A near-native state on the slow refolding pathway of hen lysozyme

Protein Science

Volumn 8, Issue 1, 1999, Pages 35-44

  Kulkarni, Sunita K ^a   Ashcroft, Alison E ^a   Carey, Michael ^b   Masselos, Dimitris ^c   Robinson, Carol V ^c   Radford, Sheena E ^a  

^a UNIVERSITY OF LEEDS   (United Kingdom)

^b Applied Pholophysics Ltd   (United Kingdom)

^c UNIVERSITY OF OXFORD   (United Kingdom)

Author keywords

Hydrogen exchange; Ionic strength; Lysozyme; Mass spectrometry; Protein refolding

Indexed keywords

ACETIC ACID; DISULFIDE; ENZYME INHIBITOR; LYSOZYME; POTASSIUM CHLORIDE; POTASSIUM DERIVATIVE; POTASSIUM SULFATE; THIOCYANATE POTASSIUM; TRYPTOPHAN; UNCLASSIFIED DRUG;

ARTICLE; FLUORESCENCE; HEN; IONIC STRENGTH; MASS SPECTROMETRY; NONHUMAN; PRIORITY JOURNAL; PROTEIN DOMAIN; PROTEIN FOLDING;

EID: 0032923553     PISSN: 09618368     EISSN: None     Source Type: Journal    
DOI: 10.1110/ps.8.1.35     Document Type: Article

References (47)

1. Bai Y, Milne JS, Mayne L, Englander SW. 1993. Primary structure effects on peptide group hydrogen exchange. Proteins Struct Funct Genet 17:75-86.
2. Baldwin RL. 1996. How Hofmeister ion interactions affect protein stability. Biophys J 71:2056-2063.
3. Blake CCF, Koenig DF, Mair GA, Sarma R. 1965. Crystal structure of lysozyme by X-ray diffraction. Nature 206:757-761.
4. Blake CCF, Pulford WCA, Artymuik PJ. 1983. X-ray studies of water in crystals of lysozyme. J Mol Biol 167:365-377.
5. Booth DR, Sunde M, Bellotti V, Robinson CV, Hutchinson WL, Fraser PE, Hawkins PN, Dobson CM, Radford SE, Blake CCF, Pepys MB. 1997. Instability, unfolding and aggregation of human lysozyme variants underlying amyloid fibrillogenesis. Nature 385:787-793.
6. Chaffotte A, Guillou Y, Goldberg ME. 1992. Kinetic resolution of peptide bond and side chain far-UV circular dichroism during the folding of hen egg white lysozyme. Biochemistry 31:9694-9702.
7. Dao-pin S, Liao D, Remington SJ. 1989. Electrostatic fields in the active sites of lysozymes. Proc Natl Acad Sci USA 86:5361-5365.
8. Devereux J, Haeberli P, Smithies D. 1984. A comprehensive set of sequence analysis programs for the VAX. Nucleic Acid Res 12:387-395.
9. Dill AK, Chan HS. 1997. From Levinthal to pathways to funnels. Nature Struct Biol 4:10-19.
10. Dobson CM, Evans PA, Radford SE. 1994. Understanding how proteins fold: The lysozyme story so far. Trends Biochem Sci 19:31-37.
11. Dobson CM, Sali A, Karplus M. 1998. Protein folding: A perspective from theory and experiment. Angew Chem 37:868-893.
12. Fink AL, Calciano LJ, Goto Y, Kurotsu Y, Palleros DR. 1994. Classification of acid denaturation of proteins: Intermediates and unfolded states. Biochemistry 33:12504-12511.
13. Gladwin ST, Evans PA. 1996. Structure of very early protein folding intermediates: New insights through a variant of hydrogen exchange labelling. Fold Design 1:407-417.
14. Goto Y, Calciano LJ, Fink AL. 1990a. Acid-induced folding of proteins. Proc Natl Acad Sci USA 87:573-577.
15. Goto Y, Takahashi M, Fink AL. 1990b. Mechanism of acid-induced unfolding of proteins. Biochemistry 29:3480-3488.
16. Imoto T, Johnson LN, North ACT, Philips DC, Rupley JA. 1972. Vertebrate lysozymes. In: Boyer PD, ed. Vertebrate lysozymes. New York: Academic Press. pp 666-868.
17. Itzhaki LS, Evans PA. 1996. Solvent isotope effects on the refolding kinetics of hen egg-white lysozyme. Protein Sci 5:140-146.
18. Itzhaki LS, Evans PA, Dobson CM, Radford SE. 1994. Tertiary interactions in the folding pathway of hen lysozyme: Kinetic studies using fluorescent probes. Biochemistry 33:5212-5220.
19. Jollés P, Jollés J. 1984. What's new in lysozyme research? Mol Cell Biochem 63:165-189.
20. Kabsch W, Sander C. 1983. Dictionary of protein secondary structure pattern recognition of hydrogen bonded and geometrical features. Biopolymers 12:2577-2637.
21. Kato S, Okamura M, Shimamoto N, Utiyama H. 1981. Spectral evidence for a rapidly formed structural intermediate in the refolding kinetics of hen egg white lysozyme. Biochemistry 20:1080-1085.
22. Kato S, Shimamoto N, Utiyama H. 1982. Identification and characterisation of the direct folding process of hen egg white lysozyme. Biochemistry 21: 38-43.
23. Kiefhaber T. 1995. Kinetic traps in lysozyme folding. Proc Natl Acad Sci USA 92:9029-9033.
24. Kraulis PJ. 1991. Molscript: A program to produce both detailed and schematic plots of protein structures. J Appl Cryst 24:946-950.
25. Lazaridis T, Karplus M. 1997. "New view" of protein folding reconciled with the old through multiple unfolding simulations. Science 278:1928-1931.
26. Matagne A, Chung EW, Ball LJ, Radford SE, Robinson CV, Dobson CM. 1998. The origin of the α-domain intermediate in the folding of hen lysozyme. J Mol Biol 277:997-1005.
27. Matagne A, Radford SE, Dobson SE. 1997. Fast and slow tracks in lysozyme folding: Insight into the role of domains in the folding process. J Mol Biol 267:1068-1074.
28. McCrary BS, Bedell J, Edmondson SP, Shriver JW. 1998. Linkage of protonation and anion binding to the folding of Sac7d. J Mol Biol 276:203-224.
29. McKenzie HA, White FH. 1991. Lysozyme and α-lactalbumin: Structure, function and interrelationships. Adv Protein Chem 41:173-315.
30. Miranker A, Radford SE, Karplus M, Dobson CM. 1991. An NMR demonstration of folding domains in lysozyme. Nature 349:633-636.
31. Miranker A, Robinson CV, Radford SE, Aplin RT, Dobson CM. 1993. Detection of transient protein folding populations by mass spectrometry. Science 262:896-899.
32. Miranker A, Robinson CV, Radford SE, Dobson CM. 1996. Investigation of protein folding by mass spectrometry. FASEB J 10:93-101.
33. Morgan CJ, Miranker A, Dobson CM. 1998. Characterisation of collapsed states in the early stages of the refolding of hen lysozyme. Biochemistry 37:8473-8480.
34. Moult J, Yonath A, Traub W, Smilansky A, Podjarny A, Rabinovich D, Saya A. 1976. The structure of triclinic lysozyme at 2.5 Å resolution. J Mol Biol 100:179-195.
35. Pace CN. 1986. Determination and analysis of urea and guanidine hydrochloride denaturation curves. Methods Enzymol 131:266-280.
36. Pepys MB, Hawkins PN, Booth DR, Vigushin DM, Tennent GA, Soutar AK, Totty N, Nguyen O, Blake CCF, Terry CJ, Feest TG, Zalin AM, Hsuan JJ. 1993. Human lysozyme gene mutations cause hereditary systemic amyloidosis. Nature 362:553-557.
37. Radford SE, Dobson CM. 1995. Insights into protein folding using physical techniques. Proc Roy Soc London B 348:17-25.
38. Radford SE, Dobson CM, Evans PA. 1992. The folding of hen lysozyme involves partially structured intermediates and multiple pathways. Nature 358:302-307.
39. Rothwarf DM, Scheraga HA. 1996. Role of non-native aromatic and hydrophobic interactions in the folding of hen egg white lysozyme. Biochemistry 35:13797-13807.
40. Shih P, Holland DR, Kirsch JF. 1995. Thermal stability determinants of chicken egg-white lysozyme core mutants: hydrophobicity, packing volume and conserved buried water molecules. Protein Sci 4:2050-2062.
41. Timasheff SN. 1993. The control of protein stability and association by weak interactions with water: How do solvents affect these processes? Annu Rev Biophys Biomolec Struct 22:67-97.
42. von Hippel PH, Schleich T. 1969. The effects of neutral salts on the structure of biological macromolecules. In: Timasheff SN, Fasman GD, eds. The effects of neutral salts on the structure of biological macromolecules. New York: Marcel Dekker Inc. pp 417-574.
43. Washabaugh MW, Collins KD. 1986. The systematic characterisation by aqueous column chromatography of solutes which affect protein stability. J Biol Chem 261:12477-12485.
44. Wildegger G, Kiefhaber T. 1997. Three state model for lysozyme folding: Triangular folding mechanism with an energetically trapped intermediate. J Mol Biol 270:294-304.
45. Williams MA, Goodfellow JM, Thomton JM. 1994. Buried waters and internal cavities in monomeric proteins. Protein Sci 3:1224-1235.
46. Wolynes PG, Luthey-Schulten Z, Onuchic JN. 1996. Fast folding experiments and the topography of protein folding energy landscapes. Chem Biol 3:425-432.
47. Yang JJ, Buck M, Pitkeathly M, Kolik M, Haynie DT, Dobson CM, Radford SE. 1995. Conformational properties of four peptides spanning the sequence of hen lysozyme. J Mol Biol 252:483-491.