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Volumn 272, Issue 5268, 1996, Pages 1655-1658

Enzymatic synthesis of a quorum-sensing autoinducer through use of defined substrates

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIAL PROTEIN; CARRIER PROTEIN; COENZYME A; HOMOSERINE; LACTONE; PHEROMONE; S ADENOSYLMETHIONINE;

EID: 0029980095     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.272.5268.1655     Document Type: Article
Times cited : (332)

References (35)
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    • 6-Tral using the same native buffer containing 500 mM imidazole. The resulting preparation was judged to be about 90% pure, containing only one detectable contaminant. Dithiothreitol (DTT) and EDTA (1 mM each) were added before freezing at -80°C.
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    • The cell-free supernatant of E. coli BL21/ DE3(pJS101) was assayed for AAI as described in Table 1.
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    • -1. Fractions were assayed for AAI synthesis activity as described in Table 1.
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    • 6-Tral with 500 μM AdoMet and a range of OOACP concentrations from 2.75 nM to 2.75 μM. We assayed AAI as described in Table 1 and analyzed it using double-reciprocal (Lineweaver-Burk) plots.
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    • 2O). The spectrum obtained was similar to that of a CoA control but contained additional resonances characteristic of the acyl chain.
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    • note
    • We thank P. Greenberg and T. Baldwin for helpful discussions and J. Helmann, V. Stewart, and J. Shapleigh for critical reading of this manuscript. We thank J. B. Schineller for providing o-homoserine phosphate. Supported by NIH grant GM42893 and by Dana Summer fellowships to L.D.F.


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