VTR expression cassettes for engineering conditional phenotypes in Pseudomonas: Activity of the Pu promoter of the TOL plasmid under limiting concentrations of the XylR activator protein

Gene

Volumn 172, Issue 1, 1996, Pages 81-86

  Pérez Martin, José ^a   De Lorenzo, Victor ^a  

^a CENTRO NACIONAL DE BIOTECNOLOGÍA   (Spain)

Author keywords

Broad host range; Catabolic promoters; Gene dosage; Mini Tn5; Pseudomonas putida

Indexed keywords

REGULATOR PROTEIN; TOLUENE;

ARTICLE; BIODEGRADATION; DNA INTEGRATION; EXPRESSION VECTOR; GENE CASSETTE; GENE INDUCTION; GENETIC ENGINEERING; MOLECULAR CLONING; NONHUMAN; OPEN READING FRAME; PLASMID; PRIORITY JOURNAL; PROMOTER REGION; PROTEIN EXPRESSION; PSEUDOMONAS PUTIDA; RECOMBINANT GENE; RESTRICTION FRAGMENT; TRANSLATION INITIATION;

PSEUDOMONAS; PSEUDOMONAS PUTIDA;

EID: 0040471713     PISSN: 03781119     EISSN: None     Source Type: Journal    
DOI: 10.1016/0378-1119(96)00193-X     Document Type: Article

References (22)

1. Abril, M.A., Michan, C., Timmis, K.N. and Ramos, J.L.: Regulator and enzyme specificities of the TOL plasmid-encoded upper pathway for degradation of aromatic hydrocarbons and expansion of the substrate range of the pathway. J. Bacteriol. 171 (1989) 6782-6790.
2. Alton, N.K. and Vapnek, D.: Nucleotide sequence analysis of the chloramphenicol resistance transposon Tn9. Nature 282 (1979) 864-869.
3. Amann, E., Ochs. B. and Abel, K-J.: Tightly regulated tac promoter vectors useful for the expression of unfused and fused proteins in Escherichia coli. Gene 69 (1988) 301-315.
4. Brosius, J., Dull, T.D., Sleeter, D.D. and Noller, H.F.: Gene organization and primary structure of a ribosomal RNA operator from Escherichia coll. J. Mol. Biol. 148 (1981) 107-127.
5. 54 in exponential silencing of the Pseudomonas putida TOL plasmid Pu promoter. Mol. Microbiol. 19 (1996) 7-17.
6. Cohen, S.N. and Chang, A.C.Y.: Revised interpretation of the origin of the pSC101 plasmid. J. Bacteriol. 132 (1977) 734-737.
7. De Lorenzo, V., Herrero, M., Jakubzik, U. and Timmis, K.N.: Mini-Tn5 transposon derivatives for insertion mutagenesis, promoter probing and chromosomal insertion of cloned DNA in Gram-negative eubacteria. J. Bacteriol. 172 (1990) 6568-6572.
8. 54-dependent Pu promoter of TOL plasmid. EMBO J. 10 (1991) 1159-1167.
9. De Lorenzo, V., Cases, I., Herrero, M. and Timmis, K.N.: Early and late response of TOL promoters to pathway inducers: identification of postexponential promoters in Pseudomonas putida with lacZ-tet bicistronic reporters. J. Bacteriol. 175 (1993) 6902-6907.
10. De Lorenzo, V. and Timmis, K.: Analysis and construction of stable phenotypes in Gram-negative bacteria with Tn5- and Tn10-derived minitransposons. Meth. Enzymol. 235 ( 1994) 386-405.
11. De Lorenzo, V. and Pérez-Martín, J.: Regulatory noise in prokaryotic promoters: how bacteria learn to respond to novel environmental signals. Mol. Microbiol. 19 (1996) 1177-1184.
12. Fernández, S., de Lorenzo, V. and Pérez-Martín, J.: Activation of the transcriptional regulator XylR of Pseudomonas putida by release of repression between functional domains. Mol. Microbiol. 16 (1995) 205-213.
13. Herrero, M., de Lorenzo, V. and Timmis, K.N.: Transposon vectors containing non-antibiotic resistance selection markers for cloning and stable chromosomal insertion of foreign gene in Gram-negative bacteria. J. Bacteriol. 172 (1990) 6557-6567.
14. Hugouvieux, N., Köhler, T., Rekik, M. and Harayama, S.: Growth-phase-dependent expression of the Pseudomonas putida TOL plasmid pWWO catabolic genes. J. Bacteriol. 172 (1990) 6651-6600.
15. Kessler, B., Herrero, M., Timmis, K.M. and de Lorenzo, V.: Genetic evidence that the XylS regulator of the Pseudomonas TOL meta operon controls the Pm promoter through weak DNA-protein interactions. J. Bacteriol. 176 (1994) 3171-3176.
16. Marqués, S. and Ramos, J.L.: Transcriptional control of the Pseudomonas putida TOL plasmid catabolic pathways. Mol. Microbiol. 9 (1993) 923-929.
17. Miller, J.H.: Experiments in Molecular Genetics. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY, 1972.
18. North, A., Klose, K.E., Stedman, K.M. and Kustu, S.: Prokaryotic enhancer-binding proteins reflect eukaryote-like modularity: the puzzle of nitrogen regulatory protein C. J. Bacteriol. 175 (1993) 4267-4273.
19. 54-dependent transcriptional activator of Pseudomonas putida. J. Mol. Biol. 258 (1996) 575-587.
20. Takeshita, S., Sato, M., Toba, M., Masahashi, W. and Hashimoto-Gotoh, T.: High-copy-number and low-copy-number plasmid vectors for lacZ α-complementation and chloramphenicol-or kanamycin-resistance selection. Gene 61 (1987) 63-74.
21. Van der Meer, J.R., de Vos, W.H., Harayama, S. and Zehnder, A.: Molecular mechanisms of genetic adaptation to xenobiotic compounds. Microbiol. Rev. 56 (1992) 677-694.
22. Yanisch-Perron, C., Vieira, J. and Messing, J.: Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors. Gene 33 (1985) 103-109.