Enzymatic synthesis of a quorum-sensing autoinducer through use of defined substrates

Science

Volumn 272, Issue 5268, 1996, Pages 1655-1658

  Moré, Margret I ^a   Finger, L David ^b   Stryker, Joel L ^a   Fuqua, Clay ^c   Eberhard, Anatol ^b   Winans, Stephen C ^a  

^a Department of Population Medicine and Diagnostic Sciences   (United States)

^b ITHACA COLLEGE   (United States)

^c TRINITY UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIAL PROTEIN; CARRIER PROTEIN; COENZYME A; HOMOSERINE; LACTONE; PHEROMONE; S ADENOSYLMETHIONINE;

ARTICLE; CELL DENSITY; CONTROLLED STUDY; GENE EXPRESSION REGULATION; GENE INDUCTION; NONHUMAN; PLASMID; PRIORITY JOURNAL; PROTEIN PURIFICATION; PROTEIN SYNTHESIS; RHIZOBIUM RADIOBACTER;

EID: 0029980095     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.272.5268.1655     Document Type: Article

References (35)

1. R. Wirth, A. Muscholl, G. Wanner, Trends Microbiol. 4, 96 (1996); B. L. Bassler and M. R. Silverman, Two Component Signal Transduction, J. A. Hoch and T. J. Silhavy, Eds. (American Society for Microbiology, Washington, DC, 1995), chap. 28; G. P. C. Salmond, B. W. Bycroft. G. S. A. B. Stewart, P. Williams, Mol. Microbiol. 16, 615 (1995); W. C. Fuqua, S. C. Winans, E. P. Greenberg, J. Bacteriol. 176, 269 (1994).
2. R. Wirth, A. Muscholl, G. Wanner, Trends Microbiol. 4, 96 (1996); B. L. Bassler and M. R. Silverman, Two Component Signal Transduction, J. A. Hoch and T. J. Silhavy, Eds. (American Society for Microbiology, Washington, DC, 1995), chap. 28; G. P. C. Salmond, B. W. Bycroft. G. S. A. B. Stewart, P. Williams, Mol. Microbiol. 16, 615 (1995); W. C. Fuqua, S. C. Winans, E. P. Greenberg, J. Bacteriol. 176, 269 (1994).
3. R. Wirth, A. Muscholl, G. Wanner, Trends Microbiol. 4, 96 (1996); B. L. Bassler and M. R. Silverman, Two Component Signal Transduction, J. A. Hoch and T. J. Silhavy, Eds. (American Society for Microbiology, Washington, DC, 1995), chap. 28; G. P. C. Salmond, B. W. Bycroft. G. S. A. B. Stewart, P. Williams, Mol. Microbiol. 16, 615 (1995); W. C. Fuqua, S. C. Winans, E. P. Greenberg, J. Bacteriol. 176, 269 (1994).
4. R. Wirth, A. Muscholl, G. Wanner, Trends Microbiol. 4, 96 (1996); B. L. Bassler and M. R. Silverman, Two Component Signal Transduction, J. A. Hoch and T. J. Silhavy, Eds. (American Society for Microbiology, Washington, DC, 1995), chap. 28; G. P. C. Salmond, B. W. Bycroft. G. S. A. B. Stewart, P. Williams, Mol. Microbiol. 16, 615 (1995); W. C. Fuqua, S. C. Winans, E. P. Greenberg, J. Bacteriol. 176, 269 (1994).
5. D. M. Sitnikov, J. B. Schineller, T. O. Baldwin, Mol. Microbiol. 17, 801 (1995).
6. A. Eberhard et al., Biochemistry 20, 2444 (1981).
7. Y. Y. Adar and S. Ulitzur, J. Biolumin. Chemilumin. 8, 261 (1993); B. L. Hanzelka and E. P. Greenberg, J. Bacteriol. 177, 815 (1995).
8. Y. Y. Adar and S. Ulitzur, J. Biolumin. Chemilumin. 8, 261 (1993); B. L. Hanzelka and E. P. Greenberg, J. Bacteriol. 177, 815 (1995).
9. H. B. Kaplan and E. P. Greenberg, J. Bacteriol. 163, 1210 (1985).
10. W. C. Fuqua and S. C. Winans, ibid. 176, 2796 (1994).
11. _, Mol. Microbiol., in press; L. Zhang, P. J. Murphy, A. Kerr, M. E. Tate, Nature 362, 446 (1993); K. R. Piper, S. B. Beck von Bodman, S. K. Farrand, ibid., p. 448; I. Hwang et al., Proc. Natl. Acad. Sci. U.S.A. 91, 4639 (1994).
12. _, Mol. Microbiol., in press; L. Zhang, P. J. Murphy, A. Kerr, M. E. Tate, Nature 362, 446 (1993); K. R. Piper, S. B. Beck von Bodman, S. K. Farrand, ibid., p. 448; I. Hwang et al., Proc. Natl. Acad. Sci. U.S.A. 91, 4639 (1994).
13. _, Mol. Microbiol., in press; L. Zhang, P. J. Murphy, A. Kerr, M. E. Tate, Nature 362, 446 (1993); K. R. Piper, S. B. Beck von Bodman, S. K. Farrand, ibid., p. 448; I. Hwang et al., Proc. Natl. Acad. Sci. U.S.A. 91, 4639 (1994).
14. _, Mol. Microbiol., in press; L. Zhang, P. J. Murphy, A. Kerr, M. E. Tate, Nature 362, 446 (1993); K. R. Piper, S. B. Beck von Bodman, S. K. Farrand, ibid., p. 448; I. Hwang et al., Proc. Natl. Acad. Sci. U.S.A. 91, 4639 (1994).
15. J. P. Pearson et al., Proc. Natl. Acad. U.S.A. 91, 197 (1994).
16. A. Eberhard, T. Longin, C. A. Widrig, S. J. Stranick, Arch. Microbiol. 155, 294 (1991).
17. G. W. Huisman and R. Kolter, Science 265, 537 (1994).
18. J. G. Cao and E. A. Meighen, J. Bacteriol. 175, 3856 (1993).
19. 6-Tral using the same native buffer containing 500 mM imidazole. The resulting preparation was judged to be about 90% pure, containing only one detectable contaminant. Dithiothreitol (DTT) and EDTA (1 mM each) were added before freezing at -80°C.
20. The cell-free supernatant of E. coli BL21/ DE3(pJS101) was assayed for AAI as described in Table 1.
21. -1. Fractions were assayed for AAI synthesis activity as described in Table 1.
22. M. I. Moré et al., unpublished data.
23. K. Magnuson, S. Jackowski, C. O. Rock, J. E. Cronan Jr., Microbiol. Rev. 57, 522 (1993).
24. G. D'Agnolo, I. S. Rosenfield, J. Awaya, S. Omura, P. R. Vagelos, Biochim. Biophys. Acta 326, 155 (1973); G. D'Agnolo, I. S. Rosenfield, P. R. Vagelos, J. Biol. Chem. 250, 5283 (1975).
25. G. D'Agnolo, I. S. Rosenfield, J. Awaya, S. Omura, P. R. Vagelos, Biochim. Biophys. Acta 326, 155 (1973); G. D'Agnolo, I. S. Rosenfield, P. R. Vagelos, J. Biol. Chem. 250, 5283 (1975).
26. M. Siggaard-Andersen et al., Proc. Natl. Acad. Sci. U.S.A. 91, 11027 (1994).
27. T. Ouyang and D. R. Walt, J. Og. Chem. 56, 3752 (1991).
28. J. E. Cronan Jr. and A. L. Klages, Proc. Natl. Acad. Sci. U.S.A. 78, 5440 (1981).
29. C. O. Rock and J. E. Cronan Jr., Methods Enzymol. 71, 341 (1981).
30. 6-Tral with 500 μM AdoMet and a range of OOACP concentrations from 2.75 nM to 2.75 μM. We assayed AAI as described in Table 1 and analyzed it using double-reciprocal (Lineweaver-Burk) plots.
31. 2O). The spectrum obtained was similar to that of a CoA control but contained additional resonances characteristic of the acyl chain.
32. M. I. Moré et al., in preparation.
33. J. Tempé, A. Petit, M. Holsters, M. Van Montagu, J. Schell, Proc. Natl. Acad. Sci. U.S.A. 74, 2848 (1977).
34. C. Fuqua and S. C. Winans, J. Bacteriol. 178, 435 (1996).
35. We thank P. Greenberg and T. Baldwin for helpful discussions and J. Helmann, V. Stewart, and J. Shapleigh for critical reading of this manuscript. We thank J. B. Schineller for providing o-homoserine phosphate. Supported by NIH grant GM42893 and by Dana Summer fellowships to L.D.F.