Leishmania: Overexpression and comparative structural analysis of the stage-regulated meta 1 gene

Experimental Parasitology

Volumn 92, Issue 3, 1999, Pages 183-191

  Uliana, Silvia R B ^a,b   Goyal, Neena ^a   Freymüller, Edna ^c   Smith, Deborah F ^a  

^a IMPERIAL COLLEGE LONDON   (United Kingdom)

^b UNIVERSITY OF SÃO PAULO   (Brazil)

^c FEDERAL UNIVERSITY OF SÃO PAULO   (Brazil)

Author keywords

Differential gene expression; Leishmania; Mutants; Overexpression; Virulence

Indexed keywords

ANIMAL EXPERIMENT; ANIMAL MODEL; ANIMAL TISSUE; ARTICLE; CONTROLLED STUDY; GENE EXPRESSION; GENE TARGETING; IMMUNOBLOTTING; LEISHMANIA; MOUSE; MUTAGENESIS; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; PROMASTIGOTE; PROTEIN EXPRESSION; STRUCTURE ANALYSIS;

ANIMALIA; LEISHMANIA AMAZONENSIS; LEISHMANIA DONOVANI; LEISHMANIA MAJOR; PROTOZOA;

EID: 0032807567     PISSN: 00144894     EISSN: None     Source Type: Journal    
DOI: 10.1006/expr.1999.4410     Document Type: Article

References (29)

1. Alexander J., Russell D. G. The interaction of Leishmania species with macrophages. Advances in Parasitology. 31:1992;176-254.
2. Ajioka J., Swindle J. The calmodulin-ubiquitin (CUB) genes of Trypanosoma cruzi are essential for parasite viability. Molecular and Biochemical Parasitology. 78:1996;217-225.
3. Bart G., Frame M. J., Carter R., Coombs G. H., Mottram J. D. Cathepsin B-like cysteine proteinase-deficient mutants of Leishmania mexicana. Molecular and Biochemical Parasitology. 88:1997;53-61.
4. Blackwell J. M. Progress in the Leishmania genome project. Transactions of the Royal Society of Tropical Medicine and Hygiene. 91:1997;107-110.
5. Brodin T. N., Heath S., Sacks D. L. Genes selectively expressed in the infectious (metacyclic) stage of Leishmania major promastigotes enconde a potential basic-zipper structural motif. Molecular and Biochemical Parasitology. 52:1992;241-250.
6. Charest H., Matlashewski G. Developmental gene expression in Leishmania donovani: Differential cloning and analysis of an amastigote-stage-specific gene. Molecular and Cellular Biology. 14:1994;2975-2984.
7. Chomczynski P., Sacchi N. Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Analytical Biochemistry. 162:1987;156-159.
8. Coulson R. M. R., Smith D. F. Isolation of genes showing increased or unique expression in the infective promastigotes of Leishmania major. Molecular and Biochemical Parasitology. 40:1990;63-76.
9. Coulson R. M. R., Connor V., Chen J. C., Ajioka J. W. Differential expression of Leishmania major-tubulin genes during the acquisition of promastigote infectivity. Molecular and Biochemical Parasitology. 82:1996;227-236.
10. Cruz A. K., Coburn C., Beverley S. M. Double targeted gene replacement for creating null mutants. Proceedings of the National Academy of Sciences USA. 88:1991;7170-7174.
11. Cruz A. K., Titus R., Beverley S. M. Plasticity in chromosome number and testing of essential genes in Leishmania by targeting. Proceedings of the National Academy of Sciences USA. 90:1993;1599-1603.
12. Dumas C., Ouellette M., Tovar J., Cunningham M. L., Fairlamb A. H., Tamar S., Olivier M., Papadopoulou B. Disruption of the trypanothione reductase gene of Leishmania decreases its ability to survive oxidative stress in macrophages. EMBO Journal. 16:1997;2590-2598.
13. Kapler G. M., Coburn C., Beverley S. M. Stable transfection of the human parasite Leishmania major delineates a 30-kilobase region sufficient for extrachromosomal replication and expression. Molecular and Cellular Biology. 10:1990;1084-1094.
14. Kidane G. Z., Samaras N., Spithill T. W. Cloning of developmentally regulated genes from Leishmania major and expression following heat induction. Journal of Biological Chemistry. 264:1989;4244-4250.
15. McConville M. J., Turco S. J., Ferguson M. A. J., Sacks D. L. Developmental modification of lipophosphoglycan during the differentiation of Leishmania major promastigotes to an infectious stage. EMBO Journal. 11:1992;3593-3600.
16. Mottram J. C., Souza A. E., Hutchison E. J., Carter R., Frame M. J., Coombs G. H. Evidence from disruption of the lmcpb gene array of Leishmania mexicana that cysteine proteinases are virulence factors. Proceedings of the National Academy of Sciences USA. 93:1996;6008-6013.
17. Nourbakhsh F., Uliana S. R. B., Smith D. F. Characterisation and expression of a stage-regulated gene of Leishmania major. Molecular and Biochemical Parasitology. 76:1996;201-213.
18. Papadopoulou B., Roy G., Mourad W., Leblanc E., Oulette M. Changes in folate and pteridine metabolism after disruption of the Leishmania H locus short-chain dehydrogenase gene. Journal of Biological Chemistry. 269:1994;7310-7315.
19. Papadopoulou B., Roy G., Dey S., Rosen B. P., Olivier M., Ouellette M. M. Gene disruption of the P-glycoprotein related gene pgpa of Leishmania tarentolae. Biochemical and Biophysical Research Communications. 224:1996;772-778.
20. Ryan K. A., Dasgupta S., Beverley S. M. Shuttle cosmid vectors for the trypanosomatid parasite Leishmania. Gene. 131:1993;145-150.
21. Sacks D. L., Perkins P. V. Identification of an infective stage of Leishmania promastigotes. Science. 223:1984;1417-1419.
22. Sambrook J., Fritsch E. F., Maniatis T. Molecular Cloning: A Laboratory Manual. 1989;Cold Spring Harbor Laboratory Press, Cold Spring Harbor.
23. Sanger F., Coulson A. R. A rapid method for determining sequences in DNA by primed synthesis with DNA polymerase. Journal of Molecular Biology. 94:1975;441-448.
24. Siman-Tov M. M., Aly R., Shapira M., Jaffe C. L. Cloning from Leishmania major of a developmentally regulated gene, c-lpk2, for the catalytic subunit of the cAMP-dependent protein kinase. Molecular and Biochemical Parasitology. 77:1996;201-215.
25. Stempliuk V. A., Uliana S. R. B., Floter-Winter L. M. Structural and functional comparison of rDNA promoter regions of some Leishmania species. Memórias do Instituto Oswaldo Cruz. 92:1997;176.
26. Uliana S. R. B., Affonso M. H. T., Camargo E. P., Floeter-Winter L. M. Leishmania: Genus identification based on a specific sequence of the 18S ribosomal RNA sequence. Experimental Parasitology. 72:1991;157-163.
27. Uliana S. R. B., Fischer W., Stempliuk V. A., Floeter-Winter L. M. Structural and functional characterisation of the Leishmania amazonensis ribosomal RNA promoter. Molecular and Biochemical Parasitology. 76:1996;245-255.
28. Wiese M. A mitogen-activated protein (MAP) kinase homologue of Leishmania mexicana is essential for parasite survival in the infected host. EMBO Journal. 17:1998;2619-2628.
29. Zhang W., Charest H., Ghedin E., Matlashewski G. Identification and overexpression of the A2 amastigote-specific protein in Leishmania donovani. Molecular and Biochemical Parasitology. 78:1996;79-90.