Value of cytomegalovirus detection by PCR in bronchoalveolar lavage routinely performed in asymptomatic bone marrow recipients

Bone Marrow Transplantation

Volumn 20, Issue 7, 1997, Pages 581-585

  Fajac, A ^a   Stephan F ^a   Ibrahim, A ^b   Gautier, E ^b   Bernaudin, J F ^a   Pico, J L ^b  

^a HÔPITAL TENON   (France)

^b INSTITUT GUSTAVE ROUSSY   (France)

Author keywords

Bone marrow transplantation; Bronchoalveolar lavage; CMV; PCR

Indexed keywords

ANTIVIRUS AGENT;

ADULT; ARTICLE; BLOOD SAMPLING; BONE MARROW TRANSPLANTATION; CLINICAL ARTICLE; CONTROLLED STUDY; CYTOMEGALOVIRUS; CYTOMEGALOVIRUS INFECTION; FEMALE; HUMAN; HUMAN CYTOMEGALOVIRUS; HUMAN TISSUE; INTERMETHOD COMPARISON; LUNG LAVAGE; MALE; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; TREATMENT OUTCOME; VIRUS CULTURE; VIRUS DETECTION;

EID: 1842290930     PISSN: 02683369     EISSN: None     Source Type: Journal    
DOI: 10.1038/sj.bmt.1700941     Document Type: Article

References (19)

1. Krowka MJ, Rosenow EC, Hoagland HC. Pulmonary complications following bone marrow transplantation. Chest 1985; 87: 237-246.
2. Schmidt GM, Horak DA, Niland JC et al. A randomized, controlled trial of prophylactic ganciclovir for cytomegalovirus pulmonary infection in recipients of allogenic bone marrow transplants. New Engl J Med 1991; 324: 1005-1011.
3. Goodrich JM, Mori M, Gleaves CA et al. Early treatment with ganciclovir to prevent cytomegalovirus disease after allogenic bone marrow transplantation. New Engl J Med 1991; 325: 1601-1607.
4. Rabella N, Drew WL. Comparison of conventional and shell vial cultures for detecting cytomegalovirus infection. J Clin Microbiol 1990; 28: 806-807.
5. Sandin RL, Rodriguez ER, Rosenberg G et al. Comparison of sensitivity for human cytomegalovirus of the polymerase chain reaction, traditional tube culture and shell vial assay by sequential dilutions of infected cell lines. J Virol Meth 1991; 32: 181-191.
6. Olive DM, Simsek K, Al-Mufti S. Polymerase chain reaction assay for detection of human cytomegalovirus. J Clin Microbiol 1989; 27: 1238-1242.
7. Jiwa NM, Van Gemert GW, Raap AK et al. Rapid detection of human cytomegalovirus DNA in peripheral blood leukocytes of viremic transplant recipients by the polymerase chain reaction. Transplantation 1989; 48: 72-76.
8. Cassol SA, Poon MC, Pal R et al. Primer-mediated enzymatic amplification of cytomegalovirus (CMV) DNA. Application to the early diagnosis of CMV infection in marrow transplant recipients. J Clin Invest 1989; 83: 1109-1115.
9. Einsele H, Steidle M, Vallbracht A et al. Early occurrence of human cytomegalovirus infection after bone marrow transplantation as demonstrated by the polymerase chain reaction technique. Blood 1991; 77: 1104-1110.
10. Cathomas G, Morris P, Pekle K et al. Rapid diagnosis of cytomegalovirus pneumonia in marrow transplant recipients by bronchoalveolar lavage using the polymerase chain reaction, virus culture, and the direct immunostaining of alveolar cells. Blood 1993; 81: 1909-1914.
11. Hebart H, Muller C, Loffler J et al. Monitoring of CMV infection: a comparison of PCR from whole blood, plasma-PCR, pp65-antigenemia and virus culture in patients after bone marrow transplantation. Bone Marrow Transplant 1996; 17: 861-868.
12. Swenson PD, Kaplan MH. Rapid detection of cytomegalovirus in cell culture by indirect immunoperoxidase staining with monoclonal antibody to an early nuclear antigen. J Clin Microbiol 1985; 21: 669-673.
13. Higuchi R. Simple and rapid preparation of samples for PCR. In: Erlich HA (ed). PCR Technology: Principles and Applications for DNA Amplification. Stockton Press: New York, 1989, pp 31-38.
14. Hennighausen L, Fleckeinstein B. Nuclear factor 1 interacts with five DNA elements in promoter region of the human cytomegalovirus major immediate early gene. EMBO J 1986; 5: 1367-1371.
15. Fajac A, Vidaud M, Lebargy F et al. Evaluation of human cytomegalovirus latency in alveolar macrophages. Am J Respir crit Care Med 1994; 149: 495-499.
16. Drouet E, Boibieux A, Michelson S et al. Polymerase chain reaction detection of cytomegalovirus DNA in peripheral blood leukocytes as a predictor of cytomegalovirus disease in HIV-infected patients. AIDS 1993; 7: 665-668.
17. Meyers JD, Ljungman P, Fisher LD. Cytomegalovirus excretion as a predictor of cytomegalovirus disease after bone marrow transplantation: importance of cytomegalovirus viremia. J Infect Dis 1990; 162: 373-380.
18. Buffone GJ, Forst A, Samo T et al. The diagnosis of CMV pneumonitis in lung and heart/lung transplant patients by PCR compared with traditional laboratory criteria. Transplantation 1993; 56: 342-347.
19. Einsele H, Ehninger G, Hebart H et al. Polymerase chain reaction monitoring reduces the incidence of cytomegalovirus disease and the duration and side effects of antiviral therapy after bone marrow transplantation. Blood 1995; 86: 2815-2820.