Mechanism of translational coupling in the nifLA operon of Klebsiella pneumoniae

EMBO Journal

Volumn 17, Issue 8, 1998, Pages 2368-2377

  Govantes, F ^b   Andujar E ^a   Santero, E ^a  

^a UNIVERSITY OF SEVILLE   (Spain)

^b UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

Downstream box; nif regulation; Reinitiation

Indexed keywords

BACTERIAL RNA;

ARTICLE; CONTROLLED STUDY; ENHANCER REGION; GENE MUTATION; KLEBSIELLA PNEUMONIAE; NONHUMAN; OPERON; PRIORITY JOURNAL; RIBOSOME; RNA SEQUENCE; RNA TRANSLATION; SEQUENCE HOMOLOGY;

BACTERIAL PROTEINS; BASE SEQUENCE; DNA; ISOPROPYL THIOGALACTOSIDE; KLEBSIELLA PNEUMONIAE; MOLECULAR SEQUENCE DATA; MUTAGENESIS, SITE-DIRECTED; NUCLEIC ACID CONFORMATION; OPERON; PROTEIN BIOSYNTHESIS; RIBOSOMES; TRANSCRIPTION FACTORS;

BACTERIA (MICROORGANISMS); KLEBSIELLA PNEUMONIAE; NEGIBACTERIA;

EID: 2642677678     PISSN: 02614189     EISSN: None     Source Type: Journal    
DOI: 10.1093/emboj/17.8.2368     Document Type: Article

References (35)

1. Adhin,M.R. and van Duin,J. (1989) Translational regulation of the lysis gene in RNA bacteriophage fr requires a UUG initiation codon. Mol. Gen. Genet., 218, 137-142.
2. Adhin,M.R. and van Duin,J. (1990) Scanning model for translational reinitiation in eubacteria. J. Mol. Biol., 213, 811-818.
3. Brink,M., Verbeet,M. and de Boer,H. (1993) Formation of the central pseudoknot in 16S rRNA is essential for initiation of translation. EMBO J., 12, 3987-3996.
4. Brink,M., Nels,R., Verbeet,M. and de Boer,H. (1994) Specialized ribosomes allow for the study of mutations in functionally important regions in 16S rRNA. without affecting cell growth. The identification pf functional regions in the central domain of 16S rRNA. J. Mol. Biol., 237, 368-377.
5. Buck,M. (1986) Deletion analysis of the Klebsiella pneumoniae nitrogenase promoter: importance of spacing between conserved sequences around positions -12 and -24 for activation by the nifA and ntrC (glnG) gene products. J. Bacteriol., 166, 545-551.
6. Dallmann,H. and Dunn,S. (1994) Translation through an uncDC mRNA secondary structure governs the level of uncC expression in Escherichia coli. J. Bacteriol., 175, 1242-1250.
7. Draper,D. (1996) Translational initiation. In Neidhart,F.C., Curtis,R.,III, Ingraham,J.L., Lin,E.C.C., Low,K.B., Magasanik,B., Reznikoff,W.S., Riley,M., Schaechter,M. and Humbarger,H.E. (eds), Escherichia coli and Salmonella. Cellular and Molecular Biology. ASM Press, Washington DC. pp. 902-908.
8. Gerstel,B. and McCarthy,J.E.G. (1989) Independent and coupled translation initiation of atp genes in Escherichia coli: experiments using chromosomal and plasmid-bornc lacZ fusions. Mol. Microbiol., 3, 851-859.
9. Govantes,F. and Santero,E. (1996) Transcription termination within the regulatory nifLA operon of Klebsiella pneumoniae. Mol. Gen. Genet., 250, 447-454.
10. Govantes,F., Molina-López,J.A. and Santero,E. (1996) Mechanism of coordinated synthesis of the antagonistic regulatory proteins NifL and NifA of Klebsiella pneumoniae. J. Bacteriol., 178, 6817-6823.
11. Grodzicker,T. and Zipser,D. (1968) A mutation which creates a new site for the re-initiation of polypeptide synthesis in the Z gene of the lac operon of Escherichia coli. J. Mol. Biol., 38, 305-314.
12. Hanahan,D. (1983) Studies on transformation of Escherichia coli with plasmids. J. Mol. Biol., 166, 557-580.
13. Henderson,N., Austin,S. and Dixon,R. (1989) Role of metal ions in negative regulation of nitrogen fixation by the nifL gene product from Klebsiella pneumoniae. Mol. Gen. Genet., 216, 484-491.
14. Hill,S., Kennedy,C. and Kavanagh,E. (1981) Nitrogen fixation gene (nifL) involved in oxygen regulation of nitrogenase synthesis in K. pneumoniae. Nature, 290, 424-426.
15. Hui,A. and de Boer,H.A. (1987) Specialized ribosome system: preferential translation of a single mRNA species by a subpopulation of mutated ribosomes in Escherichia coli. Proc. Natl Acad. Sci. USA, 84, 4762-4766.
16. Hui,A., Jhurani,P. and de Boer,H.A. (1987) Directing ribosomes to a single mRNA species: a method to study ribosomal RNA mutations and their effects on translation of a single messenger in Escherichia coli. Methods Enzymol., 153, 432-452.
17. Ivey-Hoyle,M. and Steege,D.A. (1992) Mutational analysis of an inherently defective translation initiation site. J. Mol. Biol. 224, 1039-1054.
18. Keener,J. and Nomura,M. (1996) Regulation of ribosome synthesis. In Neidhart,F.C., Curtis,R.,III, Ingraham,J.L., Lin,E.C.C., Low,K.B., Magasanik,B., Reznikoff,W.S., Riley,M., Schaechter,M. and Humbarger,H.E. (eds). Escherichia coli and Salmonella. Cellular and Molecular Biology. ASM Press. Washington DC. pp. 1417-1431.
19. Kleiner,D., Paul,W. and Merrick,M. (1988) Construction of multicopy expression vectors for regulated over-production of proteins in Klebsiella pneumoniae and other enteric bacteria. J. Gen. Microbiol., 134, 1779-1784.
20. Kunkel,T.A. (1985) Rapid and efficient site-specific mutagenesis without phenotypic selection. Proc. Natl Acad. Sci. USA, 82, 488-192.
21. Laemmli,U.K. (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, 227, 680-683.
22. Lesage,P., Chiaruttini,C., Graffe,M., Dondon,J., Milet,M. and Springer,M. (1992) Messenger RNA secondary structure and translational coupling in the Escherichia coli operon encoding translational initiation factor IF3 and the ribosomal proteins L35 and L20. J. Mol. Biol., 228, 366-383.
23. MacNeil,T., MacNeil,D., Roberts,G.P., Supiano,M.A. and Brill,W. (1978) Fine-structure mapping and complementation analysis of nif (nitrogen fixation) genes in Klebsiella pneumoniae. J. Bacteriol., 136, 252-266.
24. McCarthy,J.E.G. and Brimacombe,R. (1994) Prokaryotic translation: the interactive pathway leading to initiation. Trends Genet., 10, 402-407.
25. McCarthy,J.E.G. and Gualerzi,C. (1990) Translational control of prokaryotic gene expression. Trends Genet., 6, 78-85.
26. Merrick,M., Hill,S., Hennecke,H., Hanh,M., Dixon,R. and Kennedy,C. (1982) Represser properties of the nifL gene product in Klebsiella pneumoniae. Mol. Gen. Genet., 185, 75-81.
27. Miller,M. (1992) A Short Course in Bacterial Genetics. A Laboratory Manual Cold Spring Harbor Laboratory Press. Cold Spring Harbor, NY, pp. 72-74.
28. Resch,A., Tedin,K., Gründling,A., Mündlein,A. and Bläsi,U. (1996) Downstream box-anti-downstream box interactions are dispensable for translation initiation of leaderless mRNAs. EMBO J., 15, 4740-4748.
29. Rex,G., Surin,B., Besse,G., Schneppe,B. and McCarthy,J.E.G. (1994) The mechanism of translational coupling in Escherichia coli. J. Biol. Chem., 269, 18118-18127.
30. Sarabhai,A. and Brenner,S. (1967) A mutant which reinitiates the polypeptide chain after chain termination. J. Mol. Biol., 27, 145-162.
31. Schmitz,R.A., He,L. and Kustu,S. (1996) Iron is required to relieve inhibitory effects of NifL on transcriptional activation by NifA. J. Bacteriol., 178, 4679-4687.
32. Sprengart,M., Fatscher,H. and Fuchs,E. (1990) The initiation of translation in E. coli: apparent base-pairing between the 16S rRNA and downstream sequence of the mRNA. Nucleic Acids Res., 18, 1719-1723.
33. Sprengart,M.L., Fuchs,E. and Porter,A. (1996) The downstream box: an efficient and independent translation initiation signal in Escherichia coli. EMBO J., 15, 665-674.
34. Tabor,S. (1994) Gene expression using the T7 TNA polymerase/promoter system. In Ausubel,F.M., Brent,R., Kingston,R.E., Moore,D.D., Seidman,J.G., Smith,J.A. and Struhl,K. (eds). Current Protocols in Molecular Biology. John Wiley, New York.
35. Yates,J.L. and Nomura,M. (1981) Feedback regulation of ribosomal protein synthesis in E. coli: localization of the mRNA target sites for represser action of the ribosomal protein L 1. Nature, 294, 190-192.