pET-prof, a plasmid for high-level expression of recombinant peptides fused to a birch profilin-derived hexadecapeptide tag: A system for the detection and presentation of recombinant antigens

Gene

Volumn 237, Issue 2, 1999, Pages 333-342

  Pandjaitan, Budhi ^a   Eibensteiner, Petra Bettina ^b   Vrtala, Susanne ^b   Hayek, Brigitte ^b   Grote, Monika ^c   Reichelt, Rudolf ^c   Rumpold, Helmut ^a   Valenta, Rudolf ^b   Spitzauer, Susanne ^a  

^a UNIVERSITY OF VIENNA   (Austria)

^b Institute of General and Experimental Pathology   (Austria)

^c UNIVERSITY OF MÜNSTER   (Germany)

Author keywords

Epitope; Peptide tag; Plasmid; Profilin; Recombinant allergen

Indexed keywords

EPITOPE; IMMUNOGLOBULIN E ANTIBODY; MONOCLONAL ANTIBODY; PEPTIDE; POLLEN ANTIGEN; PROFILIN; PYROXYLIN; RECOMBINANT PROTEIN; RNA POLYMERASE;

AMINO TERMINAL SEQUENCE; ANTIGEN DETECTION; ANTIGEN PRESENTATION; ARTICLE; ENZYME LINKED IMMUNOSORBENT ASSAY; ESCHERICHIA COLI; GENE EXPRESSION SYSTEM; GENE OVEREXPRESSION; HUMAN; IMMUNOBLOTTING; IMMUNOELECTRON MICROSCOPY; NONHUMAN; PLASMID; POLLEN ALLERGY; PRIORITY JOURNAL; PROTEIN IMMOBILIZATION; PROTEIN LOCALIZATION;

ALLERGENS; AMINO ACID SEQUENCE; ANTIBODIES, MONOCLONAL; BASE SEQUENCE; CLONING, MOLECULAR; CONTRACTILE PROTEINS; ENZYME-LINKED IMMUNOSORBENT ASSAY; GENE EXPRESSION; IMMUNOGLOBULIN E; MICROFILAMENT PROTEINS; MICROSCOPY, IMMUNOELECTRON; MOLECULAR SEQUENCE DATA; OLIGOPEPTIDES; PLANT PROTEINS; PLASMIDS; POLLEN; PROFILINS; PROTEIN BINDING; RECOMBINANT FUSION PROTEINS; SOLUBILITY;

EID: 0032834024     PISSN: 03781119     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0378-1119(99)00336-4     Document Type: Article

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