Demonstration of a dynamic, transcription-dependent organization of pre-mRNA splicing factors in polytene nuclei

Journal of Cell Biology

Volumn 133, Issue 5, 1996, Pages 929-941

  Baurén, Göran ^a   Jiang, Wei Qin ^a   Bernholm, Kerstin ^a   Gu, Feng ^a   Wieslander, Lars ^a  

^a KAROLINSKA INSTITUTE   (Sweden)

Author keywords

[No Author keywords available]

Indexed keywords

CHIRONOMUS TENTANS; DIPTERA; ANIMALIA;

MESSENGER RNA PRECURSOR; TRANSCRIPTION FACTOR;

ANIMAL CELL; ARTICLE; CONTROLLED STUDY; FLY; GENE LOCUS; MESSENGER RNA SYNTHESIS; NONHUMAN; PRIORITY JOURNAL; RNA SPLICING; TRANSCRIPTION INITIATION; TRANSCRIPTION REGULATION;

ANIMALS; CELL NUCLEUS; CHIRONOMIDAE; CHROMATIN; DACTINOMYCIN; DICHLORORIBOFURANOSYLBENZIMIDAZOLE; GALACTOSE; GENE EXPRESSION REGULATION; PROTEIN SYNTHESIS INHIBITORS; RIBONUCLEOPROTEIN, U2 SMALL NUCLEAR; RNA PRECURSORS; RNA SPLICING; SALIVARY GLANDS; SPLICEOSOMES; TRANSCRIPTION, GENETIC;

EID: 0029973772     PISSN: 00219525     EISSN: None     Source Type: Journal    
DOI: 10.1083/jcb.133.5.929     Document Type: Article

References (66)

1. Antoniou, M., M. Carmo-Fonseca, J. Ferreira, and A.I. Lamond. 1993. Nuclear organization of splicing snRNPs during differentiation of murine erythroleukemia cells in vitro. J. Cell Biol. 123:1055-1068.
2. Baurén, G., and L. Wieslander. 1994. Splicing of Balbiani ring 1 gene premRNA occurs simultaneously with transcription. Cell. 76:183-192.
3. Beermann, W. 1973. Directed changes in the pattern of Balbiani ring puffing in Chironomus: effects of sugar treatment. Chromosoma (Berl.). 41:297-326.
4. Beyer, A.L., and Y.N. Osheim. 1988. Splice site selection, rate of splicing and alternative splicing on nascent transcripts. Genes & Dev. 2:754-765.
5. Bridge, E., M. Carmo-Fonseca, A.I. Lamond, and U. Pettersson. 1993. Nuclear organization of splicing small nuclear ribonucleoproteins in adenovirus-infected cells. J. Virol. 67:5792-5802.
6. Bridge, E., D.-X. Xia, M. Carmo-Fonseca, B. Cardinali, A.I. Lamond, and U. Pettersson. 1995. Dynamic organization of splicing factors in adenovirus-infected cells. J. Virol. 69:281-290.
7. Carmo-Fonseca, M., M. Pepperkok, M.T. Carvalho, and A.I. Lamond. 1992. Transcription-dependent colocalization of the U1, U2. U4/U6, and U5 snRNPs in coiled bodies. J. Cell Biol. 117:1-14.
8. Carmo-Fonseca, M., J. Ferreira, and A.I. Lamond. 1993. Assembly of snRNP-containing coiled bodies is regulated in interphase and mitosis - evidence that the coiled body is a kinetic nuclear structure. J. Cell Biol. 120:841-852.
9. Champlin, D.T., and J.T. Lis. 1994. Distribution of B52 within a chromosomal locus depends on the level of transcription. Mol. Biol. Cell. 5:71-79.
10. Champlin, D.T., M. Frasch, H. Saumweber, and J.T. Lis. 1991. Characterization of a Drosophila protein associated with boundaries of transcriptionally active chromatin. Genes & Dev. 5:1611-1621.
11. Dreyfuss, G., M.J. Matunis, S. Pinol-Roma, and C.G. Burd. 1993. hnRNP proteins and the biogenesis of mRNA. Annu. Rev. Biochem. 62:289-321.
12. Edström, J.-E., S. Lindgren, U. Lönn, and L. Rydlander. 1978. Balbiani ring RNA content and half life in nucleus and cytoplasm of Chironomus tentans. Chromosoma (Berl.). 66:33-44.
13. Egyházi, E. 1975. Inhibition of nuclear RNA synthesis at the initiation level, Proc. Natl. Acad. Sci. USA. 72:947-950.
14. Egyházi, E. 1976. Initiation inhibition and reinitiation of the synthesis of heterogeneous nuclear RNA in living cells. Nature (Land.). 262:319-321.
15. Egyházi, E., B. Dancholt, J.-E. Edström, B. Lambert, and U. Ringborg. 1969. Low molecular weight RNA in cell components of Chironomus tentans salivary gland cells. J. Mol. Biol. 44:517-532.
16. Fakan, S. 1994. Perichromatin fibrils are in situ forms of nascent transcripts. Trends Cell Biol. 4:86-90.
17. Fakan, S., G. Leser, and T.E. Martin. 1984. Ultrastructural distribution of nuclear ribonucleoproteins as visualized by immunocytochemistry on thin sections. J. Cell Biol. 98:358-363.
18. Fakan, S., G. Leser, and T.E. Martin. 1986. Immunoelectron microscope visualization of nuclear ribonucleoprotein antigens within spread transcription complexes. J. Cell Biol. 103:1153-1157.
19. Galler, R., L. Rydlander, N. Rieder, H. Kluding, and J.-E. Edström. 1984. Bal biani ring induction in phosphate metabolism. Proc. Natl. Acad. Sci. USA. 81:1448-1452.
20. Ge, H., and J.L. Manley. 1991. A protein factor, ASF, controls cell-specific alternative splicing of SV40 early pre-mRNA in vitro. Cell. 62:25-34.
21. Green, M.R. 1986. Pre-mRNA splicing. Annu. Rev. Genet. 20:671-708.
22. Habets, W.J., M.H. Hoet, B.A.W. De Jong, A. Van der Kemp, and W.J. Van Venrooij. 1989. Mapping of B cell epitopes on small nuclear ribonucleoproteins that react with human autoantibodies as well as with experimentally induced mouse monoclonal antibodies. J. Immunol. 143:2560-2566.
23. Huang, S., and D.L. Spector. 1992. U1 and U2 small nuclear RNAs are present in nuclear speckles. Proc. Natl. Acad. Sci. USA. 89:395-398.
24. Jimenez-Garcia, L.F., and D.L. Spector. 1993. In vivo evidence that transcription and splicing are coordinated by a recruiting mechanism. Cell. 73:47-59.
25. Kiledjian, M., C.G. Burd, M. Görlach, D.S. Portman, and G. Dreyfuss. 1994. In RNA-Protein Interactions, Frontiers in Molecular Biology. K.Nagai, and I.W. Mattaj, editors. IRL Press, Oxford. UK. 128-149.
26. Kiseleva, E., T. Wurtz, N. Visa, and B. Daneholl. 1994. Assembly and disassembly of spliceosomes along a specific pre-messenger RNP fiber. EMBO (Eur. Mol. Biol. Organ.) J. 13:6052-6061.
27. Krainer, A.R., G.C. Conway, and D. Kozak. 1991. The essential pre-mRNA splicing factor SF2 influences 5′ splice site selection by activating proximal sites. Cell. 62:35-42.
28. Lambert, B., and B. Daneholt. 1975. Microanalysis of RNA from defined cellular compartments. Methods Cell Biol. 10:17-47.
29. Lamond, A.I., and M. Carmo-Fonseca. 1993. The coiled body. Trends Cell Biol. 3:198-204.
30. Larsson, S.H., J.-P. Charlieu, K. Miyagawa, D. Engelkamp, M. Rassoulzadegan, A. Ross, F. Cuzin, V. van Heyningen, and N.D. Hastie. 1995. Subnuclear localization of WT1 in splicing or transcription factor domains is regulated by alternative splicing. Cell. 81:391-401.
31. Lendahl, U., and L. Wieslander. 1984. Balbiani ring 6 gene in Chironomus tentans: a diverged member of the Balbiani ring gene family. Cell. 36:1027-1034.
32. Lendahl, U., and L. Wieslander. 1987. Balbiani ring (BR) genes exhibit different patterns of expression during development. Dev. Biol. 121:130-138.
33. Lerner, E.A., M.R. Lerner, C.A. Janeway, and J.A. Steitz. 1981. Monoclonal antibodies to nucleic acid-containing cellular constituents: probes for molecular biology and autoimmune disease. Proc. Natl. Acad. Sci. USA. 78:2737-2741.
34. Mattaj, I.W. 1994. Splicing in space. Nature (Lond.). 372:727-728.
35. Matera, A.G., and D.C. Ward. 1993. Nucleoplasmic organization of small ribonucleoproteins in cultured human cells. J. Cell Biol. 121:715-727.
36. Matunis, E.L., M.J. Matunis, and G. Dreyfuss. 1993. Association of individual hnRNP proteins and snRNPs with nascent transcripts. J. Cell Biol. 121:219-228.
37. Meyer, B., R. Mähr, H.M. Eppenberger, and M. Lezzi. 1983. The activity of Balbiani rings 1 and 2 in salivary glands of Chironomus tentans larvae under different modes of development and after pilocarpine treatment. Dev. Biol. 98:265-277.
38. Nelson, L.G., and B. Daneholt. 1981. Modulation of 75 S RNA synthesis in the Balbiani rings of Chironomus tentans with galactose treatment. Chromosoma (Berl.). 83:265-277.
39. Nevins, J.R. 1983. The pathway of eukaryotic mRNA formation. Annu. Rev. Biochem. 52:441-466.
40. Newman, A.J. 1994. Pre-mRNA splicing. Curr. Opin. Genet. & Develop. 4:298-304.
41. Nyman, U., H. Hallman, G. Hadlaczky, I. Pettersson, G. Sharp, and N.R. Ringertz. 1986. Intranuclear localization of snRNP antigens. J. Cell Biol. 102:137-144.
42. Paulsson, G., U. Lendahl, J. Galli, C. Ericsson, and L. Wieslander. 1990. The Balbiani ring 3 gene in Chironomus tentans has a diverged repetitive structure split by many introns. J. Mol. Biol. 211:331-349.
43. Pettersson, I., M. Hinterberger, T. Mimori, E. Gottliev, and J.A. Steitz. 1984. The structure of mammalian small nuclear ribonucleoproteins. Identification of multiple protein components reactive with anti-(U1) ribonucleoprotein and anti-Sm autoantibodies. J. Biol. Chem. 259:5907-5914.
44. Puvion-Dutilleul, F., J.-P. Bachellerie, N. Visa, and E. Puvion. 1994. Rearrangements of intranuclear structures involved in RNA processing in response to adenovirus infection. J. Cell Sci. 107:1457-1468.
45. Rymond, B.C., and M. Rosbash. 1992. Yeast pre-mRNA splicing. In The Molecular and Cellular Biology of the Yeast Saccharomyces. E.W. Jones, J.R. Pringle, and J.R. Broach, editors. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY. 143-192.
46. Sass, H., and T. Pederson. 1984. Transcription-dependent localization of U1 and U2 small nuclear ribonucleoproteins at major sites of gene activity in polytene chromosomes. J. Mol. Biol. 180:911-926.
47. Sehgal, P.B., E. Derman, G.R. Molloy, I. Tamm, and J.E. Darnell. 1976. 5,6-Dichloro-1-b-D-ribofuranosylbenzimidazole inhibits initiation of nuclear heterogeneous RNA chains in HeLa cells. Science (Wash. DC). 194:431-433.
48. Séraphin, B., and M. Rosbash. 1989. Identification of functional U1 snRNA-pre-mRNA complexes committed to spliceosome assembly and splicing. Cell. 59:349-358.
49. Sharp, P.A. 1994. Split genes and RNA splicing. Cell. 77:805-815.
50. Shatkin, A. 1976. Capping of eukaryotic mRNAs. Cell. 9:645-653.
51. Silver, L.M., C.E.C. Wu, and S.C.R. Elgin. 1978. Immunofluorescent techniques in the analysis of chromosomal proteins. Methods Cell Biol. 18:151-167.
52. Spector, D.L. 1993. Macromolecular domains within the cell nucleus. Annu. Rev. Cell Biol. 9:265-315.
53. Spector, D.L., X.-D. Fu, and T. Maniatis. 1991. Association between distinct pre-mRNA splicing components and the cell nucleus. EMBO (Eur. Mol. Biol. Organ.) J. 10:3467-3481.
54. Spector, D.L., G. Lark, and S. Huang. 1992. Differences in snRNP localization between transformed and nontransformed cells. Mol. Biol. Cell. 3:555-569.
55. Vázquez-Nin, G.H., O.M. Echeverría, S. Fakan, G. Leser, and T.E. Martin. 1990. Immunoelectron microscope localization of snRNPs in the polytene nucleus of salivary glands of Chironomus thummi. Chramosoma (Berl.). 99: 44-51.
56. Vázquez-Nin, G.H., O.M. Echeverría, V. Boutinard Rouelle-Rossier. and S. Fakan. 1993. A new type of ribonucleoprotein constituent of the polytene nucleus of the salivary glands of Chironomus thummi and Ch. tentans. Chromosoma (Berl.). 102:693-699.
57. Visa, N., F. Puvion-Dutilleul, J.-P. Bachellerie, and E. Puvion. 1993. Intranuclear distribution of U1 and U2 snRNAs visualized by high resolution in situ hybridisation: revelation of a novel compartment containing U1 but not U2 snRNA in HeLa cells. Eur. J. Biochem. 60:308-321.
58. Wahle, E. 1995. 3′-end cleavage and polyadenylation of mRNA precursors. Biochim. Biophys. Acta. 1261:183-194.
59. Wallen, P. 1991. Confocal microscopy in chemical neuroanatomy. J. Chem. Neuroanat. 4:387-395.
60. Wieslander, L. 1995. The Balbiani ring multigene family: coding repetitive sequences and evolution of a tissue specific cell function. Prog. Nucleic Acid Res. Mol. Biol. 48:275-313.
61. Wuarin, J., and U. Schibler. 1994. Physical isolation of nascent RNA chains transcribed by RNA polymerase II: evidence for cotranscriptional splicing. Mol. Cell. Biol. 14:7219-7225.
62. Wurtz, T., E. Kiseleva, G. Nacheva, A. Alzhanova-Ericsson, A. Rosén, and B. Daneholt. 1996. Identification of two RNA-binding proteins in Balbiani ring premessenger RNP granules and the presence of these proteins in specific subsets of nuclear ribonucleoprotein particles. Mol. Cell. Biol. 16:1425-1435.
63. Wyss, C. 1982. Chironomus tentans epithelial cell lines sensitive to ecdysteroids. juvenile hormone, insulin and heat shock. Exp. Cell Res. 139:297-307.
64. Xing, Y., C.V. Johnson, P.R. Dobner, and J.B. Lawrence. 1993. Higher level organization of individual gene transcription and RNA splicing: integration of nuclear structure and function. Science (Wash. DC). 259:1326-1330.
65. Zachar, Z., J. Kramer, I.P. Minis, and P.M. Bingham. 1993. Evidence for channeled diffusion of pre-mRNAs during nuclear RNA transport in metazoans. J. Cell Biol. 121:729-742.
66. Zhang, G., K.L. Taneja, R.H. Singer, and M.R.Green. 1994. Localization of pre-mRNA splicing in mammalian nuclei. Nature (Lond.). 372:809-812.