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Katanin, a microtubule-severing protein, is a novel AAA ATPase that targets to the centrosome using a WD40-containing subunit
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The authors report the cloning and characterization of the two katanin subunits. The p60 subunit has ATPase and microtubule severing activities and the p80 subunit has WD40 repeats and targets the protein to centrosomes.
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Hartman JJ, Mahr J, McNally K, Okawa K, Iwamatsu A, Thomas S, Cheesman S, Heuser J, Vale RD, McNally FJ Katanin, a microtubule-severing protein, is a novel AAA ATPase that targets to the centrosome using a WD40-containing subunit. Cell. 93:1998;277-287. The authors report the cloning and characterization of the two katanin subunits. The p60 subunit has ATPase and microtubule severing activities and the p80 subunit has WD40 repeats and targets the protein to centrosomes.
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The evidence presented here demonstrates that centrosome replication in sea urchin zygotes is independent of the levels of Cdk1- cyclin B activity but appears to require an S phase activity to 'license' duplication in the subsequent cell cycle.
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Requirement for cdk2-cyclin E activity for repeated centrosome reproduction in Xenopus eggs
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in press. Using microtubule aster doublings to monitor centrosome duplication in S-phase-arrested Xenopus extracts, the authors conclude that duplication is blocked by addition of the ckd2-cyclin E inhibitor, Δ34Xic1 and restored by addition of purified cdk2 - cyclin E. They show that cyclin E is localized to centrosomes by immunofluorescence microscopy.
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Hinchcliffe, E.H.1
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Cyclin-dependent kinase control of centrosome duplication
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in press. To analyze centrosome duplication, the authors monitor the number of microtubule asters and γ tubulin spots in Xenopus embryos and the separation of centrioles by deconvolution microscopy in Xenopus extracts. They conclude that centrosome duplication is reduced by injection of the cdk inhibitors p21 and p27 into Xenopus extracts and restored by adding back cdk2-cyclin E protein. Depletion of cyclin E or cyclin A from extracts also eliminates centriole separation.
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Lacey KR, Jackson PK, Stearns T Cyclin-dependent kinase control of centrosome duplication. Proc Natl Acad Sci USA. 1999;. in press. To analyze centrosome duplication, the authors monitor the number of microtubule asters and γ tubulin spots in Xenopus embryos and the separation of centrioles by deconvolution microscopy in Xenopus extracts. They conclude that centrosome duplication is reduced by injection of the cdk inhibitors p21 and p27 into Xenopus extracts and restored by adding back cdk2-cyclin E protein. Depletion of cyclin E or cyclin A from extracts also eliminates centriole separation.
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The bimC family of kinesins: Essential bipolar mitotic motors driving centrosome separation
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A centrosomal function for the human Nek2 protein kinase, a member of the NIMA family of cell cycle regulators
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This study shows that overexpression of the centrosome-associated kinase NEK2 leads to splitting of centrosomes and fragmentation of pericentriolar material.
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Fry AM, Meraldi P, Nigg EA A centrosomal function for the human Nek2 protein kinase, a member of the NIMA family of cell cycle regulators. EMBO J. 17:1998;470-481. This study shows that overexpression of the centrosome-associated kinase NEK2 leads to splitting of centrosomes and fragmentation of pericentriolar material.
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