Isolation of cytomegalovirus from peripheral blood by shell-vial culture. Technical considerations;Aislamiento de citomegalovirus a partir de sangre periferica mediante cultivo en shell-vial. Consideraciones tecnicas

Enfermedades Infecciosas y Microbiologia Clinica

Volumn 15, Issue 7, 1997, Pages 361-363

  Reina, Jordi ^a   Fernández Baca, Victoria ^a   Munar, María ^a   Blanco, Isabel ^a  

^a HOSPITAL UNIVERSITARIO SON ESPASES   (Spain)

Author keywords

Cytomegalovirus; Immunosuppressed patients; Shell vial culture; Viremia

Indexed keywords

ARTICLE; CYTOMEGALOVIRUS; CYTOMEGALOVIRUS INFECTION; HUMAN; IMMUNE DEFICIENCY; IMMUNOFLUORESCENCE; MAJOR CLINICAL STUDY; VIREMIA; VIRUS CULTURE; VIRUS ISOLATION;

CYTOMEGALOVIRUS; HUMANS; LEUKOCYTE COUNT; NEUTROPHILS; PROSPECTIVE STUDIES; VIROLOGY;

EID: 0030778480     PISSN: 0213005X     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (20)

1. Van den Berg AP, Van der Bij W, Van Son WJ, Anema J, Van der Giessen M, Schrim J et al. Cytomegalovirus antigenemia as useful marker of symptomatic cytomegalovirus infection after renal transplantation. A report of 130 consecutive patients. Transplantation 1989; 48: 991-995.
2. Gallant JE, Moore RD, Richman DD, Keruly J, Chaisson RE. Incidence and natural history of CMV disease in patients with advanced HIV disease treated with zidovudine. J Infect Dis 1992; 166: 1.223-1.227.
3. Crumpacker CS, Ganciclovir. N Engl J Med 1996; 335: 721-729.
4. Jacobson MA, O'Donnell JJ, Mills J. Foscarnet treatment of cytomegalovirus retinitis in patients with the acquired immunodeficiency syndrome. Antimicrob Agents Chemother 1989; 33: 736-741.
5. Rahhal FM, Arévalo F, Chávez de la Paz E, Munguia D, Azen S, Freeman WR. Treatment of cytomegalovirus retinitis with intravitreous cidofovir in patients with AIDS. Ann Intern Med 1996; 125: 98-103.
6. Martin M. Prophylactic cytomegalovirus management strategies. Transplantation Procec 1995; 27: 23-27.
7. Van der Bij W, Torensma R, Van Son WJ, Anema J, Schrim J, Tegzess AM et al. Rapid immunodiagnosis of active cytomegalovirus infection by monoclonal antibody staining of blood leukocytes. J Med Virol 1988; 25: 179-188.
8. Van der Bij W, Schrim J, Torensma R, Van Son WJ, Tegzess AM, The TH. Comparison between viremia and antigenemia for detection of cytomegalovirus in blood. J Clin Microbiol 1988; 26: 2.531-2.535.
9. Landry ML, Ferguson D. Comparison of quantitative cytomegalovirus antigenemia assay with culture methods and correlation with clinical disease. J Clin Microbiol 1993; 31: 2.851-2.856.
10. Lipson SM, Kaplan MH, Tseng LF, Mandel FS. Use of the cytomegalovirus antigenemia (CMV-Ag) assay for the detection of CMV in the blood of AIDS patients. Can J Microbiol 1993; 39: 1.059-1.065.
11. Van der Giessen M, The TH, Van Son WJ. Cytomegalovirus antigenemia assay. J Clin Microbiol 1991; 29: 2.909-2.910.
12. Storch GA, Buller RS, Bailey TC, Ettinger NA, Langlois T, Gaudeault-Keener M et al. Comparison of PCR and pp65 antigenemia assay with quantitative shell vial culture for detection of cytomegalovirus in blood leukocytes from solid-organ transplant recipients. J Clin Microbiol 1994; 32: 997-1.003.
13. Reina J, Blanco I, Munar M. Correlation between human cytomegalovirus quantitative p72 antigenemia and viremia. Clin Diagn Virol 1996; 7: 63-67.
14. Gleaves CA, Smith T, Shuster EA, Pearson GR. Comparison of standard tube and shell vial cell culture techniques for the detection of cytomegalovirus in clinical specimens. J Clin Microbiol 1985; 21: 217-221.
15. Paya CV, Wold AD, Smith TF. Detection of cytomegalovirus infections in specimens other than urine by the shell vial assay and the conventional tube cell cultures. J Clin Microbiol 1987; 25: 755-757.
16. Buller RS, Bailey TC, Ettinger NA, Keener M, Langlois T, Miller JP et al. Use of a modified shell vial technique to quantitate cytomegalovirus viremia in a population of solid-organ transplant recipients. J Clin Microbiol 1992; 30: 2.620-2.624.
17. Gerna G, Revello MG, Percivalle E, Morini F. Comparison of different immunostaining techniques and monoclonal antibodies to the lower matrix phosphoprotein (pp65) for optimal quantitation of human cytomegalovirus antigenemia. J Clun Microbiol 1992; 30: 1.232-1.237.
18. Bennion DW, Carlquist J, Wright LJ. Specimen type as a source of variability in the reproducibility and timing of cytomegalovirus identification by culture. Diagn Microbiol Infect Dis 1995; 21: 203-207.
19. Arens M, Owen J, Hagerty CM, Reed CA, Storch GA. Optimizing recovery of cytomegalovirus in the shell vial culture procedure. Diagn Microbiol Infect Dis 1991; 14: 125-130.
20. Paya CV, Wold AD, Ilstruk DM, Smith T. Evaluation of number of shell vial cell cultures per clinical specimen for rapid diagnosis of cytomegalovirus infection. J Clin Microbiol 1988; 26: 198-200.