Molecular genetic analysis of the region containing the essential Pseudomonas aeruginosa asd gene encoding aspartate-β-semialdehyde dehydrogenase

Microbiology

Volumn 143, Issue 3, 1997, Pages 899-907

  Hoang, Tung T ^a,b   Williams, Scott ^b   Schweizer, Herbert P ^a,c   Lam, Joseph S ^c  

^a Immunology and Pathology   (United States)

^b UNIVERSITY OF CALGARY   (Canada)

^c UNIVERSITY OF GUELPH   (Canada)

Author keywords

Active site; asd; Chromosomal mapping; Pseudomonas aeruginosa; Selectable marker

Indexed keywords

3 ISOPROPYLMALATE DEHYDROGENASE; ASPARTATE SEMIALDEHYDE DEHYDROGENASE; DIAMINOPIMELIC ACID;

ARTICLE; AUXOTROPHY; BACTERIUM MUTANT; CARBOXY TERMINAL SEQUENCE; CELL WALL; CHROMOSOME; CONSENSUS SEQUENCE; ESCHERICHIA COLI; GENE AMPLIFICATION; GENETIC COMPLEMENTATION; HAEMOPHILUS INFLUENZAE; MOLECULAR CLONING; MOLECULAR GENETICS; NONHUMAN; NUCLEOTIDE SEQUENCE; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; PSEUDOMONAS AERUGINOSA; SEQUENCE HOMOLOGY; VIBRIO;

EID: 0030997256     PISSN: 13500872     EISSN: None     Source Type: Journal    
DOI: 10.1099/00221287-143-3-899     Document Type: Article

References (44)

1. Biellmann, J. P., Eid, P., Hirth, C. & Joernvall, H. (1980). Aspartate-β-semialdehyde dehydrogenase from Escherichia coli. Affinity labeling with the substrate analogue L-2-amino-4-oxo-5-chloropentanoic acid: an example of half-site reactivity. Eur J Biochem 104, 59-64.
2. Bodey, G. P., Bolivar, R., Fainstein, V. & Jadeja, L. (1983). Infections caused by Pseudomonas aeruginosa. Rev Infect Dis 5, 279-313.
3. Cardineau, G. A. & Curtiss, R. (1987). Nucleotide sequence of the asd gene of Streptococcus mutans. J Biol Chem 262, 3344-3353.
4. Chamberlain, J. P. (1979). Fluorographic detection of radioactivity in polyacrylamide gels with the water-soluble fluor, sodium salicylate. Anal Biochem 98, 132-135.
5. R cassettes with the option of Flp-catalyzed excision of the antibiotic-resistance determinant. Gene 158, 9-14.
6. Clepet, C., Borne, F., Krishnapillai, V., Baird, C., Patte, J. C. & Cami, B. (1992). Isolation, organization and expression of the Pseudomonas aeruginosa threonine genes. Mol Microbiol 6, 3109-3119.
7. Cryz, S. J., Jr, Pitt, T. L., Furer, E. & Germaier, R. (1984). Role of lipopolysaccharide in virulence of Pseudomonas aeruginosa. Infect Immun 44, 508-513.
8. Foglino, M., Borne, F., Bally, M., Ball, G. & Patte, J. C. (1995). A direct sulfhydrylation pathway is used for methionine biosynthesis in Pseudomonas aeruginosa. Microbiology 141, 431-439.
9. Govan, J. R. W. (1988). Alginate biosynthesis and other unusual characteristics associated with the pathogenesis of Pseudomonas aeruginosa in cystic fibrosis. In Bacterial Infections of Respiratory and Gastrointestinal Mucosa, pp. 67-96. Edited by W. Donachie, E. Griffiths & J. Stephen. Oxford: IRL Press.
10. Greene, R. C. (1996). Biosynthesis of methionine. In Escherichia coli and Salmonella, pp. 542-560. Edited by F. C. Neidhardt, R. Curtiss, III, J. L. Ingraham and others. Washington, DC: American Society for Microbiology.
11. Haziza, C., Stragier, P. & Patte, J.-C. (1982). Nucleotide sequence of the asd gene of Escherichia coli, absence of a typical attenuation signal. EMBO J 1, 379-384.
12. Hoang, T. T. & Schweizer, H. P. (1997). Leucine biosynthesis in Pseudomonas aeruginosa: identification and characterization of leuB, encoding 3-isopropylmalate dehydrogenase. Mol Gen Genet (in press).
13. Hoiby, N., Pedersen, S. S., Shand, G. H., Doering, G. & Holder, I. A. (1989). Pseudomonas aeruginosa infection. Antibiot Chemother 42, 1-300.
14. Holder, I. A. & Neely, A. N. (1991). The role of proteases in Pseudomonas infections in burns: a current hypothesis. Antibiot Chemother 44, 99-105.
15. Holloway, B. W. & Zhang, C. (1990). Genetic maps. In Locus Maps of Complex Genomes, pp. 2.71-2.78. Edited by S. J. O'Brien. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory.
16. Holloway, B. W., Römling, U. & Tümmler, B. (1994). Genomic mapping of Pseudomonas aeruginosa PAO. Microbiology 140, 2907-2929.
17. Kureishi, A. & Bryan, L. E. (1992). Pre-boiling high GC content, mixed primers with 3′ complementation allows the successful PCR amplification of Pseudomonas aeruginosa DNA. Nucleic Acids Res 20, 1155.
18. Lightfoot, J. & Lam, J. S. (1993). Chromosomal mapping, expression and synthesis of lipopolysaccharide in Pseudomonas aeruginosa: a role for guanosine diphospho (GDP)-D-mannose. Mol Microbiol 8, 771-782.
19. Liss, L. (1987). New M13 host: DH5αF′ competent cells. Focus 9, 13.
20. Makowski, G. S. & Ramsby, M. L. (1993). pH modification to enhance the molecular sieving properties of sodium dodecyl sulfate-10% polyacrylamide gel. Anal Biochem 212, 283-285.
21. Martin, C., Cami, B., Jeenes, D. D., Haas, D. & Patte, J.-C. (1986). Heterologous expression and regulation of the lysA gene of Pseudomonas aeruginosa and Escherichia coli. Mol Gen Genet 203, 430-434.
22. Miller, J. H. (1992). A Short Course in Bacterial Genetics. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory.
23. + expression-cloning vector: stable maintenance and high level expression of cloned genes in a Salmonella vaccine strain. BioTechnology 6, 693-697.
24. Ochsner, U. A., Koch, A. K., Fiechter, A. & Reiser, J. (1994). Isolation and characterization of a regulatory gene affecting rhamnolipid synthesis in Pseudomonas aeruginosa. J Bacteriol 176, 2044-2054.
25. Patte, J.-C. (1996). Biosynthesis of threonine and lysine. In Escherichia coli and Salmonella, pp. 528-541. Edited by F. C. Neidhardt, R. Curtiss, III, J. L. Ingraham and others. Washington, DC: American Society for Microbiology.
26. Rosenberg, M. C. & Court, D. (1979). Regulatory sequences involved in the promotion and termination of RNA transcription. Annu Rev Genet 13, 319-353.
27. Sambrook, J., Fritsch, E. F. & Maniatis, T. (1989). Molecular Cloning: a Laboratory Manual, 2nd edn. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory.
28. Schleifer, K. H. & Kandler, O. (1972). Peptidoglycan types of bacterial cell walls and their taxonomic implications. Bacteriol Rev 36, 407-477.
29. Schweizer, H. P. (1991). The agmR gene, an environmentally responsive gene, complements defective glpR, which encodes the putative activator for glycerol metabolism in Pseudomonas aeruginosa. J Bacteriol 173, 6798-6806.
30. Schweizer, H. P. & Hoang, T. (1995). An improved system for gene replacement and xylE fusion analysis in Pseudomonas aeruginosa. Gene 158, 15-22.
31. Schweizer, H. P. & Po, C. (1994). Cloning and nucleotide sequence of the glpD gene encoding sn-glycerol-3-phosphate dehydrogenase from Pseudomonas aeruginosa. J Bacteriol 176, 2184-2193.
32. Schweizer, H., Sweet, G. & Larson, T. J. (1986). Physical and genetic structure of the glpD-malT interval of the Escherichia coli K-12 chromosome. Mol Gen Genet 202, 488-492.
33. Schweizer, H. P., Klassen, T. R. & Hoang, T. (1996). Improved methods for gene analysis and expression in Pseudomonas. In Molecular Biology of Pseudomonads, pp. 229-237. Edited by T. Nakazawa, K. Furukawa, D. Haas & S. Silver. Washington, DC: American Society for Microbiology.
34. Slauch, J. M., Mahan, M. J. & Mekalanos, J. J. (1994). In vivo expression technology for selection of genes specifically induced in host tissues. Methods Enzymol 235, 481-492.
35. Studier, F. W., Rosenberg, A. H., Dunn, J. J. & Dubendorff, J. W. (1990). Use of T7 RNA polymerase to direct expression of cloned genes. Methods Enzymol 185, 60-89.
36. Tabor, S. (1994). Expression using the T7 RNA polymerase/ promoter system. In Short Protocols in Molecular Biology, pp. 16.1-16.10. Edited by F. M. Ausubel, R. Brent, R. E. Kingston and others. New York: John Wiley.
37. Vasil, M. L. (1986). Pseudomonas aeruginosa: biology, mechanisms of virulence, epidemiology. J Pediatr 108, 800-805.
38. Vasil, M. L., Graham, M. L., Ostroff, R. M., Shortridge, V. D. & Vasil, A. I. (1991). Phospholipase C: molecular biology and contribution to the pathogenesis of Pseudomonas aeruginosa. Antibiot Chemother 44, 34-47.
39. Wang, J., Mushegian, A., Lory, S. & Jin, S. (1996). Large-scale isolation of candidate virulence genes of Pseudomonas aeruginosa by in vivo selection. Proc Natl Acad Sci USA 93, 10434-10439
40. West, S. E. H. & Iglewski, B. H. (1988). Codon usage in Pseudomonas aeruginosa. Nucleic Acids Res 16, 9323.
41. West, S. E. H., Schweizer, H. P., Dall, C., Sample, A. K. & Runyen-Janecky, L. J. (1994). Construction of improved Escherichia-Pseudomonas shuttle vectors derived from pUC18/19 and the sequence of the region required for their replication in Pseudomonas aeruginosa. Gene 128, 81-86.
42. Wieslander, L. (1979). A simple method to recover intact high molecular weight RNA and DNA after electrophoretic separation in low gelling temperature agarose gels. Anal Biochem 98, 305-309.
43. Xiang, C., Wang, H., Shiel, P., Berger, P. & Guerra, D. J. (1994). A modified alkaline lysis miniprep protocol using a single microcentrifuge tube. BioTechniques 17, 30-31.
44. Yanisch-Perron, C., Vieira, J. & Messing, J. (1985). Improved M13 cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors. Gene 33, 103-119.