PROTEIN, E COLI; ESCHERICHIA COLI PROTEIN; LIGASE; LIPOPROTEIN; MEMBRANE PROTEIN; METALLOPROTEINASE; PRSA PROTEIN, BACTERIA; REPRESSOR PROTEIN; TRANSCRIPTION FACTOR;

BACILLUS SUBTILIS; CONTROLLED STUDY; EXPRESSION VECTOR; GENE EXPRESSION; GENE INSERTION; GENETIC TRANSCRIPTION; MODEL; NONHUMAN; PLASMID; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN SECRETION; REVIEW; ARTICLE; BIOSYNTHESIS; GENE EXPRESSION REGULATION; GENE VECTOR; GENETICS; METABOLISM;

BACILLUS SUBTILIS;

BACILLUS SUBTILIS; BACTERIAL PROTEINS; CARBON-NITROGEN LIGASES; ESCHERICHIA COLI PROTEINS; GENE EXPRESSION REGULATION, BACTERIAL; GENETIC VECTORS; LIPOPROTEINS; MEMBRANE PROTEINS; METALLOENDOPEPTIDASES; REPRESSOR PROTEINS; TRANSCRIPTION FACTORS;

EID: 0032971539     PISSN: 01681656     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0168-1656(99)00101-7     Document Type: Article

References (56)

1. Behnke, D., 1992. Protein export and the development of secretion vectors. In: Doi, R.H., McGloughlin, M. (Eds.), Biology of Bacilli: Applications to industry. MA: Butterworth-Heinemann, pp. 143-188.
2. Behnke D., Gerlach D. Cloning and expression in Escherichia coli, Bacillus subtilis, and Streptococcus sanguis of a gene for staphylokinase-a bacterial plasminogen activator. Mol. Gen. Genet. 210:1987;528-534.
3. Blattner F.R., Plunkett G. et al. The complete genome sequence of Escherichia coli K-12. Science. 277:1997;1453-1474.
4. Bolhuis A., Broekhuizen C.P., Sorokin A., van Roosmalen M.L., Venema G., Bron S., Quax W.J., Van Dijl J.M. SecDF of Bacillus subtilis, a molecular Siamese twin required for the efficient secretion of proteins. J. Biol. Chem. 273:1998;21217-21224.
5. Bower S., Perkins J., Yocum R.R. et al. Cloning and characterization of the Bacillus subtilis birA gene encoding a repressor of the biotin operon. J. Bacteriol. 177:1995;2572-2575.
6. Bron, S., 1990. Plasmids. In: Harwood, C.R., Cutting, S.M. (Eds.), Molecular Biological Methods for Bacillus. Wiley, Chichester, pp. 75-174.
7. Bron S., Bolhuis A., Tjalsma H., Holsappel S., Venema G., Van Dijl J.M. Protein secretion and possible roles for multiple signal peptidases for precursor processing in bacilli. J. Biotechnol. 64:1998;3-13.
8. Collen D. Staphylokinase: a potent, uniquely fibrin-selective thrombolytic agent. Nat. Med. 4:1998;279-284.
9. Delihas N. Regulation of gene expression by trans-encoded antisense RNAs. Mol. Microbiol. 15:1995;411-414.
10. Duvall E.J., Williams D.M., Mongkolsuk S., Lovett P.S. Regulatory regions that control expression of two chloramphenicol-inducible cat genes cloned in Bacillus subtilis. J. Bacteriol. 158:1984;784-790.
11. Fahnestock S.R., Fisher K.E. Expression of the staphylococcal protein A gene in Bacillus subtilis by gene fusions utilizing the promoter from a Bacillus amyloliquefaciens alpha-amylase gene. J. Bacteriol. 165:1986;796-804.
12. Fahnestock S.R., Fisher K.E. Protease-deficient Bacillus subtilis host strains for production of staphylococcal protein A. Appl. Environ. Microbiol. 53:1987;379-384.
13. Grandi G., Del Bue M., Palla E., Mele A., Colletti E., Toma S. New plasmid expression vectors for Bacillus subtilis. Plasmid. 16:1986;1-14.
14. Halling S.M., Sanchez-Anzaldo F.J., Fukuda R., Doi R.H., Meares C.F. Zinc is associated with the beta subunit of DNA dependent RNA polymerase of Bacillus subtilis. Biochemistry. 16:1977;2880-2884.
15. Harwood C.R. Bacillus subtilis and its relatives: molecular biological and industrial workhorses. Trends Biotech. 10:1992;247-256.
16. He X.-S., Shyu Y.-T., Nathoo S., Wong S.-L., Doi R.H. Construction and use of a Bacillus subtilis mutant deficient in multiple protease genes for the expression of eucaryotic genes. Ann. NY Acad. Sci. 646:1991;69-77.
17. Jacobs M., Anderson J.B., Kontinen V., Sarvas M. Bacillus subtilis PrsA is required in vivo as an extracytoplasmic chaperone for secretion of active enzymes synthesized either with or without pro-sequences. Mol. Microbiol. 8:1993;957-966.
18. Jannière, L., Gruss, A., Ehrlich, S.D., 1993. Plasmids. In: Sonenshein, A.L., Hoch, J.A., Losick, R. (Eds.), Bacillus subtilis and other Gram-Positive Bacteria. Washington D.C. Am. Soc. Microbiol. pp. 625-644.
19. Kawamura F., Doi R.H. Construction of a Bacillus subtilis double-mutant deficient in extracellular alkaline and neutral proteases. J. Bacteriol. 160:1984;442-444.
20. Keggins K.M., Lovett P.S., Duvall E.J. Molecular cloning of genetically active fragments of Bacillus DNA in Bacillus subtilis and properties of the vector plasmid pUB110. Proc. Natl. Acad. Sci. U.S.A. 75:1978;1423-1427.
21. Kontinen V.P., Saris P., Sarvas M. A gene (prsA) of Bacillus subtilis involved in a novel, late stage of protein export. Mol. Microbiol. 5:1991;1273-1283.
22. Kunst F., Ogasawara N. et al. The complete genome sequence of the Gram-positive bacterium Bacillus subtilis. Nature. 390:1997;249-256.
23. Li M., Wong S.-L. Cloning and characterization of the groESL operon from Bacillus subtilis. J. Bacteriol. 174:1992;3981-3992.
24. Lin X., Wong S.-L., Miller E.S., Shih J.C. Expression of the Bacillus licheniformis PWD-1 keratinase gene in B. subtilis. J. Ind. Microbiol. Biotechnol. 19:1997;134-138.
25. Lovett, P.S., Ambulos, N.P, 1989. Genetic manipulation of Bacillus subtilis. In: Harwood, C.R. (Ed.), Bacillus. Plenum Press, New York, pp. 115-154.
26. McKenzie T., Hoshino T., Tanaka T., Sueoka N. The nucleotide sequence of pUB110: some salient features in relation to replication and its regulation. Plasmid. 15:1986;93-103.
27. Nagarajan, V., 1993. Protein secretion. In: Sonenshein, A.L., Hoch, J.A., Losick, R. (Eds.), Bacillus subtilis and other gram-positive bacteria. Washington, D.C. American Society for Microbiology, pp. 713-726.
28. Norrander J., Kempe T., Messing J. Construction of improved M13 vectors using oligodeoxynucleotide-directed mutagenesis. Gene. 26:1983;101-106.
29. Pero, J., Sloma, A., 1993. Proteases. In: Sonenshein, A.L., Hoch, J.A., Losick, R. (Eds.), Bacillus subtilis and other gram-positive bacteria, Biochemistry, physiology, and molecular genetics. Washington, D.C. American Society for Microbiology, pp. 939-952.
30. Peschke U., Beuck V., Bujard H., Gentz R., Le Grice S. Efficient utilization of Escherichia coli transcriptional signals in Bacillus subtilis. J. Mol. Biol. 186:1985;547-555.
31. Sarvas M. Gene expression in recombinant Bacillus. Bioprocess. Technol. :53-120. 22:1995;53-120.
32. Selinger L.B., McGregor N.F., Khachatourians G.G., Hynes M.F. Mobilization of closely related plasmids pUB110 and pBC16 by Bacillus plasmid pXO503 requires trans-acting open reading frame beta. J. Bacteriol. 172:1990;3290-3297.
33. Stahl M.L., Ferrari E. Replacement of the Bacillus subtilis subtilisin structural gene with an in vitro-derived deletion mutation. J. Bacteriol. 158:1984;411-418.
34. Takamatsu H., Bunai K., Horinaka T., Oguro A., Nakamura K., Watabe K., Yamane K. Identification of a region required for binding to presecretory protein in Bacillus subtilis Ffh, a homologue of the 54-kDa subunit of mammalian signal recognition particle. Eur. J. Biochem. 248:1997;575-582.
35. Takano T., Fukuda M., Monma M., Kobayashi S., Kainuma K., Yamane K. Molecular cloning, DNA nucleotide sequencing, and expression in Bacillus subtilis cells of the Bacillus macerans cyclodextrin glucanotransferase gene. J. Bacteriol. 166:1986;1118-1122.
36. Tjalsma H., Noback M.A., Bron S., Venema G., Yamane K., Van Dijl J.M. Bacillus subtilis contains four closely related type I signal peptidases with overlapping substrate specificities. Constitutive and temporally controlled expression of different sip genes. J. Biol. Chem. 272:1997;25983-25992.
37. Tsukagoshi N., Tabata R., Takemura T., Yamagata H., Udaka S. Molecular cloning of a major cell wall protein gene from protein-producing Bacillus brevis 47 and its expression in Escherichia coli and Bacillus subtilis. J. Bacteriol. 158:1984;1054-1060.
38. Wagner E.G., Simons R.W. Antisense RNA control in bacteria, phages, and plasmids. Annu. Rev. Microbiol. 48:1994;713-742.
39. Wang L.-F., Wong S.-L., Lee S.-G., Kalyan N.K., Hung P.P., Hilliker S., Doi R.H. Expression and secretion of human atrial natriuretic α-factor in Bacillus subtilis using the subtilisin signal peptide. Gene. 69:1988;39-47.
40. Wang L.-F., Hum W.T., Kalyan N.K., Lee S.G., Hung P.P., Doi R.H. Synthesis and refolding of human tissue-type plasminogen activator in Bacillus subtilis. Gene. 84:1989;127-133.
41. Wang L.-F., Doi R.H. Promoter switching during development and the termination site of the sigma 43 operon of Bacillus subtilis. Mol. Gen. Genet. 207:1987;114-119.
42. Wang, L.-F, Doi, R.H., 1992. Heterologous gene expression in Bacillus subtilis. In: Doi, R.H., McGloughlin, M. (Eds.), Biology of Bacilli: Applications to Industry. Stoneham, MA: Butterworth-Heinemann, pp. 63-104.
43. 37 RNA polymerase holoenzymes during growth and stationary phases. J. Biol. Chem. 259:1984;8619-8625.
44. Weisblum B., Graham M.Y., Gryczan T., Dubnau D. Plasmid copy number control: isolation and characterization of high-copy-number mutants of plasmid pE194. J. Bacteriol. 137:1979;635-643.
45. Williams D.M., Duvall E.J., Lovett P.S. Cloning restriction fragments that promote expression of a gene in Bacillus subtilis. J. Bacteriol. 146:1981;1162-1165.
46. Wong S.-L., Wang L.-F., Doi R.H. Cloning and nucleotide sequence of senN, a novel Bacillus natto gene that regulates expression of extracellular protein genes. J. Gen. Microbiol. 134:1988;3269-3276.
47. Wong S.-L. Development of an inducible and enhancible expression and secretion system in Bacillus subtilis. Gene. 83:1989;215-223.
48. Wong S.-L., Ye R., Nathoo S. Engineering and production of streptokinase in a Bacillus subtilis expression-secretion system. Appl. Environ. Microbiol. 60:1994;517-523.
49. Wong S.-L. Advances in the use of Bacillus subtilis for the expression and secretion of heterologous proteins. Curr. Opin. Biotechnol. 6:1995;517-522.
50. Wong, S.-L., Wu, X.-C., Yang, L.-P., Ng, S.-C., Hudson, N., 1995. Production and purification of antibody using Bacillus subtilis as an expression host. In: Wang, H.Y., Imanaka, T. (Eds.), Antibody Expression and Engineering. Washington, DC: American Chemical Society, pp. 100-107.
51. Wu S.-C., Ye R., Wu X.-C., Ng S.-C., Wong S.-L. Enhanced secretory production of a single-chain antibody fragment from Bacillus subtilis by coproduction of molecular chaperones. J. Bacteriol. 180:1998;2830-2835.
52. Wu X.-C., Lee W., Tran L., Wong S.-L. Engineering a Bacillus subtilis expression-secretion system with a strain deficient in six extracellular proteases. J. Bacteriol. 173:1991;4952-4958.
53. Wu X.-C., Ng S.-C., Near R.I., Wong S.-L. Efficient production of a functional single-chain antidigoxin antibody fragment via an engineered Bacillus subtilis expression-secretion system. Bio/Technology. 11:1993;71-76.
54. Ye, R., Yang, L.P., Wong, S.-L., 1996. Construction of protease deficient Bacillus subtilis strains for expression studies: inactivation of seven extracellular protease and the intracellular LonA protease. In: Proc. International symposium on Recent Advances in Bioindustry 160-169.
55. Ye R., Kim J.H., Kim B.G., Szarka S., Sihota S., Wong S.-L. High-level secretory production of intact, biologically active staphylokinase from Bacillus subtilis. Biotechnol. Bioeng. 62:1999;87-96.
56. Zyprian E., Matzura H. Characterization of signals promoting gene expression on the Staphylococcus aureus plasmid pUB110 and development of a gram-positive expression vector system. DNA. 5:1986;219-225.