Epitope tag-antibody combination useful for the detection of protein expression in prokaryotic and eukaryotic cells

BioTechniques

Volumn 23, Issue 1, 1997, Pages 96-97

  Rüdiger, Manfred ^a   Jockusch, Brigitte M ^a   Rothkegel, Martin ^a  

^a TECHNICAL UNIVERSITY OF BRAUNSCHWEIG   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

EPITOPE;

AMINO ACID SEQUENCE; ANIMAL CELL; ANTIBODY DETECTION; ANTIBODY SPECIFICITY; ARTICLE; DNA SEQUENCE; EUKARYOTIC CELL; IMMUNOPRECIPITATION; MOLECULAR CLONING; MONITORING; NONHUMAN; PROTEIN EXPRESSION; SEQUENCE HOMOLOGY;

ANIMALIA; EUKARYOTA; PROKARYOTA;

EID: 0342378135     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/97231bm20     Document Type: Article

References (5)

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2. Menkel, A.R., M. Kroemker, P. Bubeck, M. Ronsiek, G. Nikolai and B.M. Jockusch. 1994. Characterization of an F-actin-binding domain in the cytoskeletal protein vinculin. J. Cell Biol. 126:1231-1240.
3. Rothkegel, M., O. Mayboroda, M. Rohde, C. Wucherpfennig, R. Valenta and B.M. Jockusch. 1996. Plant and animal profilins are functionally equivalent and stabilize microfilaments in living animal cells. J. Cell Sci. 109:83-90.
4. Wiedemann, P., K. Giehl, S.C. Almo, A.A. Fedorov, P. Steinberger, M. Rüdiger, M. Ortner, M. Sippl et al. 1996. Molecular and structural analysis of a continous birch profilin epitope defined by a monoclonal antibody. J. Biol. Chem. 271:29915-29921.
5. Zentgraf, H., M. Frey, S. Schwinn, C. Tessmer, B. Willemann, Y. Samstag and I. Velhagen. 1995. Detection of histidine-tagged fusion proteins by using a high-specific mouse monoclonal anti-histidine tag antibody. Nucleic Acids Res. 23:3347-3348.