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15844422845
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note
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6-Tral using the same native buffer containing 500 mM imidazole. The resulting preparation was judged to be about 90% pure, containing only one detectable contaminant. Dithiothreitol (DTT) and EDTA (1 mM each) were added before freezing at -80°C.
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20
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15844420320
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note
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The cell-free supernatant of E. coli BL21/ DE3(pJS101) was assayed for AAI as described in Table 1.
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21
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15844370346
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note
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-1. Fractions were assayed for AAI synthesis activity as described in Table 1.
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22
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15844414368
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unpublished data
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M. I. Moré et al., unpublished data.
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Moré, M.I.1
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15844388966
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note
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6-Tral with 500 μM AdoMet and a range of OOACP concentrations from 2.75 nM to 2.75 μM. We assayed AAI as described in Table 1 and analyzed it using double-reciprocal (Lineweaver-Burk) plots.
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31
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15844372810
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note
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2O). The spectrum obtained was similar to that of a CoA control but contained additional resonances characteristic of the acyl chain.
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32
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15844422130
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in preparation
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M. I. Moré et al., in preparation.
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35
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15844423303
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note
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We thank P. Greenberg and T. Baldwin for helpful discussions and J. Helmann, V. Stewart, and J. Shapleigh for critical reading of this manuscript. We thank J. B. Schineller for providing o-homoserine phosphate. Supported by NIH grant GM42893 and by Dana Summer fellowships to L.D.F.
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