Characterization of Pseudomonas aeruginosa enoyl-acyl carrier protein reductase (FabI): A target for the antimicrobial triclosan and its role in acylated homoserine lactone synthesis

Journal of Bacteriology

Volumn 181, Issue 17, 1999, Pages 5489-5497

  Hoang, Tung T ^a   Schweizer, Herbert P ^a  

^a Veterinary Dentistry and Oral Surgery   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ACYL CARRIER PROTEIN; ANTIINFECTIVE AGENT; CROTONIC ACID DERIVATIVE; FLAVINE ADENINE NUCLEOTIDE; GLUCOSE DEHYDROGENASE; HOMOSERINE; HYBRID PROTEIN; LACTONE; MUTANT PROTEIN; OXIDOREDUCTASE; POLYHISTIDINE; PYRIDINE NUCLEOTIDE; REDUCED NICOTINAMIDE ADENINE DINUCLEOTIDE; RIBITOL; S ADENOSYLMETHIONINE; TRICLOSAN;

ACYLATION; AMINO ACID SUBSTITUTION; ARTICLE; CHROMOSOME; DRUG TARGETING; ENZYME ACTIVE SITE; ENZYME ACTIVITY; ENZYME ASSAY; ENZYME SUBSTRATE; GENE DISRUPTION; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; PROTEIN PURIFICATION; PSEUDOMONAS AERUGINOSA; SITE DIRECTED MUTAGENESIS; STRUCTURAL GENE;

BACTERIA (MICROORGANISMS); NEGIBACTERIA; PROKARYOTA; PSEUDOMONAS; PSEUDOMONAS AERUGINOSA;

EID: 0032859660     PISSN: 00219193     EISSN: None     Source Type: Journal    
DOI: 10.1128/jb.181.17.5489-5497.1999     Document Type: Article

References (48)

1. Baldock, C., J. B. Rafferty, S. E. Sedelnikova, P. J. Baker, A. R. Stuitje, A. R. Slabas, T. R. Hawkes, and D. W. Rice. 1996. A mechanism of drug action revealed by structural studies of enoyl reductase. Science 274:2107-2110.
2. Banerjee, A., E. Dubnau, A. Quemard, V. Balasubramanian, K. S. Um, T. Wilson, D. Collins, G. de Lisle, and W. R. Jacobs. 1994. inhA, a gene encoding a target for isoniazid and ethionamide in Mycobacterium tuberculosis. Science 263:227-230.
3. Bergler, H., S. Fuchsbichler, G. Hoegenauer, and F. Turnowsky. 1996. The enoyl-[acyl-carrier-protein] reductase (FabI) of Escherichia coli, which catalyzes a key regulatory step in fatty acid biosynthesis, accepts NADH and NADPH as cofactors and is inhibited by palmitoyl-CoA. Eur. J. Biochem. 242:689-694.
4. Bergler, H., G. Hoegenauer, and F. Turnowski. 1992. Sequences of the envM gene and of two mutated alleles in Escherichia coli. J. Gen. Microbiol. 138:2093-2100.
5. Best, E., and V. C. Knauf. 1993. Organization and nucleotide sequence of the genes encoding the biotin carboxyl carrier protein and biotin carboxylase protein of Pseudomonas aeruginosa acetyl coenzyme A carboxylase. J. Bacteriol. 175:6881-6889.
6. Campos-Garcia, J., A. D. Caro, R. Najera, R. M. Miller-Maier, R. A. Al-Tahhan, and G. Soberon-Chavez. 1998. The Pseudomonas aeruginosa rhlG gene encodes an NADPH-dependent β-ketoacyl reductase which is specifically involved in rhamnolipid synthesis. J. Bacteriol. 180:4442-4451.
7. Chen, H., H. Cheng, and M. Bjerkness. 1993. One-step Coomassie Brilliant Blue R-250 staining of proteins in polycarylamide gel. Anal. Biochem. 212: 295-296.
8. Cronan, J. E., and A. L. Klages. 1981. Chemical synthesis of acyl thioesters of acyl carrier protein with native structure. Proc. Natl. Acad. Sci. USA 78:5440-5444.
9. Cronan, J. E., and C. O. Rock. 1996. Biosynthesis of membrane lipids, p. 612-636. In F. C. Neidhardt, R. Curtiss III, J. L. Ingraham, E. C. C. Lin, K. B. Low, B. Magasanik, W. S. Reznikoff, M. Riley, M. Schaechter, and H. E. Umbarger (ed.), Escherichia coli and Salmonella: cellular and molecular biology, 2nd ed. American Society for Microbiology, Washington, D.C.
10. Davies, D. G., M. R. Parsek, J. P. Pearson, B. H. Iglewski, J. W. Costerton, and E. P. Greenberg. 1998. The involvement of cell-to-cell signals in the development of a bacterial biofilm. Science 280:295-298.
11. Dessen, A., A. Quemard, J. S. Blanchard, W. R. Jacobs, and J. C. Sacchettini. 1995. Crystal structure and function of the isoniazid target of Mycobacterium tuberculosis. Science 267:1638-1641.
12. Ellmann, G. L. 1959. Tissue sulfhydryl groups. Anal. Biochem. 82:70-77.
13. Glessner, A., R. S. Smith, B. H. Iglewski, and J. B. Robinson. 1999. Roles of Pseudomonas aeruginosa las and rhl quorum-sensing systems in control of twitching motility. J. Bacteriol. 181:1623-1629.
14. Greenberg, E. P. 1997. Quorum sensing in Gram-negative bacteria. ASM News 63:371-377.
15. Heath, R. J., and C. O. Rock. 1995. Enoyl-acyl carrier protein reductase (fabI) plays a determinant role in completing cycles of fatty acid elongation in Escherichia coli. J. Biol. Chem. 270:26538-26542.
16. Heath, R. J., and C. O. Rock. 1996. Regulation of fatty acid elongation and initiation by acyl-acyl carrier protein in Escherichia coli. J. Biol. Chem. 271:1833-1836.
17. Heath, R. J., and C. O. Rock. 1996. Roles of the FabA and FabZ β-hydroxyacyl-acyl carrier protein dehydratase in Escherichia coli fatty acid biosynthesis. J. Biol. Chem. 271:27795-27801.
18. Heath, R. J., J. R. Rubin, D. R. Holland, E. Zhang, M. E. Snow, and C. O. Rock. 1999. Mechanism of triclosan inhibition of bacterial fatty acid synthesis. J. Biol. Chem. 274:11110-11114.
19. Heath, R. J., Y.-T. Yu, M. A. Shapiro, E. Olson, and C. O. Rock. 1998. Broad spectrum antimicrobial biocides target the FabI component of fatty acid biosynthesis. J. Biol. Chem. 273:30316-30320.
20. Henry, M. F., and J. E. Cronan. 1992. A new mechanism of transcriptional regulation: release of an activator triggered by small molecule binding. Cell 70:671-679.
21. Hoang, T. T., R. R. Karkhoff-Schweizer, A. J. Kutchma, and H. P. Schweizer. 1998. A broad-host-range Flp-FRT recombination system for site-specific excision of chromosomally-located DNA sequences: application for isolation of unmarked Pseudomonas aeruginosa mutants. Gene 212:77-86.
22. Hoang, T. T., and H. P. Schweizer. 1997. Fatty acid biosynthesis in Pseudomonas aeruginosa: cloning and characterization of the fabAB operon encoding β-hydroxyacyl-acyl carrier protein dehydratase (fabA) and β-ketoacyl-acyl carrier protein synthase I (fabB). J. Bacteriol. 179:5326-5332.
23. Kater, M. M., G. M. Konigstein, H. J. J. Nijkamp, and A. R. Stuitje. 1994. The use of a hybrid genetic system to study the functional relationship between prokaryotic and plant multi-enzyme fatty acid synthase complexes. Plant Mol. Biol. 25:771-790.
24. Kutchma, A. J. 1997. Characterization of a gene cluster containing the Pseudomonas aeruginosa fabD, fabG, acpP and fabF genes involved in fatty acid biosynthesis. M.S. thesis. Colorado State University, Fort Collins, Colo.
25. Kutchma, A. J., T. T. Hoang, and H. P. Schweizer. 1999. Characterization of a Pseudomonas aeruginosa fatty acid biosynthetic gene cluster: purification of acyl carrier protein (ACP) and malonyl-coenzyme A:ACP transacylase (FabD). J. Bacteriol. 181:5498-5504.
26. Levy, C. W., A. Roujeinikova, S. Sedelnikova, P. J. Baker, A. R Stuitje, A. R. Slabas, D. W. Rice, and J. B. Rafferty. 1999. Molecular basis of triclosan activity. Nature 398:383-384.
27. Liss, L. 1987. New M13 host: DH5αF' competent cells. Focus 9:13.
28. Magnuson, K., S. Jackowski, C. O. Rock, and J. E. Cronan. 1993. Regulation of fatty acid biosynthesis in Escherichia coli. Microbiol. Rev. 57:522-542.
29. Makowski, G. S., and M. L. Ramsby. 1993. pH modification to enhance the molecular sieving properties of sodium dodecyl sulfate-10% polyacrylamide gel. Anal. Biochem. 212:283-285.
30. McClean, K. H., M. K. Winson, L. Fish, A. Taylor, S. R. Chhabra, M. Camara, M. Daykin, J. H. Lamb, S. Swift, B. W. Bycroft, G. S. A. B. Stewart, and P. Williams. 1998. Quorum sensing and Chromobacterium violaceum: exploitation of violacein production for the detection of N-acylhomoserine lactones. Microbiology 143:3703-3711.
31. McLean, R. J. C., M. Whiteley, D. J. Stickler, and W. C. Fuqua. 1997. Evidence of autoinducer activity in naturally occurring biofilms. FEMS Microbiol. Lett. 154:259-263.
32. McMurry, L. M., P. F. McDermott, and S. B. Levy. 1999. Genetic evidence that InhA of Mycobacterium smegmatis is a target for triclosan. Antimicrob. Agents Chemother. 43:711-713.
33. McMurry, L. M., M. Oethinger, and S. B. Levy. 1998. Triclosan targets lipid synthesis. Nature 394:531-532.
34. Miller, J. H. 1992. A short course in bacterial genetics. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y.
35. Moré, M. I., D. Finger, J. L. Stryker, C. Fuqua, A. Eberhard, and S. C. Winans. 1996. Enzymatic synthesis of a quorum-sensing autoinducer through use of defined substrates. Science 272:1655-1658.
36. Parsek, M. R., A. L. Schaefer, and E. P. Greenberg. 1997. Analysis of random and site-directed mutations in rhlI, a Pseudomonas aeruginosa gene encoding an acylhomoserine lactone synthase. Mol. Microbiol. 26:301-310.
37. Raetz, C. H. R. 1996. Bacterial lipopolysaccharides: a remarkable family of bioactive macroamphiphiles, p. 1035-1063. In F. C. Neidhardt, R. Curtiss III, J. L. Ingraham, E. C. C. Lin, K. B. Low, B. Magasanik, W. S. Reznikoff, M. Riley, M. Schaechter, and H. E. Umbarger (ed.), Escherichia coli and Salmonella: cellular and molecular biology, 2nd ed. American Society for Microbiology, Washington, D.C.
38. Rosenberg, M. C., and D. Court. 1979. Regulatory sequences involved in the promotion and termination of RNA transcription. Annu. Rev. Genet. 13: 319-353.
39. Schaefer, A. L., D. L. Val, B. L. Hanzelka, J. E. Cronan, and E. P. Greenberg. 1996. Generation of cell-to-cell signals in quorum sensing: acyl homoserine lactone synthase activity of a purified Vibrio fischen LuxI protein. Proc. Natl. Acad. Sci. USA 93:9505-9509.
40. Schweizer, H. P. 1991. The agmR gene, an environmentally responsive gene, complements defective glpR, which encodes the putative activator for glycerol metabolism in Pseudomonas aeruginosa. J. Bacteriol. 173:6798-6806.
41. Schweizer, H. P. 1998. Intrinsic resistance to inhibitors of fatty acid biosynthesis in Pseudomonas aeruginosa is due to efflux: application of a novel technique for generation of unmarked chromosomal mutations for the study of efflux systems. Antimicrob. Agents Chemother. 42:394-398.
42. Schweizer, H. P., T. R. Klassen, and T. Hoang. 1996. Improved methods for gene analysis and expression in Pseudomonas, p. 229-237. In T. Nakazawa, K. Furukawa, D. Haas, and S. Silver (ed.), Molecular biology of pseudomonads. American Society for Microbiology, Washington, D.C.
43. Studier, F. W., A. H. Rosenberg, J. J. Dunn, and J. W. Dubendorff. 1990. Use of T7 RNA polymerase to direct expression of cloned genes. Methods Enzymol. 185:60-89.
44. Turnowsky, F., K. Fuchs, C. Jeschek, and G. Hoegenauer. 1989. envM genes of Salmonella typhimurium and Escherichia coli. J. Bacteriol. 171:6555-6565.
45. Val, D. L., and J. E. Cronan. 1998. In vivo evidence that S-adenosylmethionine and fatty acid intermediates are the substrates for the LuxI family of autoinducer synthases. J. Bacteriol. 180:2644-2651.
46. Van Delden, C., and B. H. Iglewski. 1998. Cell-to-cell signaling and Pseudomonas aeruginosa infections. Emerg. Infect. Dis. 4:551-560.
47. Wang, R. F., and S. R. Kushner. 1991. Construction of versatile low-copy-number vectors for cloning, sequencing and gene expression in Escherichia coli. Gene 100:195-199.
48. Weeks, G., and S. J. Wakil. 1968. Studies on the mechanisms of fatty acid synthesis. J. Biol. Chem. 243:1180-1189.