An artificial enhancer with multiple response elements stimulates prokaryotic transcriptional activation mediated by various regulatory proteins

Molecular and General Genetics

Volumn 262, Issue 2, 1999, Pages 291-301

  Molina Lopez J A ^a   Santero, E ^a  

^a UNIVERSITY OF SEVILLE   (Spain)

Author keywords

(N) RNA polymerase; (N) dependent transcription; Enhancer binding proteins; glnA promoter; Prokaryotic enhancers

Indexed keywords

REGULATOR PROTEIN; RNA POLYMERASE;

ARTICLE; BACTERIAL GENETICS; BINDING SITE; ENHANCER REGION; PRIORITY JOURNAL; PROMOTER REGION; TRANSCRIPTION INITIATION; TRANSCRIPTION REGULATION;

BACTERIAL PROTEINS; BINDING SITES; DNA-BINDING PROTEINS; ENHANCER ELEMENTS (GENETICS); GENETIC ENGINEERING; PII NITROGEN REGULATORY PROTEINS; PLASMIDS; PROKARYOTIC CELLS; PROMOTER REGIONS (GENETICS); RESPONSE ELEMENTS; TRANS-ACTIVATION (GENETICS); TRANS-ACTIVATORS; TRANSCRIPTION FACTORS;

EID: 0032821686     PISSN: 00268925     EISSN: None     Source Type: Journal    
DOI: 10.1007/s004380051086     Document Type: Article

References (54)

1. Abril MA, Ramos JL (1993) Physical organization of the Pseudomonas TOL plasmid upper pathway operon promoter. Sequence and positional requirements for XylR-dependent activation of transcription. Mol Gen Genet 239:281-288
2. Arnott M, Sidoti C, Hill S, Merrick M (1989) Deletion analysis of the nitrogen fixation regulatory gene nifL of Klebsiella pneumoniae. Arch Microbiol 151:180-182
3. Berger DK, Narberhaus F, Kustu S (1994) The catalytic domain of NifA, a bacterial enhancer-binding protein, activates transcription in vitro: activation is inhibited by NifL. Proc Natl Acad Sci USA 91:103-107
4. Brill WJ, Steiner AL, Shah VK (1974) Effect of molybdenum starvation and tungsten on the synthesis of nitrogenase components in Klebsiella pneumoniae. J Bacteriol 118:986-989
5. 54 in exponential silencing of the Pseudomonas putida TOL plasmid Pu promoter. Mol Microbiol 19:7-17
6. Claverie-Martín F, Magasanik B (1992) Positive and negative effects of DNA bending on activation of transcription from a distant site. J Mol Biol 277:996-1008
7. Dworkin J, Jovanovic G, Model P (1997) Role of upstream activation sequences and integration host factor in transcriptional activation by the constitutively active prokaryotic enhancer-binding protein PspF. J Mol Biol 273:377-388
8. Dworkin J, Ninfa AJ, Model P (1998) A protein-induced bend increases the specificity of a prokaryotic enhancer-binding protein. Genes Dev 12:894-900
9. Fernández S, Shingler V, de Lorenzo V (1994) Cross-regulation by XylR and DmpR activators of Pseudomonas putida suggests that transcriptional control of biodegradative operons evolves independently of catabolic genes. J Bacteriol 176:5052-5058
10. Fredrick K, Helmann JD (1997) RNA polymerase sigma factor determines start-site selection but is not required for upstream promoter element activation on heteroduplex (bubble) templates. Proc Natl Acad Sci USA 94:4982-4987
11. Goodman SD, Nash H (1989) Functional replacement of a protein-induced bend in a DNA recombination site. Nature 341:251-254
12. Govantes F, Molina-López JA, Santero E (1996) Mechanism of coordinated synthesis of the antagonistic regulatory proteins NifL and NifA of Klebsiella pneumoniae. J Bacteriol 178:6817-6823
13. Govantes F, Andújar E, Santero E (1998) Mechanism of transla tional coupling in the nifLA operon of Klebsiella pneumoniae. EMBO J 17:2368-2377
14. 54-dependent transcriptional activator, may be negatively controlled by a subdomain in the C-terminal end of its two-component receiver module. Mol Microbiol 13:51-66
15. Holtel A, Marqués S, Möhler I, Jakubzik U, Timmis KN (1994) Carbon source-dependent inhibition of xyl operon during expression of the Pseudomonas putida TOL plasmid. J Bacteriol 176:1773-1776
16. Hoover T, Santero E, Porter S, Kustu S (1990) Integration host factor stimulates interaction of RNA polymerase with NifA, the transcriptional activator for nitrogen fixation operons. Cell 63:11-22
17. 54-dependent transcriptional activator FhlA from Escherichia coli. J Bacteriol 177:2798-2803
18. Joung JK, Koepp DM, Hochschild A (1994) Synergistic activation of transcription by bacteriophage lambda cI protein and E. coli cAMP receptor protein. Science 265:1863-1866
19. Keener J, Wong P, Popham D, Kustu S (1987) A sigma factor and auxiliary proteins required for nitrogen-regulated transcription in enteric bacteria. In: Reznikoff WS (ed) RNA Polymerase and the regulation of transcription. Elsevier Science, Amsterdam, pp 159-175
20. Klose KE, Weiss DS, Kustu S (1993) Glutamate at the site of phosphorylation of nitrogen-regulatory protein NtrC mimics aspartyl-phosphate and activates the protein. J Mol Biol 232:67-78
21. Kustu S, Sei K, Keener J (1986) Nitrogen regulation in enteric bacteria. In: Booth I, Higgins C (eds) Regulation of gene expression. Cambridge University Press, pp 139-154
22. Ledebur H, Nixon T (1992) Tandem DctD-binding sites of the Rhizobium meliloti dctA upstream activating sequence are essential for optimal function despite a 50-to 100-fold difference in affinity for DctD. Mol Microbiol 6:3479-3492
23. 54-dependent transcriptional activator. J Biol Chem 269:20401-20409
24. Macián F, Pérez-Roger I, Armengod ME (1994) An improved vector system for constructing transcriptional lacZ fusions: analysis of the regulation of dnaA, dnaN, recF and gyrF genes of Escherichia coli. Gene 145:17-24
25. MacNeil T, MacNeil D, Tyler B (1982) Fine-structure deletion map and complementation analysis of the glnA-glnL-glnG region in Escherichia coli. J Bacteriol 150:1302-1313
26. Marqués S, Ramos JL, Timmis KN (1993) Analysis of the mRNA structure of the Pseudomonas putida TOL meta fission pathway operon around the transcription initiation point, the xylTE and the xylFJ regions. Biochim Biophys Acta 1216:227-236
27. N) Mol Microbiol 10:903-909
28. Miller JH (1992) A short course in bacterial genetics: a laboratory manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y., pp 72-74
29. 54-dependent nifH promoter of Klebsiella pneumoniae. J Biol Chem 269:25419-25425
30. 54 with the Klebsiella pneumoniae and Rhizobium meliloti nifH promoters. The role of NifA in the formation of an open promoter complex. J Mol Biol 210:65-77
31. 54 bacterial enhancer-binding protein family: mechanism of action and phylogenetic relationship of their functional domains. J Bacteriol 175: 6067-6074
32. Ninfa A, Magasanik B (1986) Covalent modification of the glnG product NRI, by the glnL product, NRII, regulates transcription of the glnALG operon in Escherichia coli. Proc Natl Acad Sci USA 83:5909-5913
33. Ninfa A, Reitzer L, Magasanik B (1987) Initiation of transcription at the bacterial glnAp2 promoter by purified E. coli components is facilitated by enhancers. Cell 50:1039-1046
34. 54-dependent promoter Pu of Pseudomonas putida. Proc Natl Acad Sci USA 92:7277-7281
35. 54-dependent transcriptional activator of Pseudomonas putida. J Mol Biol 258:575-587
36. 54 promoters of the TOL plasmid of Pseudomonas putida. J Mol Biol 258:562-574
37. Pérez-Martín J, de Lorenzo V (1996c) VTR expression cassettes for engineering conditional phenotypes in Pseudomonas: activity of the Pu promoter of the TOL plasmid under limiting concentrations of the XylR activator protein. Gene 172:81-86
38. 54 -dependent promoter Pu of the upper-TOL operon by intrinsically curved sequences. J Biol Chem 269:22657-22662
39. Popham D, Szeto D, Keener J, Kustu S (1989) Function of a bacterial activator protein that binds to transcriptional enhancers. Science 243:629-935
40. Porter S, North A, Wedel A, Kustu S (1993) Oligomerization of NtrC at the glnA enhancer is required for transcriptional activation. Genes Dev 7:2258-2273
41. Prodromou C, Pearl LH (1992) Recursive PCR: a novel technique for total gene synthesis. Protein Eng 5:827-829
42. Reitzer L, Magasanik B (1986) Transcription of glnA in E. coli is stimulated by activator bound to sites far from the promoter. Cell 45:785-792
43. Reitzer L, Moversusas B, Magasanik B (1989) Activation of glnA transcription by nitrogen regulator I (NRI)-phosphate in Escherichia coli: evidence for a long-range physical interaction between NRI-phosphate and RNA polymerase. J Bacteriol 171:5512-5522
44. 54 holoenzyme by scanning force microscopy. J Mol Biol 270:125-138
45. 54-dependent nifH promoter. J Mol Biol 227:602-620
46. Sasse-Dwight S, Gralla J (1988) Probing the Escherichia coli glnALG upstream activation mechanism in vivo. Proc Natl Acad Sci USA 85:8934-8938
47. Scott S, Busby S, Beacham I (1995) Transcriptional co-activation at the ansB promoters: involvement of the activating regions of CRP and FNR when bound in tandem. Mol Microbiol 18:521-531
48. Stalder R, Arber W (1989) Characterization of in vitro constructed IS30-flanked transposons. Gene 76:187-193
49. Su W, Porter S, Kustu S, Echols H (1990) DNA-looping and enhancer activity: association between DNA-bound activator and RNA polymerase at the bacterial glnA promoter. Proc Natl Acad Sci USA 87:5504-5508
50. Sze CC, Moore T, Shingler V (1996) Growth phase-dependent transcription of the sigma(54)-dependent Po promoter controlling the Pseudomonas-derived (methyl)phenol dmp operon of pVI150. J Bacteriol 178:3727-3735
51. Wedel A, Kustu S (1995) The bacterial enhancer-binding protein NTRC is a molecular machine: ATP hydrolysis is coupled to transcriptional activation. Genes Dev 9:2042-2052
52. Wedel A, Weiss D, Popham D, Droge P, Kustu S (1990) A bacterial enhancer functions to tether a transcriptional activator near a promoter. Science 248:486-490
53. Weiss D, Batut L, Klose K, Keener J, Kustu S (1991) The phosphorylated form of the enhancer-binding protein NtrC has an ATPase activity that is essential for activation of transcription. Cell 67:155-167
54. Wyman C, Rombel I, North A, Bustamante C, Kustu S (1997) Unusual oligomerization required for activity of a bacterial enhancer-binding protein. Science 275:1658-1661