A PCR based DNA hybridisation capture system for the detection of human cytomegalovirus. A comparative study with other identification methods

Journal of Virological Methods

Volumn 80, Issue 2, 1999, Pages 113-122

  Mansy, Frédéric ^a   Brancart, Françoise ^b   Liesnard, Corinne ^b   Bollen, Alex ^a   Godfroid, Edmond ^a  

^a UNIVERSITÉ LIBRE DE BRUXELLES   (Belgium)

^b ERASME HOSPITAL   (Belgium)

Author keywords

Clinical specimens; CMV; Detection methods; Hybridisation; PCR, Non isotopic labelling

Indexed keywords

ANTIGEN; DNA FRAGMENT; VIRUS DNA;

ANIMAL TISSUE; ARTICLE; BLOOD SAMPLING; BODY FLUID; CEREBROSPINAL FLUID; COLORIMETRY; CYTOMEGALOVIRUS; DNA HYBRIDIZATION; FETUS; HUMAN; LUNG LAVAGE; MAJOR CLINICAL STUDY; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; URINE; VIRUS DETECTION; VIRUS INFECTION; VITREOUS BODY;

ADOLESCENT; ADULT; AGED; AMNIOTIC FLUID; CHILD; CHILD, PRESCHOOL; CYTOMEGALOVIRUS; DNA, VIRAL; ENDOPEPTIDASE K; FEMALE; HUMANS; MALE; MIDDLE AGED; NUCLEIC ACID HYBRIDIZATION; PLACENTA; POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY; VITREOUS BODY;

CUCUMBER MOSAIC VIRUS; HUMAN HERPESVIRUS 5;

EID: 0032809392     PISSN: 01660934     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0166-0934(99)00023-3     Document Type: Article

References (52)

1. Abecassis M.M., Koffron A.J., Kaplan B., Buckingham M., Muldoon J.P., Cribbins A.J., Kaufman D.B., Fryer J.P., Stuart J., Stuart F.P. The role of PCR in the diagnosis and management of CMV in solid organ recipients: what is the predictive value for the development of disease and should PCR be used to guide antiviral therapy? Transplantation. 63(2):1997;275-279.
2. Akhtar N., Ni J., Langston C., Demmler G.J., Towbin J.A. PCR diagnosis of viral pneumonitis from fixed-lung tissue in children. Biochem. Mol. Med. 58(1):1996;66-76.
3. Alford C. Breast milk transmission of cytomegalovirus (CMV) infection. Adv. Exp. Med. Biol. 310:1991;293-299.
4. Bollen A., De Vos M.J., Godfroid E. Automatic synthesis of multifork-like structures for multiple labeling of oligonucleotides: application to diagnosis of Lyme disease. Nucleic Acids. Symp. Ser. 34:1995;235-236.
5. Boppana S.B., Pass R.F., Britt W.J., Stagno S., Alford C.A. Symptomatic congenital cytomegalovirus infection: neonatal morbidity and mortality. Pediatr. Infect. Dis. J. 11(2):1992;93-99.
6. Bruggeman C.A. Cytomegalovirus and latency: an overview. Virchows Arch. B Cell Pathol. Incl. Mol. Pathol. 64(6):1993;325-333.
7. Brumback B.G., Bolejack S.N., Morris M.V., Mohla C., Shutzbank T.E. Comparison of culture and the antigenaemia assay for detection of cytomegalovirus in blood specimens submitted to a reference laboratory. J. Clin. Microbiol. 35(7):1997;1819-1821.
8. Cogswell F.B., Bantar C.E., Hughes T.G., Gu Y., Philipp M.T. Host DNA can interfere with detection of Borrelia burgdorferi in skin biopsy specimens by PCR. J. Clin. Microbiol. 34(4):1996;980-982.
9. De Vos M.J., Van Elsen A., Bollen A. New non nucleosidic phosphoramidites for the solid phase multi-labelling of nucleotides: comb- and multi-fork like structures. Nucleosides Nucleotides. 13:1994;2245-2265.
10. Donner C., Liesnard C., Content J., Busine A., Aderca J., Rodesch F. Prenatal diagnosis of 52 pregnancies at risk for congenital cytomegalovirus infection. Obstet. Gynecol. 82(4 part 1):1993;481-486.
11. Fajac A., Stephan F., Ibrahim A., Gautier E., Bernaudin J.F., Pico J.L. Value of cytomegalovirus detection by PCR in bronchoalveolar lavage routinely performed in asymptomatic bone marrow recipients. Bone Marrow Transplant. 20(7):1997;581-585.
12. Fauville-Dufaux M., Maes N., Severin E., Farin C., Serruys E., Struelens M., Younes N., Vincke J.P., De Vos M.J., Bollen A. et al. Rapid identification of Mycobacterium xenopi from bacterial colonies or 'Bactec' culture by the polymerase chain reaction and a luminescent sandwich hybridization assay. Res. Microbiol. 146(4):1995;349-356.
13. Forbes B.A. Acquisition of cytomegalovirus infection: an update. Clin. Microbiol. Rev. 2(2):1989;204-216.
14. Gerna G., Zipeto D., Parea M., Percivalle E., Zavattoni M., Gaballo A., Milanesi G. Early virus isolation, early structural antigen detection and DNA amplification by the polymerase chain reaction in polymorphonuclear leukocytes from AIDS patients with human cytomegalovirus viraemia. Mol. Cell. Probes. 5(5):1991;365-374.
15. Gerna G., Zipeto D., Parea M., Revello M.G., Silini E., Percivalle E., Zavattoni M., Grossi P., Milanesi G. Monitoring of human cytomegalovirus infections and ganciclovir treatment in heart transplant recipients by determination of viremia, antigenaemia, and DNAemia. J. Infect. Dis. 164(3):1991;488-498.
16. Gerna G., Revello M.G., Percivalle E., Morini F. Comparison of different immunostaining techniques and monoclonal antibodies to the lower matrix phosphoprotein (pp65) for optimal quantitation of human cytomegalovirus antigenaemia. J. Clin. Microbiol. 30(5):1992;1232-1237.
17. Gleaves C.A., Smith T.F., Shuster E.A., Pearson G.R. Rapid detection of cytomegalovirus in MRC-5 cells inoculated with urine specimens by using low-speed centrifugation and monoclonal antibody to an early antigen. J. Clin. Microbiol. 19(6):1984;917-919.
18. Gleaves C.A., Hursh D.A., Meyers J.D. Detection of human cytomegalovirus in clinical specimens by centrifugation culture with a nonhuman cell line. J. Clin. Microbiol. 30(4):1992;1045-1048.
19. Gotti E., Suter F., Baruzzo S., Perani V., Moioli F., Remuzzi G. Early ganciclovir therapy effectively controls viremia and avoids the need for cytomegalovirus (CMV) prophylaxis in renal transplant patients with cytomegalovirus antigenaemia. Clin. Transplant. 10(6 part 1):1996;550-555.
20. Grundy J.E., Ehrnst A., Einsele H., Emery V.C., Hebart H., Prentice H.G., Ljungman P. A three-center European external quality control study of PCR for detection of cytomegalovirus DNA in blood. J. Clin. Microbiol. 34(5):1996;1166-1170.
21. Hansen K.K., Vestbo J., Benfield T., Lundgren J.D., Mathiesen L.R. Rapid detection of cytomegalovirus in bronchoalveolar lavage fluid and serum samples by polymerase chain reaction: correlation of virus isolation and clinical outcome for patients with human immunodeficiency virus infection. Clin. Infect. Dis. 24(5):1997;878-883.
22. Ho M. Epidemiology of cytomegalovirus infections. Rev. Infect. Dis. 12(Suppl. 7):1990;701-710.
23. Imbert-Marcille B.M., Cantarovich D., Ferre-Aubineau V., Richet B., Soulillou J.P., Billaudel S. Usefulness of DNA viral load quantification for cytomegalovirus disease monitoring in renal and pancreas/renal transplant recipients. Transplantation. 63(10):1997;1476-1481.
24. Imbert-Marcille B.M., Robillard N., Poirier A.S., Coste-Burel M., Cantarovich D., Milpied N., Billaudel S. Development of a method for direct quantification of cytomegalovirus antigenaemia by flow cytometry. J. Clin. Microbiol. 35(10):1997;2665-2669.
25. Lage A.P., Fauconnier A., Burette A., Glupczynski Y., Bollen A., Godfroid E. Rapid colourimetric hybridization assay for detecting amplified Helicobacter pylori DNA in gastric biopsy specimens. J. Clin. Microbiol. 34(3):1996;530-533.
26. Landry M.L., Ferguson D. Comparison of quantitative cytomegalovirus antigenaemia assay with culture methods and correlation with clinical disease. J. Clin. Microbiol. 31(11):1993;2851-2856.
27. Leland D.S., Hansing R.L., French M.L. Clinical experience with cytomegalovirus isolation using conventional cell cultures and early antigen detection in centrifugation-enhanced shell vial cultures. J. Clin. Microbiol. 27(6):1989;1159-1162.
28. Lesprit P., Scieux C., Lemann M., Carbonelle E., Modai J., Molina J.M. Use of the cytomegalovirus (CMV) antigenaemia assay for the rapid diagnosis of primary CMV infection in hospitalized adults. Clin. Infect. Dis. 26(3):1998;646-650.
29. Levy R., Najioullah F., Thouvenot D., Bosshard S., Aymard M., Lina B. Evaluation and comparison of PCR and hybridization methods for rapid detection of cytomegalovirus in clinical samples. J. Virol. Methods. 62(2):1996;103-111.
30. Liesnard C., De Wit L., Motte S., Brancart F., Content J. Rapid diagnosis of cytomegalovirus lung infection by DNA amplification in bronchoalveolar lavages. Mol. Cell. Probes. 8(4):1994;273-283.
31. Lipson S.M., Kaplan M.H., Tseng L.F., Mandel F.S. Use of the cytomegalovirus antigenaemia (CMV-Ag) assay for the detection of CMV in the blood of AIDS patients. Can. J. Microbiol. 39(11):1993;1059-1065.
32. Lo C.Y., Ho K.N., Yuen K.Y., Lui S.L., Li F.K., Chan T.M., Lo W.K., Cheng I.K. Diagnosing cytomegalovirus disease in CMV seropositive renal allograft recipients: a comparison between the detection of CMV DNAemia by polymerase chain reaction and antigenaemia by CMV pp65 assay. Clin. Transplant. 11(4):1997;286-293.
33. Manez R., St. George K., Linden P., Martin M., Kusne S., Grossi P., Ho M., Rinaldo C. Diagnosis of cytomegalovirus infections by shell vial assay and conventional cell culture during antiviral prophylaxis. J. Clin. Microbiol. 32(11):1994;2655-2659.
34. Mansy F., Hoyois B., De Vos M.J., Van Elsen A., Bollen A., Godfroid E. Colourimetric solid-phase capture hybridization assay for detection of amplified Borrelia burgdorferi DNA. Biotechniques. 21(1):1996;122-125.
35. Miller M.J., Bovey S., Pado K., Bruckner D.A., Wagar E.A. Application of PCR to multiple specimen types for diagnosis of cytomegalovirus infection: comparison with cell culture and shell vial assay. J. Clin. Microbiol. 32(1):1994;5-10.
36. Murray B.M., Amsterdam D., Gray V., Myers J., Gerbasi J., Venuto R. Monitoring and diagnosis of cytomegalovirus infection in renal transplantation. J. Am. Soc. Nephrol. 8(9):1997;1448-1457.
37. Myers J.B., Amsterdam D. The laboratory diagnosis of cytomegalovirus infections. Immunol. Invest. 26(3):1997;383-394.
38. Nelson C.T., Demmler G.J. Cytomegalovirus infection in the pregnant mother, fetus, and new-born infant. Clin. Perinatol. 24(1):1997;151-160.
39. Patel R., Klein D.W., Espy M.J., Harmsen W.S., Ilstrup D.M., Paya C.V., Smith T.F. Optimization of detection of cytomegalovirus viremia in transplantation recipients by shell vial assay. J. Clin. Microbiol. 33(11):1995;2984-2986.
40. Paya C.V., Wold A.D., Ilstrup D.M., Smith T.F. Evaluation of number of shell vial cell cultures per clinical specimen for rapid diagnosis of cytomegalovirus infection. J. Clin. Microbiol. 26(2):1988;198-200.
41. Perez J.L., Mariscal D., Tubau F., Niubo J., Martin R. Evaluation of the CMV-vue antigenaemia assay for rapid detection of cytomegalovirus in peripheral blood leukocytes. Diagn. Microbiol. Infect. Dis. 19(1):1994;15-18.
42. Podzamczer D., Ferrer E., Garcia A., Ramon J.M., Niubo J., Santin M., Rufi G., Perez J.L., Martin R., Gudiol F. pp65 antigenaemia as a marker of future Cmv disease and mortality in HIV-infected patients. Scand. J. Infect. Dis. 29(3):1997;223-227.
43. Pomeroy C., Englund J.A. Cytomegalovirus: epidemiology and infection control. Am. J. Infect. Control. 15(3):1987;107-119.
44. Raynor B.D. Cytomegalovirus infection in pregnancy. Semin. Perinatol. 17(6):1993;394-402.
45. Reina J., Fernandez-Baca V., Munar M., Blanco I. Isolation of cytomegalovirus from peripheral blood by shell-vial culture. Technical considerations. Enferm. Infect. Microbiol. Clin. 15(7):1997;361-363.
46. Reina Prieto J., Bestard Palmer X., Saurina Gomila J., Gasco Company J., Fernandez-Baca Gutierrez del Alamo V., Munar Roca M. Usefulness of urine and blood cultures for diagnosing cytomegalovirus infection in the kidney transplant recipient. Rev. Clin. Esp. 198(1):1998;3-6.
47. Schafer P., Tenschert W., Gutensohn K., Laufs R. Minimal effect of delayed sample processing on results of quantitative PCR for cytomegalovirus DNA in leukocytes compared to results of an antigenaemia assay. J. Clin. Microbiol. 35(3):1997;741-744.
48. Storch G.A., Ettinger N.A., Ockner D., Wick M.R., Gaudreault-Keener M., Rossiter J., Trulock E.P., Cooper J.D. Quantitative cultures of the cell fraction and supernatant of bronchoalveolar lavage fluid for the diagnosis of cytomegalovirus pneumonitis in lung transplant recipients. J. Infect. Dis. 168(6):1993;1502-1506.
49. Uberti-Foppa C., Lillo F., Terreni M.R., Puglisi A., Guffanti M., Gianotti N., Lazzarin A. Cytomegalovirus pneumonia in AIDS patients: value of cytomegalovirus culture from BAL fluid and correlation with lung disease. Chest. 113(4):1998;919-923.
50. van der Bij W., Schirm J., Torensma R., van Son W.J., Tegzess A.M., The T.H. Comparison between viremia and antigenaemia for detection of cytomegalovirus in blood. J. Clin. Microbiol. 26(12):1988;2531-2535.
51. van der Meer J.T., Drew W.L., Bowden R.A., Galasso G.J., Griffiths P.D., Jabs D.A., Katlama C., Spector S.A., Whitley R.J. Summary of the International Consensus Symposium on Advances in the Diagnosis, Treatment and Prophylaxis and Cytomegalovirus Infection. Antiviral Res. 32(3):1996;119-140.
52. Veal N., Payan C., Fray D., Sarol L., Blanchet O., Kouyoumdjian S., Lunel F. Novel DNA assay for cytomegalovirus detection: comparison with conventional culture and pp65 antigenaemia assay. J. Clin. Microbiol. 34(12):1996;3097-3100.